LOCUS       pENTR3C-hCARMA2-H171N         5305 bp    DNA     circular SYN  9-JUL-2015
DEFINITION  LMBP 9625.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|739039468|
COMMENT     VNTDBDATE|739039756|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-hCARMA2-H171N|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR3C-hCARMA2-H171N" 0 5305 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "EcoRI" "XhoI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" 
COMMENT         "AciI" "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" 
COMMENT         "AloI" "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" 
COMMENT         "BbeI" "Bbr7I" "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" 
COMMENT         "BciVI" "BclI" "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" 
COMMENT         "BmrI" "BmtI" "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" 
COMMENT         "BsaI" "BsaJI" "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" 
COMMENT         "BsiEI" "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" 
COMMENT         "Bsp1286I" "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" 
COMMENT         "BsrFI" "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" 
COMMENT         "BstF5I" "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" 
COMMENT         "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" 
COMMENT         "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" 
COMMENT         "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XmaI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5305 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 497 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 741 0 3521 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AarI" "cacctgc" 12 16 2 1657 1 2581 1 0 "") 
COMMENT        #3=(CRSite (CStringList) "AatII" "gacgtc" 6 0 3 67 0 801 0 3219 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 491 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 479 0 801 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AceIII" "cagctc" 14 18 18 905 1 1130 1 1166 1 
COMMENT            1187 1 1220 1 1280 1 1391 1 1559 1 1664 1 1685 1 1861 0 1877 0 1889 
COMMENT            1 2573 1 2747 0 2917 1 3359 0 3658 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AciI" "ccgc" 2 4 58 43 0 191 0 200 1 238 1 251 
COMMENT            0 520 0 531 0 583 0 616 0 709 1 852 1 955 1 958 1 1039 0 1149 0 1162 
COMMENT            0 1300 0 1349 1 1387 1 1429 1 1435 1 1602 0 1660 0 1792 1 1801 1 
COMMENT            1897 0 1918 0 1932 0 1963 1 2105 0 2163 1 2176 1 2179 1 2191 0 2265 
COMMENT            1 2312 1 2315 1 2643 1 2662 0 2694 0 2782 0 2818 0 2962 1 2976 0 
COMMENT            3011 1 3151 0 3166 0 3325 1 3460 0 3787 0 4708 0 4717 1 4852 0 4962 
COMMENT            1 5083 1 5102 1 5229 1 5257 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "AclI" "aacgtt" 3 0 1 210 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 7 755 0 800 0 823 1 1106 
COMMENT            0 1211 0 1325 0 4758 1 0 "") 
COMMENT        #10=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 344 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AflIII" "acrygt" 2 0 1 5300 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 1 1276 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 1 2942 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AluI" "agct" 3 0 40 349 0 658 0 874 0 919 0 
COMMENT             970 0 1021 0 1144 0 1180 0 1201 0 1234 0 1294 0 1405 0 1573 0 1678 
COMMENT             0 1699 0 1711 0 1720 0 1851 0 1867 0 1903 0 1914 0 2007 0 2051 0 
COMMENT             2083 0 2110 0 2587 0 2737 0 2812 0 2931 0 3223 0 3307 0 3349 0 3453 
COMMENT             0 3540 0 3642 0 3648 0 4743 0 5000 0 5046 0 5136 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AlwI" "ggatc" 10 11 13 61 0 480 1 493 0 599 0 
COMMENT             2011 1 2191 1 2204 0 3016 1 4044 1 4439 0 4654 1 4656 0 4742 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 16 970 0 1201 0 1255 0 
COMMENT             1375 0 1405 0 1492 0 1639 0 1795 0 1836 0 1954 0 2007 0 2506 0 2812 
COMMENT             0 3234 0 3352 0 4891 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 1 1202 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 357 0 4608 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 2770 0 4986 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "ApoI" "raatty" 2 0 3 497 0 3795 0 3979 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4381 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 4182 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 57 0 350 0 741 0 2032 0 
COMMENT             2302 0 2335 0 2527 0 3521 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 10 1684 0 1761 0 1876 0 
COMMENT             2317 0 2503 0 2608 0 2801 0 2815 0 2884 0 3385 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 485 0 2196 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 7 491 0 960 0 2339 0 2702 
COMMENT             0 2944 0 3332 0 3391 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BanII" "grgcyc" 6 0 11 357 0 1773 0 2041 0 
COMMENT             2085 0 2309 0 2379 0 2728 0 2906 0 3512 0 3837 0 4608 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 3395 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 140 0 748 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 135 0 743 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 1079 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BbvI" "gcagc" 14 18 48 587 1 663 1 807 1 835 
COMMENT             0 915 0 936 1 939 1 957 1 979 0 1098 1 1116 1 1145 1 1176 0 1188 1 
COMMENT             1216 0 1413 1 1432 1 1468 0 1482 1 1539 1 1560 1 1686 1 1707 1 1801 
COMMENT             0 1851 0 1938 0 1994 1 2016 0 2119 0 2130 0 2137 0 2441 1 2574 1 
COMMENT             2627 1 2633 1 2743 0 2746 0 2918 1 3140 1 3346 1 3358 0 3420 0 3629 
COMMENT             1 3657 0 4672 1 4878 1 4881 1 4971 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BccI" "ccatc" 10 11 21 293 1 296 0 565 1 767 
COMMENT             0 905 0 1510 1 1820 0 2317 1 2347 1 2714 0 2797 0 2890 1 2976 0 
COMMENT             3244 1 3272 0 3289 1 3476 0 3643 0 3948 1 4006 0 4447 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BceAI" "acggc" 18 20 11 239 0 555 0 1424 1 
COMMENT             2109 0 2405 1 2461 0 2716 0 3439 0 3457 0 4501 0 4800 1 0 "") 
COMMENT        #41=(CRSite (CStringList) "BcefI" "acggc" 18 19 11 239 0 555 0 1425 1 
COMMENT             2109 0 2406 1 2461 0 2716 0 3439 0 3457 0 4501 0 4801 1 0 "") 
COMMENT        #42=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 2 38 1 1017 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 2 2680 1 5102 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BfaI" "ctag" 2 0 4 46 0 2087 0 3531 0 4807 0 
COMMENT             0 "") 
COMMENT        #46=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 4 1807 0 2237 0 4030 0 
COMMENT             4296 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 710 0 2519 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 3 1100 0 2727 0 3449 0 0 
COMMENT             "") 
COMMENT        #50=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 9 357 0 720 0 2342 0 
COMMENT             2774 0 2949 0 3006 0 3335 0 4608 0 4990 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 3 1890 0 2617 0 3277 0 0 
COMMENT             "") 
COMMENT        #52=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 142 0 1480 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 49 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 1669 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BpmI" "ctggag" 23 21 15 791 0 1043 0 1385 0 
COMMENT             1906 1 2033 0 2264 0 2417 0 2432 0 2483 1 2528 0 2704 1 2890 1 3124 
COMMENT             0 3374 0 3448 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 5 1079 1 2052 1 2201 0 
COMMENT             3026 0 4199 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 6 1730 1 3100 0 3293 1 
COMMENT             4676 0 4917 1 5215 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 1 2913 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 1 3254 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 6 64 0 247 0 798 0 2166 0 
COMMENT             3216 0 3392 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 5 860 0 989 1 1581 1 2022 
COMMENT             1 3372 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 36 504 0 642 0 686 0 807 
COMMENT             0 982 0 1005 0 1281 0 1440 0 1560 0 1764 0 1778 0 1971 0 1991 0 
COMMENT             2032 0 2137 0 2227 0 2301 0 2302 0 2335 0 2409 0 2421 0 2526 0 2562 
COMMENT             0 2635 0 2721 0 2841 0 2940 0 2955 0 2984 0 3005 0 3006 0 3049 0 
COMMENT             3144 0 4053 0 4455 0 5140 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 4 488 0 4317 0 4947 0 
COMMENT             5094 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 3 545 1 1166 1 
COMMENT             3057 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 108 0 276 0 611 0 
COMMENT             1089 1 1424 0 1797 1 2227 1 2288 0 2828 0 3801 1 3885 1 4020 1 4107 
COMMENT             1 4227 1 4538 1 5206 1 0 "") 
COMMENT        #66=(CRSite (CStringList) "BseMII" "ctcag" 16 14 19 134 0 167 1 1070 1 
COMMENT             1114 0 1247 1 1631 0 1871 1 1877 0 2192 1 2385 0 2446 0 2741 0 2777 
COMMENT             0 3017 1 3351 0 3463 0 4213 0 4608 1 5017 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BseRI" "gaggag" 17 15 13 566 0 680 0 683 0 
COMMENT             929 0 1190 0 1304 0 1325 0 2429 0 2987 1 3035 1 3104 0 3356 0 3386 
COMMENT             0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BseYI" "cccagc" 2 6 7 611 0 890 1 1940 0 2888 
COMMENT             0 3156 1 3536 0 4996 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 6 662 1 998 1 1097 1 
COMMENT             2597 1 3220 1 3227 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 2 4182 0 4966 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 7 981 0 1618 0 1746 0 
COMMENT             2085 0 2774 0 2997 0 4990 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 1 595 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 46 234 0 637 0 643 0 
COMMENT             892 0 913 0 931 0 988 0 1006 0 1024 0 1174 0 1306 0 1309 0 1381 0 
COMMENT             1639 0 1662 0 1666 0 1723 0 1764 0 1779 0 1903 0 2071 0 2092 0 2142 
COMMENT             0 2206 0 2308 0 2416 0 2506 0 2563 0 2752 0 2795 0 2871 0 2941 0 
COMMENT             2961 0 2985 0 3115 0 3157 0 3175 0 3246 0 3388 0 4096 0 4428 0 4445 
COMMENT             0 4822 0 5101 0 5267 0 5285 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 14 548 1 857 1 860 0 989 
COMMENT             1 1581 1 1818 1 1866 0 2022 1 2137 0 2158 0 3283 0 3372 1 3665 0 
COMMENT             4198 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 3 1818 1 3283 0 4198 0 0 
COMMENT             "") 
COMMENT        #76=(CRSite (CStringList) "BsmFI" "gggac" 16 20 9 75 0 539 0 1237 1 1622 
COMMENT             0 1936 1 2546 0 2814 0 3131 0 3166 1 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 3 1552 0 4066 1 4143 1 0 
COMMENT             "") 
COMMENT        #78=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 22 357 0 720 0 981 0 
COMMENT             1618 0 1746 0 1773 0 2041 0 2085 0 2309 0 2342 0 2379 0 2728 0 2774 
COMMENT             0 2906 0 2949 0 2997 0 3006 0 3335 0 3512 0 3837 0 4608 0 4990 0 0 
COMMENT             "") 
COMMENT        #79=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 2 1421 0 4606 1 
COMMENT             0 "") 
COMMENT        #80=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 19 133 0 168 1 1071 1 
COMMENT             1113 0 1248 1 1630 0 1872 1 1876 0 2193 1 2384 0 2445 0 2740 0 2776 
COMMENT             0 3018 1 3350 0 3462 0 4212 0 4609 1 5018 1 0 "") 
COMMENT        #81=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BspHI" "tcatga" 2 0 1 3706 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "BspMI" "acctgc" 11 15 3 639 0 1657 1 2581 1 0 
COMMENT             "") 
COMMENT        #84=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 4 200 1 1435 1 1920 0 
COMMENT             2163 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 6 422 0 1129 0 2237 1 
COMMENT             2596 1 3572 1 3680 1 0 "") 
COMMENT        #86=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 1 4136 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 3 441 0 1956 0 3547 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "BsrI" "actgg" 7 5 10 137 0 487 0 549 0 1475 0 
COMMENT             2129 1 2878 1 3973 0 4773 0 4885 1 4898 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BssSI" "cacgag" 2 6 2 2999 1 5127 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 1 1201 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 4 811 0 2209 0 2296 0 
COMMENT             3160 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 18 288 1 304 0 866 1 1291 
COMMENT             0 1416 1 1809 0 2239 0 2286 1 2544 1 2706 1 2748 1 2901 0 3123 1 
COMMENT             3255 0 3270 1 3635 1 3813 0 4439 1 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstKTI" "gatc" 4 0 14 56 0 488 0 594 0 2019 0 
COMMENT             2199 0 2253 0 3024 0 4052 0 4182 0 4434 0 4643 0 4651 0 4662 0 4737 
COMMENT             0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 50 97 0 265 0 638 0 770 0 
COMMENT             809 0 887 0 926 0 983 0 989 0 1274 0 1346 0 1376 0 1562 0 1592 0 
COMMENT             1640 0 1688 0 1759 0 1765 0 1993 0 2012 0 2072 0 2139 0 2207 0 2228 
COMMENT             0 2396 0 2411 0 2501 0 2507 0 2558 0 2636 0 2722 0 2753 0 2942 0 
COMMENT             2957 0 3007 0 3068 0 3086 0 3103 0 3128 0 3212 0 3353 0 3383 0 3413 
COMMENT             0 3437 0 3464 0 4072 0 4429 0 5141 0 5154 0 5275 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "BstUI" "cgcg" 3 0 11 1387 0 1604 0 1899 0 
COMMENT             1934 0 2664 0 2696 0 3789 0 3839 0 4184 0 4676 0 5257 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2896 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 8 53 0 485 0 2016 0 2196 
COMMENT             0 3021 0 4431 0 4648 0 4659 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 1 1256 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 5 504 0 1440 0 1778 0 
COMMENT              2421 0 2562 0 0 "") 
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COMMENT              3522 0 3781 0 4147 0 4550 0 5202 0 0 "") 
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COMMENT              2965 0 3153 0 3789 0 5105 0 5260 0 0 "") 
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COMMENT              308 0 (CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1 
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COMMENT        #279=(CFSignal (CObList) "hCARMA2" 4 0 0 505 3519 0 (CStringList) 
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COMMENT                              "BplI: 1 site") 38 1 
COMMENT                             "NNNNNNNNNNNNNNGAGNNNNNCTCNNNNNNNNNNNNNN" 
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COMMENT                              "BstXI: 1 site") 8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                       #437=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276424 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstYI: 8 sites") 1 5 "RGATCY" 
COMMENT                             "YCTAGR") 
COMMENT                       #438=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276456 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstZ17I: 0 sites") 3 3 "GTATAC" 
COMMENT                             "CATATG") 
COMMENT                       #439=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276624 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #440=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #101# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #441=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #101# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1256 " 1))))) 
COMMENT                              "Bsu36I: 1 site") 2 5 "CCTNAGG" "GGANTCC") 
COMMENT                       #442=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276656 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtgI: 5 sites") 1 5 "CCRYGG" 
COMMENT                             "GGYRCC") 
COMMENT                       #443=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276688 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BthCI: 66 sites") 4 1 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #444=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276720 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtsI: 3 sites") 8 6 "GCAGTGNNN" 
COMMENT                             "CGTCACNNN") 
COMMENT                       #445=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276752 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Cac8I: 41 sites") 3 3 "GCNNGC" 
COMMENT                             "CGNNCG") 
COMMENT                       #446=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276784 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CdiI: 10 sites") 4 4 "CATCG" 
COMMENT                             "GTAGC") 
COMMENT                       #447=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276816 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ChaI: 14 sites") 5 1 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #448=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276848 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjeI: 9 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNCANNNNNNNNNNNNNNNN") 
COMMENT                       #449=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276880 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjePI: 11 sites") 26 20 
COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNNAGNNNNNNNNNNNNNNN") 
COMMENT                       #450=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34276912 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                             "TAGCTA") 
COMMENT                       #451=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277080 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Csp6I: 9 sites") 1 3 "GTAC" 
COMMENT                             "CATG") 
COMMENT                       #452=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277112 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviAII: 24 sites") 1 3 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #453=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277144 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviJI: 152 sites") 2 2 "RGCY" 
COMMENT                             "YCGR") 
COMMENT                       #454=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277176 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DdeI: 22 sites") 1 4 "CTNAG" 
COMMENT                             "GANTC") 
COMMENT                       #455=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277208 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DpnI: 14 sites") 2 2 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #456=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277240 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #457=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #116# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #458=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #116# 1) 1 2 2 0 
COMMENT                                              191) " N1: 462 " 1))))) 
COMMENT                              "DraI: 1 site") 3 3 "TTTAAA" "AAATTT") 
COMMENT                       #459=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277272 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #460=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #117# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #461=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #117# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2665 " 1))))) 
COMMENT                              "DraIII: 1 site") 6 3 "CACNNNGTG" "GTGNNNCAC") 
COMMENT                       #462=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277304 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #463=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #118# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #464=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #118# 1) 1 2 2 0 
COMMENT                                              191) " N1: 5198 " 1))))) 
COMMENT                              "DrdI: 1 site") 7 5 "GACNNNNNNGTC" "CTGNNNNNNCAG") 
COMMENT                       #465=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277336 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EaeI: 4 sites") 1 5 "YGGCCR" 
COMMENT                             "RCCGGY") 
COMMENT                       #466=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277368 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EagI: 0 sites") 1 5 "CGGCCG" 
COMMENT                             "GCCGGC") 
COMMENT                       #467=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277536 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EarI: 4 sites") 7 10 
COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
COMMENT                       #468=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277568 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EciI: 7 sites") 17 15 
COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
COMMENT                       #469=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277600 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Eco57MI: 22 sites") 22 20 
COMMENT                             "CTGRAGNNNNNNNNNNNNNNNNN" "GACYTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #470=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277632 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EcoHI: 23 sites") 1 6 "NCCSGGN" 
COMMENT                             "NGGSCCN") 
COMMENT                       #471=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277664 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #472=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #125# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #473=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #125# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2083 " 1))))) 
COMMENT                              "EcoICRI: 1 site") 3 3 "GAGCTC" "CTCGAG") 
COMMENT                       #474=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277696 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EcoNI: 10 sites") 5 6 
COMMENT                             "CCTNNNNNAGG" "GGANNNNNTCC") 
COMMENT                       #475=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277728 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EcoO109I: 6 sites") 2 5 "RGGNCCY" 
COMMENT                             "YCCNGGR") 
COMMENT                       #476=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 24862496 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #477=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #478=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                             " N1: 497 " 1))))) "EcoRI: 1 site") 
COMMENT                             1 5 "GAATTC" "CTTAAG") 
COMMENT                       #479=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277768 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #480=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #128# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #481=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #128# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3528 " 1))))) 
COMMENT                              "EcoRV: 1 site") 3 3 "GATATC" "CTATAG") 
COMMENT                       #482=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277808 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "EsaBC3I: 9 sites") 2 2 "TCGA" 
COMMENT                             "AGCT") 
COMMENT                       #483=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34277840 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FalI: 2 sites") 38 1 
COMMENT                             "NNNNNNNNNNNNNNAAGNNNNNCTTNNNNNNNNNNNNNN" 
COMMENT                             "NNNNNNNNNNNNNNTTCNNNNNGAANNNNNNNNNNNNNN") 
COMMENT                       #484=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278008 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FatI: 24 sites") 1 5 "NCATGN" 
COMMENT                             "NGTACN") 
COMMENT                       #485=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278040 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FauI: 13 sites") 9 11 
COMMENT                             "CCCGCNNNNNNN" "GGGCGNNNNNNN") 
COMMENT                       #486=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278072 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FmuI: 31 sites") 4 1 "GGNCC" 
COMMENT                             "CCNGG") 
COMMENT                       #487=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278104 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Fnu4HI: 66 sites") 2 3 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #488=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278136 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FokI: 18 sites") 14 18 
COMMENT                             "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
COMMENT                       #489=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278168 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FseI: 0 sites") 6 2 "GGCCGGCC" 
COMMENT                             "CCGGCCGG") 
COMMENT                       #490=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278200 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FspAI: 0 sites") 4 4 "RTGCGCAY" 
COMMENT                             "YACGCGTR") 
COMMENT                       #491=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #492=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #138# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #493=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #138# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1703 " 1))))) 
COMMENT                              "FspI: 1 site") 3 3 "TGCGCA" "ACGCGT") 
COMMENT                       #494=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278264 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "GdiII: 3 sites") 1 5 "CGGCCR" 
COMMENT                             "GCCGGY") 
COMMENT                       #495=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278296 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HaeI: 15 sites") 3 3 "WGGCCW" 
COMMENT                             "WCCGGW") 
COMMENT                       #496=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278464 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HaeII: 3 sites") 5 1 "RGCGCY" 
COMMENT                             "YCGCGR") 
COMMENT                       #497=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278496 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HaeIII: 42 sites") 2 2 "GGCC" 
COMMENT                             "CCGG") 
COMMENT                       #498=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HaeIV: 2 sites") 40 1 
COMMENT                             "NNNNNNNNNNNNNNNGAYNNNNNRTCNNNNNNNNNNNNNNN" 
COMMENT                             "NNNNNNNNNNNNNNNCTRNNNNNYAGNNNNNNNNNNNNNNN") 
COMMENT                       #499=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278560 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HgaI: 5 sites") 10 15 
COMMENT                             "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN") 
COMMENT                       #500=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278592 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HhaI: 22 sites") 3 1 "GCGC" 
COMMENT                             "CGCG") 
COMMENT                       #501=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278624 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Hin4I: 6 sites") 24 19 
COMMENT                             "GAYNNNNNVTCNNNNNNNNNNNNNN" 
COMMENT                             "CTRNNNNNBAGNNNNNNNNNNNNNN") 
COMMENT                       #502=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278656 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HincII: 5 sites") 3 3 "GTYRAC" 
COMMENT                             "CARYTG") 
COMMENT                       #503=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278688 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                             "TTCGAA") 
COMMENT                       #504=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278720 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HinfI: 16 sites") 1 4 "GANTC" 
COMMENT                             "CTNAG") 
COMMENT                       #505=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278752 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HinP1I: 22 sites") 1 3 "GCGC" 
COMMENT                             "CGCG") 
COMMENT                       #506=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278920 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                             "CAATTG") 
COMMENT                       #507=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278952 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpaII: 26 sites") 1 3 "CCGG" 
COMMENT                             "GGCC") 
COMMENT                       #508=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34278984 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HphI: 16 sites") 13 12 
COMMENT                             "GGTGANNNNNNNNN" "CCACTNNNNNNNNN") 
COMMENT                       #509=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279016 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Hpy188I: 17 sites") 3 2 "TCNGA" 
COMMENT                             "AGNCT") 
COMMENT                       #510=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279048 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Hpy188III: 23 sites") 2 4 
COMMENT                             "TCNNGA" "AGNNCT") 
COMMENT                       #511=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279088 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Hpy8I: 14 sites") 3 3 "GTNNAC" 
COMMENT                             "CANNTG") 
COMMENT                       #512=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279128 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Hpy99I: 3 sites") 6 1 "NCGWCGN" 
COMMENT                             "NGCWGCN") 
COMMENT                       #513=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279160 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyAV: 20 sites") 11 10 
COMMENT                             "CCTTCNNNNNNN" "GGAAGNNNNNNN") 
COMMENT                       #514=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279192 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyCH4I: 24 sites") 3 1 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #515=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279224 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyCH4III: 13 sites") 3 2 "ACNGT" 
COMMENT                             "TGNCA") 
COMMENT                       #516=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279400 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyCH4IV: 9 sites") 1 3 "ACGT" 
COMMENT                             "TGCA") 
COMMENT                       #517=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279448 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyCH4V: 36 sites") 2 2 "TGCA" 
COMMENT                             "ACGT") 
COMMENT                       #518=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279488 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyF10VI: 45 sites") 8 3 
COMMENT                             "GCNNNNNNNGC" "CGNNNNNNNCG") 
COMMENT                       #519=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #520=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #164# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #521=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #164# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3391 " 1))))) 
COMMENT                              "KasI: 1 site") 1 5 "GGCGCC" "CCGCGG") 
COMMENT                       #522=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279568 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #523=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #165# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #524=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #165# 1) 1 2 2 0 
COMMENT                                              191) " N1: 495 " 1))))) 
COMMENT                              "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") 
COMMENT                       #525=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279600 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "LpnI: 3 sites") 3 3 "RGCGCY" 
COMMENT                             "YCGCGR") 
COMMENT                       #526=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279632 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MaeIII: 18 sites") 1 6 "NGTNACN" 
COMMENT                             "NCANTGN") 
COMMENT                       #527=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279664 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MboI: 14 sites") 1 5 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #528=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279696 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MboII: 17 sites") 13 12 
COMMENT                             "GAAGANNNNNNNNN" "CTTCTNNNNNNNNN") 
COMMENT                       #529=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279728 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MfeI: 0 sites") 1 5 "CAATTG" 
COMMENT                             "GTTAAC") 
COMMENT                       #530=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279896 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MluI: 0 sites") 1 5 "ACGCGT" 
COMMENT                             "TGCGCA") 
COMMENT                       #531=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279928 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MlyI: 11 sites") 10 10 
COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
COMMENT                       #532=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279960 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MmeI: 9 sites") 26 24 
COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
COMMENT                             "AGGYTGNNNNNNNNNNNNNNNNNNNNN") 
COMMENT                       #533=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34279992 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MnlI: 70 sites") 11 10 
COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
COMMENT                       #534=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34280024 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #535=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #175# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #536=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #175# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2561 " 1))))) 
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COMMENT                       #546=(CRSFolderPar 
COMMENT                             (CFolderPar 
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COMMENT                               (CObjectList)) "NcoI: 2 sites") 1 5 "CCATGG" 
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COMMENT                              "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") 
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COMMENT                              "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") 
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COMMENT                              "PciI: 1 site") 1 5 "ACATGT" "TGTACA") 
COMMENT                       #565=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 34280984 (8 0) 1 1 1 0 178) 
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COMMENT                             "CCANNNNNTGG" "GGTNNNNNACC") 
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COMMENT                               (CObjectList)) "PleI: 11 sites") 9 10 
COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
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COMMENT                                             " N1: 2366 (on complementary strand)" 
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COMMENT                             "GAACNNNNNCTCNNNNNNNNNNNNNN" 
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COMMENT                             "GAACNNNNNNTACNNNNNNNNNNNNN" 
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COMMENT                              "SacI: 1 site") 5 1 "GAGCTC" "CTCGAG") 
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COMMENT                             "CCCGNNNNNNNNN" "GGGCNNNNNNNNN") 
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COMMENT                             "GGATGNNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNNN") 
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FEATURES             Location/Qualifiers
     misc_recomb     358..457
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     misc_recomb     complement(3534..3633)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     CDS             3756..4565
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      4629..5302
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     CDS             505..3519
                     /vntifkey="4"
                     /label=hCARMA2
     misc_feature    853..855
                     /vntifkey="21"
                     /label=G117
     misc_feature    916..918
                     /vntifkey="21"
                     /label=E138
     misc_feature    928..930
                     /vntifkey="21"
                     /label=E142
     misc_feature    1015..1017
                     /vntifkey="21"
                     /label=H171
BASE COUNT     1222 a      1418 c      1566 g      1099 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatccg gtaccgaatt caccatgggg gaactgtgcc gcagggactc cgcactcacg 
      541 gcactggacg aggagacact gtgggagatg atggagagcc accgccacag gatcgtacgc 
      601 tgcatctgcc ccagccgcct caccccctac ctgcgccagg ccaaggtgct gtgccagctg 
      661 gacgaggagg aggtgctgca cagcccccgg ctcaccaaca gcgccatgcg ggccgggcac 
      721 ttgctggatt tgctgaagac tcgagggaag aacggggcca tcgccttcct ggagagcctg 
      781 aagttccaca accctgacgt ctacaccctg gtcaccgggc tgcagcctga tgttgacttc 
      841 agtaacttta gcggtctcat ggagacatcc aagctgaccg agtgcctggc tggggccatc 
      901 ggcagcctgc aggaggagct gaaccaggaa aaggggcaga aggaggtgct gctgcggcgg 
      961 tgccagcagc tgcaggagca cctgggcctg gccgagaccc gtgccgaggg cctgcaccag 
     1021 ctggaggctg accacagccg catgaagcgt gaggttagcg cacacttcca tgaggtgctg 
     1081 aggctgaagg acgagatgct cagcctctcg ctgcactata gcaatgcgct gcaggagaag 
     1141 gagctggccg cctcacgctg ccgcagcctg caggaggagc tgtatctact gaagcaggag 
     1201 ctgcagcgag ccaacatggt ttcctcctgt gagctggaat tgcaagagca gtccctgagg 
     1261 acagccagcg accaggagtc cggggatgag gagctgaacc gcctgaagga ggagaatgag 
     1321 aaactgcgct cgctgacttt cagcctggcg gagaaggaca ttctggagca gagcctggac 
     1381 gaggcgcggg ggagccgaca ggagctggtg gagcgcatcc actcgctgcg ggagcgggcc 
     1441 gtggctgccg agaggcagcg agagcagtac tgggaagaga aggaacagac cctgctgcag 
     1501 ttccagaaga gtaagatggc ctgccaactc tacagggaga aggtgaatgc gctgcaggcc 
     1561 caggtgtgcg agctgcagaa ggagcgagac caggcgtact ccgcgaggga cagtgctcag 
     1621 agggagattt cccagagcct ggtggagaag gactccctcc gcaggcaggt gttcgagctg 
     1681 acggaccagg tctgcgagct gcgcacacag cttcgccagc tgcaggcaga gcctccgggt 
     1741 gtgctcaagc aggaagccag gaccagggag ccctgtccac gggagaagca gcggctggtg 
     1801 cggatgcatg ccatctgccc cagagacgac agcgactgca gcctcgtcag ctccacagag 
     1861 tctcagctct tgtcggacct gagtgccacg tccagccgcg agctggtgga cagcttccgc 
     1921 tccagcagcc ccgcgccccc cagccagcag tccctgtaca agcgggtggc cgaggacttc 
     1981 ggggaagaac cctggtcttt cagcagctgc ctggagatcc cggagggaga cccgggagcc 
     2041 ctgccgggag ctaaggcagg cgacccacac ctggattatg agctcctaga cacggcagac 
     2101 cttccgcagc tggaaagcag cctgcagcca gtctcccctg gaaggcttga tgtctcggag 
     2161 agcggcgtcc tcatgcggcg gaggccagcc cgcaggatcc tgagccaggt caccatgctg 
     2221 gcgttccagg gggatgcatt gctggagcag atcagcgtca tcggcgggaa cctcacgggc 
     2281 atcttcatcc accgggtcac cccgggctcg gcggcggacc agatggcctt gcgcccgggc 
     2341 acccagattg tgatggttga ttacgaagcc tcagagccct tgttcaaggc agtcctggag 
     2401 gacacgaccc tggaggaggc cgtggggctt ctcaggaggg tggacggctt ctgctgcctg 
     2461 tctgtgaagg tcaacacgga cggttataag aggctactcc aggacctgga ggccaaagtg 
     2521 gcgacctcgg gggactcatt ctacatccgg gtcaacctgg ccatggaggg cagggccaaa 
     2581 ggggagctgc aggtgcattg caacgaggtc ctgcacgtca ccgacaccat gttccagggc 
     2641 tgcggctgct ggcatgccca ccgcgtgaac tcttacacca tgaaggatac tgccgcgcac 
     2701 ggcaccatcc ccaactactc cagggctcag cagcagctca tagccctcat ccaggacatg 
     2761 actcagcagt gcaccgtgac ccgcaagcca tcttctgggg gaccacagaa gctggtccgc 
     2821 atcgtcagta tggacaaagc caaggccagc cctctgcgtt tgtcctttga caggggccag 
     2881 ttggacccca gcaggatgga gggctccagc acgtgcttct gggccgagag ctgcctcacc 
     2941 ctggtgccct ataccctggt gcggccccat cgacccgccc ggccccggcc tgtgctcctc 
     3001 gtgcccaggg cggttgggaa gatcctgagc gagaaactgt gcctcctcca agggtttaag 
     3061 aagtgcctgg cagagtactt gagccaggag gagtatgagg cctggagcca gagaggggac 
     3121 atcatccagg agggagaggt gtccgggggc cgctgctggg tgacccgcca tgctgtggag 
     3181 tccctcatgg aaaagaacac ccatgccctc ctggacgtcc agctggacag tgtctgcacc 
     3241 ctgcacagga tggacatctt ccccatcgtc atccacgtct ctgtcaacga gaagatggca 
     3301 aagaagctca agaagggcct acagcggttg ggcacctcag aggagcagct cctggaggct 
     3361 gcgaggcagg aggagggaga cctggaccgg gcgccctgtc tatacagcag cctggctcct 
     3421 gacggctgga gcgacctgga cggcctgctc agctgtgtcc gccaggccat cgccgacgag 
     3481 cagaagaagg tggtgtggac ggagcagagc ccccgatgac tcgagatatc tagacccagc 
     3541 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca 
     3601 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc tggcccgtgt 
     3661 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac 
     3721 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 
     3781 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 
     3841 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag 
     3901 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 
     3961 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 
     4021 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag 
     4081 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 
     4141 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 
     4201 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 
     4261 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 
     4321 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 
     4381 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 
     4441 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 
     4501 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 
     4561 tctaatcaga attggttaat tggttgtaac attattcaga ttgggccccg ttccactgag 
     4621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     4681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     4741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     4801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     4861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     4921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     4981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     5041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     5101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     5161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     5221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     5281 tttgctggcc ttttgctcac atgtt 
//

