LOCUS       pEGFP-hTANK              6003 bp    DNA     circular      9-MAR-2016
DEFINITION  LMBP 9791.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9791
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
COMMENT     VNTDATE|-11142239|
COMMENT     VNTNAME|pEGFP-hTANK|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEGFP-hTANK" 0 6003 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 5789 5791 1 6003 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 1 1 1 1 "1..568") 
COMMENT        #1=(CFSignal (CObList) "EGFP" 4 0 0 613 1329 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "613..1329") 
COMMENT        #2=(CFSignal (CObList) "SV40 early promoter" 30 0 0 3526 3807 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2254..2535") 
COMMENT        #3=(CFSignal (CObList) "f1 ori" 33 0 1 2919 3374 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1647..2102)") 
COMMENT        #4=(CFSignal (CObList) "pMB1 ori" 33 0 1 5671 5997 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4399..4725)") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2769 2865 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1497..1593") 
COMMENT        #6=(CFSignal (CObList) "TK polyA" 43 0 0 4884 4953 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3612..3681") 
COMMENT        #7=(CFSignal (CObList) "ampicillin promoter" 30 0 0 3375 3470 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2103..2198") 
COMMENT        #8=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 3899 4693 0 
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COMMENT        #9=(CFSignal (CObList) "SV40 ori" 33 0 0 3526 3866 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "hTANK" 4 0 0 1396 2673 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pEGFP-hTANK'" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9791." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/09/16 02:14" 1) 
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COMMENT                          "Last Modified: 03/09/16 02:14" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6003 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-SEP-2013" 1))) 
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FEATURES             Location/Qualifiers
     promoter        1..568
                     /vntifkey="30"
                     /label=hCMV\promoter
     CDS             613..1329
                     /vntifkey="4"
                     /label=EGFP
                     /note="enhanced green fluorescent protein gene"
     promoter        3526..3807
                     /vntifkey="30"
                     /label=SV40\early\promoter
                     /note="Simian virus 40 early promoter (SV40 early)"
     rep_origin      complement(2919..3374)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(5671..5997)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      2769..2865
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      4884..4953
                     /vntifkey="43"
                     /label=TK\polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)"
     promoter        3375..3470
                     /vntifkey="30"
                     /label=ampicillin\promoter
     CDS             3899..4693
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
                     /note="Tn5 antibiotic resistance gene"
     rep_origin      3526..3866
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      complement(2769..2865)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             1396..2673
                     /vntifkey="4"
                     /label=hTANK
BASE COUNT     1576 a      1570 c      1492 g      1365 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 
     1381 gcgggcccgg gatccatgga taaaaacatt ggcgagcaac tcaataaagc gtatgaagcc 
     1441 ttccggcagg catgcatgga tagagattct gcagtaaaag aattacagca aaagactgag 
     1501 aactatgagc agagaatacg tgaacaacag gaacagctgt cactccaaca gactattatt 
     1561 gacaagctaa aatctcagtt acttcttgtg aattccactc aagataacaa ttatggctgt 
     1621 gtccctctgc ttgaagacag tgacacaaga aagaatactt tgactcttgc tcagccacaa 
     1681 gataaagtga tttcaggaat agcaagagaa aaactaccaa aggtaagaag acaagaggtt 
     1741 tcttctccta gaaaagaaac ttcagcaagg agtcttggca gtcctttgct ccatgaaagg 
     1801 ggtaatatag agaagacttc ctgggatctg aaagaagaat ttcataaaat atgcatgcta 
     1861 gcaaaagcac agaaagacca cttaagcaaa cttaatatac cagacactgc aactgaaaca 
     1921 cagtgctctg tgcctataca gtgtacggat aaaacagata aacaagaagc gctgtttacg 
     1981 cctcaggcta aagatgatat aaatagaggt gcaccatcca tcacatctgt cacaccaaga 
     2041 ggactgtgca gagatgagga agacacctct ttggaatcac tttctaaatt caatgtcaag 
     2101 tttccaccta tggacaatga ctcaactttc ttacatagca ctccagagag acccggcatc 
     2161 cttagtcctg ccacgtctga ggcagtgtgc caagagaaat ttaatatgga gttcagagac 
     2221 aacccaggga actttgttaa aacagaagaa actttatttg aaattcaggg aattgacccc 
     2281 atagcttcag ctatacaaaa ccttaaaaca actgacaaaa caaagccctc aaatctcgta 
     2341 aacacttgta tcaggacaac tctggataga gctgcgtgtt tgccacctgg agaccataat 
     2401 gcattatatg taaatagctt cccacttctg gacccatctg atgcaccttt tccctcactc 
     2461 gattccccgg gaaaagcaat ccgaggacca cagcagccca tttggaagcc ctttcctaat 
     2521 caagacagtg actcggtggt actaagtggc acagactcag aactgcatat acctcgagta 
     2581 tgtgaattct gtcaagcagt tttcccacca tccattacat ccagggggga tttccttcgg 
     2641 catcttaatt cacacttcaa tggagagact taatctagat aactgatcat aatcagccat 
     2701 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 
     2761 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 
     2821 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     2881 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 
     2941 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 
     3001 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 
     3061 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 
     3121 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 
     3181 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 
     3241 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 
     3301 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 
     3361 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     3421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     3481 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 
     3541 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     3601 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 
     3661 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 
     3721 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     3781 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     3841 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 
     3901 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     3961 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     4021 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     4081 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     4141 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     4201 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     4261 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     4321 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     4381 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 
     4441 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     4501 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     4561 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     4621 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     4681 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
     4741 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     4801 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     4861 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 
     4921 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 
     4981 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 
     5041 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 
     5101 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 
     5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 
     5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     5941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 
     6001 cat 
//
