LOCUS       pEGFP-fliC-1-52        4844 bp    DNA     circular     22-JAN-2016
DEFINITION  LMBP 9780.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11074688|
COMMENT     VNTNAME|pEGFP-fliC-1-52|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 1 1 1 1 "5871..6197") 
COMMENT        #2=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 1 568 0 
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COMMENT        #3=(CFSignal (CObList) "EGFP" 4 0 0 613 1329 0 (CStringList) 
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COMMENT        #4=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1610 1706 0 
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COMMENT                                   13.5909 10.454 0 -10 13.065 1.16519 #162#) 
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FEATURES             Location/Qualifiers
     terminator      3725..3794
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      4512..4838
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        1..568
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             613..1329
                     /vntifkey="4"
                     /label=EGFP
     terminator      1610..1706
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1610..1706)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1760..2215)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        2216..2311
                     /vntifkey="30"
                     /label=amp\promoter
     RBS             2312..2346
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        2367..2648
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      2605..2680
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             2740..3534
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             1369..1527
                     /vntifkey="4"
                     /label=S.\enterica\fliC\fragment
BASE COUNT     1171 a      1316 c      1268 g      1089 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattcat ggcacaagtc 
     1381 attaatacaa acagcctgtc gctgttgacc cagaataacc tgaacaaatc ccagtccgct 
     1441 ctgggcaccg ctatcgagcg tctgtcttcc ggtctgcgta tcaacagcgc gaaagacgat 
     1501 gcggcaggtc aggcgattgc taactgatca taatcagcca taccacattt gtagaggttt 
     1561 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 
     1621 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1681 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     1741 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 
     1801 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 
     1861 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 
     1921 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 
     1981 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 
     2041 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 
     2101 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 
     2161 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt 
     2221 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     2281 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     2341 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt 
     2401 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     2461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     2521 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     2581 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     2641 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 
     2701 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 
     2761 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 
     2821 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 
     2881 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 
     2941 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 
     3001 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 
     3061 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 
     3121 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 
     3181 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 
     3241 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 
     3301 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 
     3361 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 
     3421 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 
     3481 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 
     3541 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 
     3601 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 
     3661 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa 
     3721 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 
     3781 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 
     3841 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 
     3901 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 
     3961 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc 
     4021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     4081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     4141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     4201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     4261 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 
     4321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     4381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     4441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     4501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     4561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     4621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     4681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     4741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 
     4801 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat 
//
