LOCUS       pCi-N-TagBFP-DEST         6381 bp    DNA     circular     29-JAN-2016
DEFINITION  LMBP 9842.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11084612|
COMMENT     VNTNAME|pCi-N-TagBFP-DEST|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CMolPar 0 0 1 5869 5868 1 6381 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "attR1" 0 0 1 1781 1905 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1909 1965 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 2014 2673 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "ccdB" 4 0 0 2994 3299 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "attR2" 0 0 0 3340 3464 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "SV40 polyA early" 43 0 1 3540 3636 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 0 6090 6368 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "tagBFP" 4 0 0 1064 1762 0 (CStringList) 
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COMMENT                                   "chloramphenicol resistance" "@N" 1 16.4962 
COMMENT                                   -8.71165 0 -10 11.0073 1.16519 #131#) 
COMMENT                             #133=(CWideArrow 
COMMENT                                   (CWideLine 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
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COMMENT                                   2.4695 -13.9386 0 -10 2.15684 1.16519 #133#) 
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COMMENT                                   "ampicillin resistance" "@N" 1 -17.222 
COMMENT                                   5.22699 0 -10 8.03237 1.16519 #135#) 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.56437 6.1385 1 0.082322) 0.8 1.8 0) 
COMMENT                             #141=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "hCMV-IE promoter and enhancer" "@N" 1 13.469 
COMMENT                                   12.1963 0 -10 13.065 1.16519 #140#)) 
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COMMENT                      #142=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -1030 100) 
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COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #82# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 5.17037 5.25764) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #144=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 14.6867 6.96932 0 -10 4.88388 1.16519 
COMMENT                                   #143#) 
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COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #105# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
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COMMENT                                   "lacUV5 promoter" "@N" 1 16.4396 -5.22699 0 
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COMMENT                                   "SV40 polyA early" "@N" 1 -10.0976 -12.1963 0 
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FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1781..1905)
                     /vntifkey="0"
                     /label=attR1
     promoter        1909..1965
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             2014..2673
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2994..3299
                     /vntifkey="4"
                     /label=ccdB
     attenuator      3340..3464
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(3540..3636)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3540..3636
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3795..4250
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4689..5549
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6090..6368
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1064..1762
                     /vntifkey="4"
                     /label=tagBFP
BASE COUNT     1677 a      1513 c      1540 g      1651 t 
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagctag accatgagcg agctgattaa 
     1081 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg 
     1141 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt 
     1201 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag 
     1261 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga 
     1321 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca 
     1381 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt 
     1441 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac 
     1501 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg 
     1561 cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa 
     1621 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa 
     1681 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag 
     1741 caaactgggg cacaagctta atctctcgag tcgacccatc acaagtttgt acaaaaaagc 
     1801 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa 
     1861 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc 
     1921 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat 
     1981 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc 
     2041 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct 
     2101 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag 
     2161 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct 
     2221 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac 
     2281 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac 
     2341 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa 
     2401 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc 
     2461 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc 
     2521 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt 
     2581 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa 
     2641 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc 
     2701 agataacagt atgcgtattt gcgcgctcgc gaaccggtgt atacccgaag tatgtcaaaa 
     2761 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc 
     2821 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc 
     2881 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc 
     2941 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt 
     3001 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg 
     3061 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt 
     3121 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca 
     3181 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc 
     3241 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa 
     3301 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt 
     3361 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt 
     3421 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatggg cggccgcttc 
     3481 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 
     3541 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 
     3601 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga 
     3661 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc 
     3721 cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 
     3781 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     3841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     3901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     3961 agggttccga tttagagctt tacggcacct cgaccgcaaa aaacttgatt tgggtgatgg 
     4021 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4081 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
     4141 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     4201 ttaacaaata tttaacgcga attttaacaa aatattaacg tttacaattt cgcctgatgc 
     4261 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 
     4321 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 
     4381 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     4441 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 
     4501 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 
     4561 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     4621 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     4681 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     4741 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     4801 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     4861 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     4921 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     4981 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     5041 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     5101 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     5161 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     5221 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     5281 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     5341 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     5401 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     5461 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     5521 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     5581 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     5641 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     5701 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     5761 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     5821 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     5881 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     5941 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     6001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     6061 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     6121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     6181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     6241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     6301 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     6361 ctcacatggc tcgacagatc t 
//
