LOCUS       pDG1i-VSV-blast         8369 bp    DNA     circular     23-JUN-2023
DEFINITION  LMBP 9592.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9592
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-JUN-2023
FEATURES             Location/Qualifiers
     promoter        2238..2303
                     /label=synthetic\EM7\promoter
     CDS             268..927
                     /label=chloramphenicol\resistance
     CDS             1269..1574
                     /label=ccdB
     CDS             2304..2702
                     /label=blasticidin\resistance
     misc_recomb     1615..1739
                     /label=attR2
     misc_recomb     complement(35..159)
                     /label=attR1
     promoter        1851..2133
                     /label=SV40\early\promoter
     rep_origin      2090..2165
                     /label=SV40\ori
     terminator      3094..3168
                     /label=SV40\polyA\early
     promoter        6350..6678
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      3597..4052
                     /label=f1\origin
     CDS             4183..5043
                     /label=ampicillin\resistance
     rep_origin      5214..5805
                     /label=pMB1\ori
     promoter        6078..6161
                     /label=lac\promoter
     promoter        6225..6241
                     /label=T3\promoter
     terminator      complement(3094..3168)
                     /label=SV40\polyA\late
     rep_origin      3304..3379
                     /label=SV40\ori
     promoter        complement(3413..3429)
                     /label=T7\promoter
     misc_feature    6789..6833
                     /label=HIV-1\psi\packaging\signal
     misc_feature    7343..7576
                     /label=HIV-1\Rev\response\element
     terminator      2788..3022
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    2772..2787
                     /label=HIV-1\cPPT
     promoter        8093..8361
                     /label=hCMVTRE\promoter
     misc_feature    1783..1815
                     /label=VSV\epitope
     promoter        167..219
                     /label=lacUV5\promoter
BASE COUNT     2264 a      1888 c      2091 g      2126 t 
ORIGIN
        1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 
       61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 
      121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 
      181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg 
      241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 
      301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 
      361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 
      421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 
      481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 
      541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 
      601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 
      661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 
      721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 
      781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 
      841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 
      901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 
      961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 
     1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg 
     1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 
     1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 
     1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg 
     1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 
     1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 
     1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 
     1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 
     1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 
     1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt 
     1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 
     1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 
     1741 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtacaccga catcgagatg 
     1801 aacagactgg gcaagacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg 
     1861 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc 
     1921 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 
     1981 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 
     2041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 
     2101 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 
     2161 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 
     2221 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 
     2281 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca 
     2341 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca 
     2401 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg 
     2461 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc 
     2521 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt 
     2581 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg 
     2641 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct 
     2701 aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 
     2761 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 
     2821 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 
     2881 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 
     2941 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 
     3001 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 
     3061 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 
     3121 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     3181 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 
     3241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     3301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 
     3361 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga 
     3421 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
     3481 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 
     3541 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 
     3601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     3661 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     3721 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     3781 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     3841 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     3901 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     3961 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     4021 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 
     4081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     4141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 
     4201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
     4261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
     4321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
     4381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
     4441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
     4501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
     4561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
     4621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
     4681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 
     4741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
     4801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
     4861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 
     4921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
     4981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
     5041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 
     5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 
     5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     5341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 
     5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     5821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
     5881 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
     5941 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 
     6001 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 
     6061 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 
     6121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 
     6181 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 
     6241 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt 
     6301 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat 
     6361 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc 
     6421 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc 
     6481 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg 
     6541 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 
     6601 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 
     6661 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga 
     6721 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg 
     6781 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg 
     6841 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag 
     6901 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga 
     6961 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg 
     7021 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt 
     7081 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga 
     7141 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct 
     7201 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag 
     7261 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga 
     7321 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa 
     7381 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta 
     7441 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac 
     7501 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa 
     7561 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg 
     7621 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct 
     7681 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag 
     7741 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa 
     7801 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga 
     7861 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag 
     7921 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg 
     7981 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat 
     8041 tagtgaacgg atctcgacgg tatcgatggt ccgaggttct agacgagttt actccctatc 
     8101 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt 
     8161 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac 
     8221 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag 
     8281 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc 
     8341 gtttagtgaa ccgtcagatc gcaccggtc 
//
