LOCUS       pFAJ1700               10533 bp    DNA     circular     27-JUL-2000
DEFINITION  LMBP 9762.
SOURCE      
  ORGANISM  
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     VNTNAME|pFAJ1700|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                          "old + EcoRI-AscI linker (BD38-39)." 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6444..7094)
                     /vntifkey="4"
                     /label=tetR
     CDS             7200..8399
                     /vntifkey="4"
                     /label=tetA
     misc_difference complement(10064..10174)
                     /vntifkey="85"
                     /label=oriT
     rep_origin      complement(5321..5853)
                     /vntifkey="33"
                     /label=oriV
     CDS             complement(3433..4581)
                     /vntifkey="4"
                     /label=trfA
     CDS             complement(8918..9778)
                     /vntifkey="4"
                     /label=bla
     terminator      complement(91..118)
                     /vntifkey="43"
                     /label=T
     terminator      10506..10533
                     /vntifkey="43"
                     /label=T
     CDS             complement(813..1064)
                     /vntifkey="4"
                     /label=parD
     CDS             complement(505..816)
                     /vntifkey="4"
                     /label=parE
     CDS             1215..1508
                     /vntifkey="4"
                     /label=parC
     CDS             1493..2038
                     /vntifkey="4"
                     /label=parB
     CDS             1963..2658
                     /vntifkey="4"
                     /label=parA
     misc_feature    7..87
                     /vntifkey="21"
                     /label=MCS
BASE COUNT     2041 a      3196 c      3162 g      2133 t         1 others
ORIGIN
        1 ggcgcgaagc tttctagagg atccggcgcg ccgcggcacg cgttaacggc cggccgttta 
       61 aacctgcagg aattcgagct cggtaccaac aaaaaaaagc ccgctcatta ggcgggcttg 
      121 atcccccgat gtgtagcagt ggcggaccat ataggcagat cagaaggcgc ggttctccta 
      181 catgttcgcc ggtgaacgcg ttgaggaagc cgggcagtgc ctcggcaaaa tccttgcgtg 
      241 tagacaagac atctgcgtag cagttgtcct caacaacgat gtcgaaatcc aaatcggagt 
      301 gctcatcgag tcctccgtga acgtaagagc cgccgatcag aagagcgcgg aagcgaacat 
      361 cggaagcgac cgcatcgcgg atgcggttca agaaagttgc atgagcttgt ggaagtgtgc 
      421 tgagcataaa tgattctcct agctgttctt tgggtaagta cgccatcagg acgttgtgag 
      481 tggcgcgatt tttagcggct gaaatcagcc cttgagcctg tcggcaagtc gcgtcatgag 
      541 gtccatgcgc tcatgcagga tcgccacgac caacgcgggt tcgcccgcac gcggcaggca 
      601 aaaaacgtag tggtgttcgc agcgggccat ccgcagcgcg ggaaagagtt cgctcatgtc 
      661 cttaaacggg ccttcgccgg cggcaagcct ggctatgccc tgttccagct tagcgatata 
      721 gcggcgcacc tgcgccgcgc cccactcccg gcgcgtgtag cggatgatgc cgcgtagatc 
      781 ggcttcggcc tcagccgtga ggatgtaggc cgtcaagcgc gatccccgct gagttcttca 
      841 tcaagaattt cgccgacgct cttggtggac accttgccgg caagcccatc gttgatgcgg 
      901 ttccccagca tggttttcag ttcctgccat gcctgatcgg catcagcgtc accggggaac 
      961 agacgttcga gggcgtattg cttaatggtc ttgccctgca aggcggccag ggctttcagg 
     1021 ctctggtgct gctggtccgt catgtcgatt gtcaggcggc tcattggata acctccataa 
     1081 aatacacgta accacattag cacatatgtg ggcgtgaggc tacagcgcga ggcgcattaa 
     1141 ggtcgggaaa atgcgctagg cgcatttaaa ttgcgtattg ctgtaatgcg ccatgccggc 
     1201 tagactaggc ccaaatgggt atacccaatt tgaccaaggg ggacgcgatg agggcggcca 
     1261 agcactaccg acaacttcta tccatcgact tcaacatcga ggcgctggcc ttcgtgcctg 
     1321 gacccgacgg cacacgcggc cggcgcatcc acgtcctggg gcgcgaggtc cgcgaccggc 
     1381 ccggcctggt cgagtacctt tcgccggcgt tcggctcgcg ggtggcgctg gacggctact 
     1441 gcaaggccaa tttcgatgca gtgctgcacc tggcgtaccc cgatcatcag caatggggcc 
     1501 acgcatgaag cgccgaagct acgccatgct gcgcgccgct gccgcgctgg ccgtcctggt 
     1561 cgttgcctcg ccggcatggg ccgagctgcg cggcgaggtc gtgcgcatca tcgacggcga 
     1621 caccatcgac gtgctggtag acaagcagcc ggtgcgcgtg cgcctggtgg acattgacgc 
     1681 gccggaaaag cggcaagcct tcggcgaacg tgcgcgccag gcgctggccg gcatggtgtt 
     1741 ccgccggcac gtcctggtcg acgagaagga caccgaccgt tacggccgca cgctgggcac 
     1801 cgtgtgggtc aacatggagc tggccagccg gccgccgcag ccgcgcaacg tcaacgccgc 
     1861 gatggttcac cagggcatgg cgtgggccta tcgcttccac ggccgcgcgg ccgaccctga 
     1921 aatgctgcgg ctcgaacagg aggcgcgagg caagcgcgtc ggcctctggt ccgatccgca 
     1981 cgccgtcgag ccgtggaaat ggcgacgcga gagcaacaac cggagggacg aaggttgaag 
     2041 gtcgcccgca tctacctgcg cgccagtacg gacgagcaga atcttgaacg ccaggagagc 
     2101 cttgtagcgg ccacgcgggc cgccgggtac tacgtcgccg gcatctaccg cgagaaggcg 
     2161 tccggcgcac gcgccgaccg gcccgagctg ctgcgcatga tcgcggacct gcaacctggt 
     2221 gaagtcgtcg ttgcggagaa gatcgaccgc atcagccgct tgccgttggc cgaggccgag 
     2281 cgcctggttg cgtcgatccg ggccaaaggg gccaagctgg ccgtgcctgg cgtggtggac 
     2341 ctgtcggagc tggccgccga ggcgaacgga gtggcgaaaa tcgttctgga atccgtccag 
     2401 gacatgcttt tgaagctcgc cttgcagatg gcccgcgacg actacgagga tcggcgcgag 
     2461 cgtcaacgtc agggtgtcca gttggcgaag gccgccggcc gctacaccgg ccgcaaacgt 
     2521 gacgccggca tgcacgaccg catcatcacg cttcgctccg gcggatcgag cattgccaag 
     2581 acggccaagc tggtcggatg cagcccgagc caggtcaaac gagtgtgggc ggcctggaac 
     2641 gcgcagcagc aaaaataaag ccgggcagtg cccggctttt ctcacctttt cgcgtcccgc 
     2701 agggccgctg cgagcgccct acctagatcc tcgctttccc cctcggtgta gtccggccag 
     2761 ggcacgaagg gcgcggatgc gaacctgttg agcaggtacg ccttcgggca gcggtagacc 
     2821 accggcgagt tcgccttttc atcccaccgg gccaggatca cgtccgcatc gcagtgcatg 
     2881 tccttcacct ggtcgcggaa gaagccgaag gccaccatgc cgctatgttc gccgaggaac 
     2941 gccagttgct tcgcgctggc gatcgcgccg acgccgccgg ccaaaaccga cgccatcacc 
     3001 cagccgacga accagaagct ggcatgcttg cggttgacca ccgcacgcgc agccgcgacc 
     3061 aggacaacgg ccaagctgcc gaccagggcc atgacgaccg tgatccggcc gttgtggaaa 
     3121 gcgatgggct tgccagcgtc cgcttgcacg gcgtcgtaaa tgctggaccc gatgggcgcg 
     3181 cacatcagca cgacaggcag cagcaccagg aacatcgtcc gcgtccattg cgcgagtgcc 
     3241 ttgcggcgtt cgccggcggc aagcgcctcc atcatcggcg tgaagcccaa cagggccacc 
     3301 gcagccgcca agccggcaac gatgccgcag gcgattacat acatacatcc tccctaatgc 
     3361 gccttgcgca cggttgtagt cagagtccgg ggtggctgct gaacccccag ccggaactga 
     3421 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac 
     3481 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac 
     3541 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc 
     3601 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt 
     3661 ctcccatatg aatttcgtgt agtggtcgcc agcaaactgc acgacgattt cctcgtgcat 
     3721 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag 
     3781 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag 
     3841 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc 
     3901 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta 
     3961 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta 
     4021 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg 
     4081 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg 
     4141 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg 
     4201 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc 
     4261 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt 
     4321 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc 
     4381 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg 
     4441 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc 
     4501 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt 
     4561 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccggaat taattccccg 
     4621 gatcgatccg tcgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc 
     4681 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt 
     4741 tcgcttgctg gccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct 
     4801 aggtcgtttg tgtttgcgct tgcgttttcc cttgtccaga tagcccagta cgtgacattc 
     4861 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag 
     4921 cccttgcgcc ctgagtgctt gcggcagcgt gaagctgacg gatcgatccg gggaattaat 
     4981 tcacccccga acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa 
     5041 attgcccgcc ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg 
     5101 ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc 
     5161 tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc 
     5221 ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg 
     5281 gccgaagggc gcagccgggg ggatgggagg cccgcgttag ccgggagggt tcgagaaggg 
     5341 ggggcacccc ccttcggcgt gcgcggtcac gcgccagggc gcagccctgg ttaaaaacaa 
     5401 ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg 
     5461 ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata 
     5521 ggtgcgcccc tcatctgtca tcactctgcc cctcaagtgt caaggatcgc gcccctcatc 
     5581 tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc 
     5641 acatcatgtc tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc 
     5701 agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg 
     5761 agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg 
     5821 cgtggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg 
     5881 gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga 
     5941 gcgtcggaaa ggcgctggaa gccccgtagc gacgcggaga ggggcgagac aagccaaggg 
     6001 cgcaggcntc gagtcgcagc acgacatagc cggttctgcg caaggacgag aatttccctg 
     6061 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt 
     6121 ccacgctaga tccgtcaatt cgatctatct catctgcgca aggcagaacg tgaagacggc 
     6181 cgccctggac ctcgcccgcg agcgccaggc gcacgaggcc ggcgcgcgga cccgcgccac 
     6241 ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg 
     6301 ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg 
     6361 tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt agtgagtggg 
     6421 ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc aacgttcctg 
     6481 acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct 
     6541 gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga 
     6601 gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc 
     6661 acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa 
     6721 aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc 
     6781 ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag 
     6841 ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc 
     6901 gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg 
     6961 aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc 
     7021 agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc 
     7081 tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat 
     7141 cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagacg 
     7201 tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca 
     7261 tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg 
     7321 tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt gcctgcgcac 
     7381 ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc gtctcgctgg 
     7441 ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg gttctctata 
     7501 tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg 
     7561 ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg 
     7621 ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg 
     7681 ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt 
     7741 tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg 
     7801 cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca 
     7861 tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct 
     7921 ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac 
     7981 tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca 
     8041 tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat 
     8101 ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc 
     8161 aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg 
     8221 cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg 
     8281 cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct 
     8341 gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc gatcgctgat 
     8401 cgtggaaacg atagggaatt gacggatcta tctcatctgc gcaaggcaga acgtgaagac 
     8461 ggccgccttg gacctcgccc gcgagcgcca gcgcacgagg ccggcgcgcg gacccgccgc 
     8521 ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg 
     8581 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagggggcgc tacggggctc 
     8641 tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt 
     8701 gcgctccggg agcggtcctg atcccccgca gaaaaaaagg atctcaagaa gatcctttga 
     8761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     8821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     8881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     8941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     9001 agataactac gatacgggag ggcttaccat ccggccccag tgctgcaatg ataccgcgag 
     9061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     9121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     9181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     9241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     9301 ggcgtgttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     9361 tcgttgtcag aagtaagttg gccgcagtgt tatcagcgat ggttatggca gcactgcata 
     9421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     9481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg 
     9541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     9601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     9661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     9721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     9781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     9841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     9901 tgccacctga cgtggtcgac tgctttccgc tgctataacc ctgcttcggg gtcattatag 
     9961 cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc 
    10021 gtgtagactt tccttggtgt atccaacggc gtcagccggg cagccgggca ggataggtga 
    10081 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt 
    10141 gctcaacggg aatcctgctc tgcgaggctg gccgtattcg actaagaaac cattattatc 
    10201 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt 
    10261 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
    10321 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 
    10381 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 
    10441 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgggc 
    10501 gcgttagccc gcctaatgag cgggcttttt ttt 
//
