LOCUS pDG1i-HA-blast 8363 bp DNA circular 23-JUN-2023 DEFINITION LMBP 9593. SOURCE ORGANISM COMMENT Accession number: LMBP 9593 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 23-JUN-2023 FEATURES Location/Qualifiers promoter 2232..2297 /label=synthetic\EM7\promoter CDS 268..927 /label=chloramphenicol\resistance CDS 1269..1574 /label=ccdB CDS 2298..2696 /label=blasticidin\resistance misc_recomb 1615..1739 /label=attR2 misc_recomb complement(35..159) /label=attR1 promoter 1845..2127 /label=SV40\early\promoter rep_origin 2084..2159 /label=SV40\ori terminator 3088..3162 /label=SV40\polyA\early promoter 6344..6672 /label=RSV/HIV-1\5'\LTR rep_origin 3591..4046 /label=f1\origin CDS 4177..5037 /label=ampicillin\resistance rep_origin 5208..5799 /label=pMB1\ori promoter 6072..6155 /label=lac\promoter promoter 6219..6235 /label=T3\promoter terminator complement(3088..3162) /label=SV40\polyA\late rep_origin 3298..3373 /label=SV40\ori promoter complement(3407..3423) /label=T7\promoter misc_feature 6783..6827 /label=HIV-1\psi\packaging\signal misc_feature 7337..7570 /label=HIV-1\Rev\response\element terminator 2782..3016 /label=RSV/HIV-1\dU3/3'\LTR misc_feature 2766..2781 /label=HIV-1\cPPT promoter 8087..8355 /label=hCMVTRE\promoter misc_feature 1783..1809 /label=HA\epitope promoter 167..219 /label=lacUV5\promoter BASE COUNT 2257 a 1893 c 2087 g 2126 t ORIGIN 1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg 241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg 1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg 1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt 1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 1741 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtaccccta cgacgtgccc 1801 gactacgcca cgcgtaccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc 1861 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat 1921 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 1981 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 2041 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 2101 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 2161 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 2221 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca 2281 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 2341 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 2401 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 2461 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 2521 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac 2581 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc 2641 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac 2701 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 2761 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg 2821 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 2881 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 2941 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 3001 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 3061 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 3121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 3181 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 3241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 3301 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 3361 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta 3421 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 3481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 3541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg 3601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 3661 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 3721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 3781 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 3841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 3901 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 3961 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 4021 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 4081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 4141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 4201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 4261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 4321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 4381 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 4441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 4501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 4561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 4621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 4681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 4741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 4801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 4861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 4921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 4981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 5041 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 5161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 5341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 5401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 5461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 5521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 5581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 5641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 5701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 5761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 5821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 5881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 5941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 6001 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 6061 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 6121 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 6181 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa 6241 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca 6301 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat 6361 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat 6421 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc 6481 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6541 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6601 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6661 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct 6721 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact 6781 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag 6841 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg 6901 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt 6961 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct 7021 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac 7081 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat 7141 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac 7201 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa 7261 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa 7321 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta 7381 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc 7441 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag 7501 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc 7561 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt 7621 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg 7681 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc 7741 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt 7801 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag 7861 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc 7921 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc 7981 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga 8041 acggatctcg acggtatcga tggtccgagg ttctagacga gtttactccc tatcagtgat 8101 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc 8161 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt 8221 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag 8281 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag 8341 tgaaccgtca gatcgcaccg gtc //