LOCUS       pDG2i-VSV-puro          9855 bp    DNA     circular UNA 23-JUN-2023
DEFINITION  LMBP 9594.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9594
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-JUN-2023
FEATURES             Location/Qualifiers
     CDS             7914..8612
                     /label=rtTA3
     RBS             8678..9250
                     /label=IRES\of\EMCV\polyprotein\gene
     CDS             9253..9855
                     /label=puromycin\resistance
     terminator      397..471
                     /label=SV40\polyA\early
     promoter        3653..3981
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      900..1355
                     /label=f1\origin
     CDS             1486..2346
                     /label=ampicillin\resistance
     rep_origin      2517..3108
                     /label=pMB1\ori
     promoter        3381..3464
                     /label=lac\promoter
     promoter        3528..3544
                     /label=T3\promoter
     terminator      complement(397..471)
                     /label=SV40\polyA\late
     rep_origin      607..682
                     /label=SV40\ori
     promoter        complement(716..732)
                     /label=T7\promoter
     misc_feature    4092..4136
                     /label=HIV-1\psi\packaging\signal
     misc_feature    4646..4879
                     /label=HIV-1\Rev\response\element
     terminator      91..325
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    75..90
                     /label=HIV-1\cPPT
     promoter        5396..5664
                     /label=hCMVTRE\promoter
     CDS             5940..6599
                     /label=chloramphenicol\resistance
     CDS             6941..7246
                     /label=ccdB
     misc_recomb     7287..7411
                     /label=attR2
     misc_recomb     complement(5707..5831)
                     /label=attR1
     promoter        7523..7805
                     /label=SV40\early\promoter
     rep_origin      7762..7837
                     /label=SV40\ori
     misc_feature    7455..7487
                     /label=VSV\epitope
     misc_feature    8622..8655
                     /label=loxP
     promoter        5839..5891
                     /label=lacUV5\promoter
BASE COUNT     2569 a      2383 c      2515 g      2388 t 
ORIGIN
        1 gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat 
       61 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 
      121 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 
      181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 
      241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 
      301 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 
      361 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 
      421 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
      481 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 
      541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
      601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
      661 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag 
      721 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
      781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
      841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 
      901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
      961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     1021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     1081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     1141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     1201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     1261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     1321 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
     1381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     1441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     1501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     1561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     1621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     1681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     1741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     1801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     1861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     1921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     1981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     2041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     2101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     2161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     2221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     2281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     2341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     2401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     2461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     2521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     2581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     2641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     2701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     2761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     2821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     2881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     2941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     3001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     3061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     3121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     3181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     3241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 
     3301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     3361 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
     3421 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
     3481 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 
     3541 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt 
     3601 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg 
     3661 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg 
     3721 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt 
     3781 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct 
     3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc 
     4021 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg 
     4081 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg 
     4141 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt 
     4201 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct 
     4261 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact 
     4321 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac 
     4381 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt 
     4441 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga 
     4501 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 
     4561 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 
     4621 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 
     4681 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 
     4741 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 
     4801 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 
     4861 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 
     4921 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 
     4981 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 
     5041 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 
     5101 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 
     5161 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata 
     5221 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac 
     5281 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc 
     5341 gattagtgaa cggatctcga cggtatcgat ggtccgaggt tctagacgag tttactccct 
     5401 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga 
     5461 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 
     5521 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat 
     5581 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag 
     5641 ctcgtttagt gaaccgtcag atcgcaccgg tcactagtcc agtgtggtgg aattctgcag 
     5701 atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 
     5761 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 
     5821 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt 
     5881 ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa 
     5941 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 
     6001 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 
     6061 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 
     6121 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg 
     6181 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 
     6241 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 
     6301 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 
     6361 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 
     6421 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 
     6481 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg 
     6541 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa 
     6601 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt 
     6661 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat 
     6721 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat 
     6781 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt 
     6841 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa 
     6901 atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac 
     6961 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac 
     7021 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc 
     7081 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga 
     7141 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga 
     7201 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt 
     7261 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt 
     7321 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg 
     7381 tttctcgttc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga 
     7441 gtctagaggg cccgtacacc gacatcgaga tgaacagact gggcaagacg cgtaccggtt 
     7501 agtaatgagt ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 
     7561 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 
     7621 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     7681 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     7741 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     7801 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 
     7861 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg accatgtcta 
     7921 ggctggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg 
     7981 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact 
     8041 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc 
     8101 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg 
     8161 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca 
     8221 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc 
     8281 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg 
     8341 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca 
     8401 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg 
     8461 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt 
     8521 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg 
     8581 atgcccttga cgattttgac cttgacatgc tccccgggta aataacttcg tatagtatac 
     8641 attatacgaa gttatggatc cgcggccgca aattccgccc ctctccctcc ccccccccta 
     8701 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 
     8761 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 
     8821 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 
     8881 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 
     8941 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 
     9001 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 
     9061 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 
     9121 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 
     9181 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 
     9241 cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 
     9301 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 
     9361 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 
     9421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 
     9481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg 
     9541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 
     9601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 
     9661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg 
     9721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 
     9781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 
     9841 aagcccggtg cctga 
//
