LOCUS       pCi-C-TagRFP-DEST      6402 bp    DNA     circular UNA 12-OCT-2022
DEFINITION  LMBP 9841.
COMMENT     Accession number: LMBP 9841
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-OCT-2022
FEATURES             Location/Qualifiers
     promoter        138..721
                     /label=hCMV-IE\promoter\and\enhancer
     intron          857..989
                     /label=chimeric\intron
     promoter        1034..1050
                     /label=T7\promoter
     misc_recomb     complement(1067..1191)
                     /label=attR1
     promoter        1199..1251
                     /label=lacUV5\promoter
     CDS             1300..1959
                     /label=chloramphenicol\resistance
     CDS             2280..2585
                     /label=ccdB
     misc_recomb     2626..2750
                     /label=attR2
     CDS             2782..3474
                     /label=TagRFP
     terminator      complement(3563..3659)
                     /label=SV40\polyA\early
     terminator      3563..3659
                     /label=SV40\polyA\late
     rep_origin      3818..4273
                     /label=f1\ori
     CDS             4710..5570
                     /label=ampicillin\resistance
     rep_origin      5741..6332
                     /label=pMB1\ori
ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC
      661 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC
      721 TCGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG GTAGTTTATC ACAGTTAAAT
      781 TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC
      841 GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA
      901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC
      961 TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA
     1021 AGGCTAGAGT ACTTAATACG ACTCACTATA GGCTAGCCTC GAGATCACAA GTTTGTACAA
     1081 AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA
     1141 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGCGGCCGCA
     1201 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG GATTTTGAGT
     1261 TAGGATCCGT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA
     1321 TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA
     1381 GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC
     1441 GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG
     1501 AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT
     1561 GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT
     1621 GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC
     1681 GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC
     1741 AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC
     1801 GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG
     1861 GCGATTCAGG TTCATCATGC CGTTTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA
     1921 TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAA CGCGTGGATC CGGCTTACTA
     1981 AAAGCCAGAT AACAGTATGC GTATTTGCGC GCTCGCGAAC CGGTGTATAC CCGAAGTATG
     2041 TCAAAAAGAG GTATGCTATG AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC
     2101 AGTTGCTCAA GGCATATATG ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA
     2161 TGAAGCCCGT CGTCTGCGTG CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA
     2221 GGTCGCCCGG TTTATTGAAA TGAACGGCTC TTTTGCTGAC GAGAACAGGG GCTGGTGAAA
     2281 TGCAGTTTAA GGTTTACACC TATAAAAGAG AGAGCCGTTA TCGTCTGTTT GTGGATGTAC
     2341 AGAGTGATAT TATTGACACG CCCGGGCGAC GGATGGTGAT CCCCCTGGCC AGTGCACGTC
     2401 TGCTGTCAGA TAAAGTCTCC CGTGAACTTT ACCCGGTGGT GCATATCGGG GATGAAAGCT
     2461 GGCGCATGAT GACCACCGAT ATGGCCAGTG TGCCGGTCTC CGTTATCGGG GAAGAAGTGG
     2521 CTGATCTCAG CCACCGCGAA AATGACATCA AAAACGCCAT TAACCTGATG TTCTGGGGAA
     2581 TATAAATGTC AGGCTCCCTT ATACACAGCC AGTCTGCAGG TCGACCATAG TGACTGGATA
     2641 TGTTGTGTTT TACAGTATTA TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG
     2701 ATATTTATAT CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACAAAGTGGT TGATCTCGAG
     2761 AACTTCACGC GTGGTACCCC GGAAGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC
     2821 ATGGAGGGCA CCGTGAACAA CCACCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC
     2881 TACGAGGGCA CCCAGACCAT GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC
     2941 TTCGACATCC TGGCTACCAG CTTCATGTAC GGCAGCAGAA CCTTCATCAA CCACACCCAG
     3001 GGCATCCCCG ACTTCTTTAA GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC
     3061 ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC
     3121 CTCATCTACA ACGTCAAGAT CAGAGGGGTG AACTTCCCAT CCAACGGCCC TGTGATGCAG
     3181 AAGAAAACAC TCGGCTGGGA GGCCAACACC GAGATGCTGT ACCCCGCTGA CGGCGGCCTG
     3241 GAAGGCAGAA CCGACATGGC CCTGAAGCTC GTGGGCGGGG GCCACCTGAT CTGCAACTTC
     3301 AAGACCACAT ACAGATCCAA GAAACCCGCT AAGAACCTCA AGATGCCCGG CGTCTACTAT
     3361 GTGGACCACA GACTGGAAAG AATCAAGGAG GCCGACAAAG AGACCTACGT CGAGCAGCAC
     3421 GAGGTGGCTG TGGCCAGATA CTGCGACCTC CCTAGCAAAC TGGGGCACAA ATAGGCTCTA
     3481 GAGTCGACCC GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA
     3541 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
     3601 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT
     3661 TATGTTTCAG GTTCAGGGGG AGATGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA
     3721 ATGTGGTAAA ATCGATAAGG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG
     3781 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG CGGCGCATTA
     3841 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
     3901 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
     3961 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC
     4021 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT
     4081 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA
     4141 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
     4201 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
     4261 ACGCTTACAA TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC
     4321 GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC
     4381 ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA
     4441 GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA
     4501 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA
     4561 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT
     4621 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA
     4681 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA
     4741 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG
     4801 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA
     4861 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA
     4921 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC
     4981 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC
     5041 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA
     5101 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC
     5161 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA
     5221 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC
     5281 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG
     5341 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG
     5401 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG
     5461 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC
     5521 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC
     5581 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT
     5641 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC
     5701 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC
     5761 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
     5821 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA
     5881 ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC
     5941 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT
     6001 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
     6061 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
     6121 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC
     6181 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT
     6241 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT
     6301 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
     6361 TGGCCTTTTG CTGGCCTTTT GCTCACATGG CTCGACAGAT CT
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