LOCUS       pEGFP-hIKKe-K38A        6858 bp    DNA     circular      9-MAR-2016
DEFINITION  LMBP 9790.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9790
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
COMMENT     VNTDATE|-11142221|
COMMENT     VNTNAME|pEGFP-hIKKe-K38A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEGFP-hIKKe-K38A" 0 6858 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 92 91 1 6858 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "hCMV promoter" 30 0 0 1 568 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1..568") 
COMMENT        #1=(CFSignal (CObList) "EGFP" 4 0 0 613 1329 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "613..1329") 
COMMENT        #2=(CFSignal (CObList) "SV40 early promoter" 30 0 0 4381 4662 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2254..2535") 
COMMENT        #3=(CFSignal (CObList) "f1 ori" 33 0 1 3774 4229 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1647..2102)") 
COMMENT        #4=(CFSignal (CObList) "pMB1 ori" 33 0 1 6526 6852 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4399..4725)") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3624 3720 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1497..1593") 
COMMENT        #6=(CFSignal (CObList) "TK polyA" 43 0 0 5739 5808 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3612..3681") 
COMMENT        #7=(CFSignal (CObList) "ampicillin promoter" 30 0 0 4230 4325 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2103..2198") 
COMMENT        #8=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4754 5548 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2627..3421") 
COMMENT        #9=(CFSignal (CObList) "SV40 ori" 33 0 0 4381 4721 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2254..2594") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3624 3720 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1497..1593)") 
COMMENT        #11=(CFSignal (CObList) "hIKBKE mutant" 4 0 0 1345 3495 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "K38A" 62 0 0 1456 1458 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pEGFP-hIKKe-K38A'" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9790." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/09/16 01:56" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/09/16 01:56" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6858 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-SEP-2013" 1))) 
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COMMENT                                        "Start: 613   End: 1329" 1) 
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COMMENT                                        "enhanced green fluorescent protein gene" 
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FEATURES             Location/Qualifiers
     promoter        1..568
                     /vntifkey="30"
                     /label=hCMV\promoter
     CDS             613..1329
                     /vntifkey="4"
                     /label=EGFP
                     /note="enhanced green fluorescent protein gene"
     promoter        4381..4662
                     /vntifkey="30"
                     /label=SV40\early\promoter
                     /note="Simian virus 40 early promoter (SV40 early)"
     rep_origin      complement(3774..4229)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(6526..6852)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      3624..3720
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      5739..5808
                     /vntifkey="43"
                     /label=TK\polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)"
     promoter        4230..4325
                     /vntifkey="30"
                     /label=ampicillin\promoter
     CDS             4754..5548
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
                     /note="Tn5 antibiotic resistance gene"
     rep_origin      4381..4721
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      complement(3624..3720)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             1345..3495
                     /vntifkey="4"
                     /label=hIKBKE\mutant
     mutation        1456..1458
                     /vntifkey="62"
                     /label=K38A
                     /note="WT: AAG"
BASE COUNT     1613 a      1890 c      1905 g      1450 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggactcag atctatgcag agcacagcca attacctgtg gcacacagat 
     1381 gacctgctgg ggcagggggc cactgccagt gtgtacaagg cccgcaacaa gaaatccgga 
     1441 gagctggttg ctgtggcggt cttcaacact accagctacc tgcggccccg cgaggtgcag 
     1501 gtgagggagt ttgaggtcct gcggaagctg aaccaccaga acatcgtcaa gctctttgcg 
     1561 gtggaggaga cgggcggaag ccggcagaag gtactggtga tggagtactg ctccagtggg 
     1621 agcctgctga gtgtgctgga gagccctgag aatgcctttg ggctgcctga ggatgagttc 
     1681 ctggtggtgc tgcgctgtgt ggtggccggc atgaaccacc tgcgggagaa cggcattgtg 
     1741 catcgcgaca tcaagccggg gaacatcatg cgcctcgtag gggaggaggg gcagagcatc 
     1801 tacaagctga cagacttcgg cgctgcccgg gagctggatg atgatgagaa gttcgtctcg 
     1861 gtctatggga ctgaggagta cctgcatccc gacatgtatg agcgggcggt gcttcgaaag 
     1921 ccccagcaaa aagcgttcgg ggtgactgtg gatctctgga gcattggagt gaccttgtac 
     1981 catgcagcca ctggcagcct gcccttcatc ccctttggtg ggccacggcg gaacaaggag 
     2041 atcatgtacc ggatcaccac agagaagccg gctggggcca ttgcaggtgc ccagaggcgg 
     2101 gagaacgggc ccctggagtg gagctacacc ctccccatca cctgccagct gtcactgggg 
     2161 ctgcagagcc agctggtgcc catcctggcc aacatcctgg aggtggagca ggccaagtgc 
     2221 tggggcttcg accagttctt tgcggagacc agtgacatcc tgcagcgagt tgtcgtccat 
     2281 gtcttctccc tgtcccaggc agtcctgcac cacatctata tccatgccca caacacgata 
     2341 gccattttcc aggaggccgt gcacaagcag accagtgtgg ccccccgaca ccaggagtac 
     2401 ctctttgagg gtcacctctg tgtcctcgag cccagcgtct cagcacagca catcgcccac 
     2461 acgacggcaa gcagccccct gaccctcttc agcacagcca tccctaaggg gctggccttc 
     2521 agggaccctg ctctggacgt ccccaagttc gtccccaaag tggacctgca ggcggattac 
     2581 aacactgcca agggcgtgtt gggcgccggc taccaggccc tgcggctggc acgggccctg 
     2641 ctggatgggc aggagctaat gtttcggggg ctgcactggg tcatggaggt gctccaggcc 
     2701 acatgcagac ggactctgga agtggcaagg acatccctcc tctacctcag cagcagcctg 
     2761 ggaactgaga ggttcagcag cgtggctgga acgcctgaga tccaggaact gaaggcggct 
     2821 gcagaactga ggtccaggct gcggactcta gcggaggtcc tctccagatg ctcccaaaat 
     2881 atcacggaga cccaggagag cctgagcagc ctgaaccggg agctggtgaa gagccgggat 
     2941 caggtacatg aggacagaag catccagcag attcagtgct gtttggacaa gatgaacttc 
     3001 atctacaaac agttcaagaa gtctaggatg aggccagggc ttggctacaa cgaggagcag 
     3061 attcacaagc tggataaggt gaatttcagt catttagcca aaagactcct gcaggtgttc 
     3121 caggaggagt gcgtgcagaa gtatcaagcg tccttagtca cacacggcaa gaggatgagg 
     3181 gtggtgcacg agaccaggaa ccacctgcgc ctggttggct gttctgtggc tgcctgtaac 
     3241 acagaagccc agggggtcca ggagagtctc agcaagctcc tggaagagct atctcaccag 
     3301 ctccttcagg accgagcaaa gggggctcag gcctcgccgc ctcccatagc tccttacccc 
     3361 agccctacac gaaaggacct gcttctccac atgcaagagc tctgcgaggg gatgaagctg 
     3421 ctggcatctg acctcctgga caacaaccgc atcatcgaac ggctaaatag agtcccagca 
     3481 cctcctgatg tctgagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg 
     3541 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 
     3601 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 
     3661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     3721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt 
     3781 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 
     3841 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 
     3901 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 
     3961 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 
     4021 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 
     4081 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 
     4141 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 
     4201 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 
     4261 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 
     4321 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag 
     4381 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 
     4441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
     4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
     4561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     4621 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     4681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg 
     4741 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 
     4801 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 
     4861 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 
     4921 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 
     4981 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 
     5041 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 
     5101 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 
     5161 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 
     5221 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 
     5281 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 
     5341 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 
     5401 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 
     5461 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 
     5521 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 
     5581 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 
     5641 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 
     5701 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg 
     5761 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg 
     5821 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga 
     5881 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg 
     5941 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt 
     6001 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     6061 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     6121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     6181 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     6241 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     6301 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     6361 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     6421 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     6481 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     6541 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     6601 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     6661 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     6721 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     6781 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     6841 cgtattaccg ccatgcat 
//
