LOCUS       pCi-N-myc-DEST          5737 bp    DNA     circular     29-JAN-2016
DEFINITION  LMBP 9844.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11084431|
COMMENT     VNTNAME|pCi-N-myc-DEST|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "attR1" 0 0 1 1137 1261 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1265 1321 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 1370 2029 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT        #6=(CFSignal (CObList) "attR2" 0 0 0 2696 2820 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 0 5446 5724 0 (CStringList) 
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COMMENT                                   "@N" 1 15.232 5.22699 0 -10 4.88388 1.16519 
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FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1137..1261)
                     /vntifkey="0"
                     /label=attR1
     promoter        1265..1321
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             1370..2029
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2350..2655
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2696..2820
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(2896..2992)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      2896..2992
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3151..3606
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4045..4905
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      5446..5724
                     /vntifkey="33"
                     /label=pMB1\ori
     misc_marker     1104..1133
                     /vntifkey="22"
                     /label=Myc\epitope
BASE COUNT     1502 a      1314 c      1369 g      1551 t         1 others
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc 
     1081 tctagagtcg acccatggca tcaatgcaga agctgatctc agaggaggac ctgatcacaa 
     1141 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 
     1201 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 
     1261 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 
     1321 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa 
     1381 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 
     1441 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 
     1501 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 
     1561 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 
     1621 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 
     1681 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 
     1741 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 
     1801 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 
     1861 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 
     1921 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat 
     1981 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc 
     2041 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctcgcgaac cggtgtatac 
     2101 ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc 
     2161 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa 
     2221 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag 
     2281 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg 
     2341 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt 
     2401 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc 
     2461 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg 
     2521 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg 
     2581 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg 
     2641 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag 
     2701 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt 
     2761 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt 
     2821 gatgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca 
     2881 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 
     2941 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt 
     3001 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt 
     3061 aaaatcgata aggatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 
     3121 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg 
     3181 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     3241 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     3301 atcgggggct ccctttaggg ttccgattta gagctttacg gcacctcgac cgcaaaaaac 
     3361 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     3421 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     3481 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     3541 taaaaaatga gctgatttaa caaatattta acgcgaattt taacaaaata ttaacgttta 
     3601 caatttcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 
     3661 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 
     3721 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 
     3781 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 
     3841 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 
     3901 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     3961 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     4021 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     4081 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     4141 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     4201 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     4261 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 
     4321 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     4381 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     4441 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     4501 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
     4561 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 
     4621 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
     4681 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
     4741 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
     4801 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
     4861 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
     4921 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     4981 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     5041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     5101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     5161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     5221 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     5281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     5341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     5401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     5461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     5521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     5581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     5641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     5701 ttttgctggc cttttgctca catggctcga cagatct 
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