LOCUS       pCi-N-TagRFP-DEST         6375 bp    DNA     circular     29-JAN-2016
DEFINITION  LMBP 9845.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11084501|
COMMENT     VNTNAME|pCi-N-TagRFP-DEST|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCi-N-TagRFP-DEST" 0 6375 
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COMMENT        (CObList) -1 "") 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 1 5869 5868 1 6375 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #1=(CFSignal (CObList) "T7 promoter" 30 0 0 1034 1050 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "attR1" 0 0 1 1775 1899 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1903 1959 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 2008 2667 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "ccdB" 4 0 0 2988 3293 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "attR2" 0 0 0 3334 3458 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "SV40 polyA early" 43 0 1 3534 3630 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 0 3534 3630 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 0 6084 6362 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "tagRFP" 4 0 0 1064 1756 0 (CStringList) 
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COMMENT                                   "chloramphenicol resistance" "@N" 1 16.4962 
COMMENT                                   -8.71165 0 -10 11.0073 1.16519 #131#) 
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COMMENT                                   (CWideLine 
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COMMENT                                   3.48466 0 -10 8.03237 1.16519 #135#) 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.5637 6.13837 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "hCMV-IE promoter and enhancer" "@N" 1 13.469 
COMMENT                                   12.1963 0 -10 13.065 1.16519 #140#)) 
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COMMENT                      #142=(CGroupWidget 
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COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 5.16942 5.25668) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 14.6929 6.96932 0 -10 4.88388 1.16519 
COMMENT                                   #143#) 
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COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
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COMMENT                                   "lacUV5 promoter" "@N" 1 16.4581 -5.22699 0 
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COMMENT                                   "SV40 polyA early" "@N" 1 -10.0976 -12.1963 0 
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FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1775..1899)
                     /vntifkey="0"
                     /label=attR1
     promoter        1903..1959
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             2008..2667
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2988..3293
                     /vntifkey="4"
                     /label=ccdB
     attenuator      3334..3458
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(3534..3630)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3534..3630
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3789..4244
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4683..5543
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6084..6362
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1064..1756
                     /vntifkey="4"
                     /label=tagRFP
BASE COUNT     1675 a      1514 c      1541 g      1645 t 
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagctag accatggaag agctgattaa 
     1081 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg 
     1141 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt 
     1201 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag 
     1261 cagaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccctga 
     1321 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca 
     1381 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt 
     1441 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca acaccgagat 
     1501 gctgtacccc gctgacggcg gcctggaagg cagaaccgac atggccctga agctcgtggg 
     1561 cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac ccgctaagaa 
     1621 cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca aggaggccga 
     1681 caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag 
     1741 caaactgggg cacaaactct cgagtcgacc catcacaagt ttgtacaaaa aagctgaacg 
     1801 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 
     1861 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 
     1921 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg 
     1981 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 
     2041 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 
     2101 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 
     2161 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 
     2221 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 
     2281 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 
     2341 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 
     2401 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 
     2461 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 
     2521 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 
     2581 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 
     2641 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa 
     2701 cagtatgcgt atttgcgcgc tcgcgaaccg gtgtataccc gaagtatgtc aaaaagaggt 
     2761 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg 
     2821 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg 
     2881 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt 
     2941 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg 
     3001 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta 
     3061 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata 
     3121 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga 
     3181 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc 
     3241 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag 
     3301 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta 
     3361 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca 
     3421 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga tgggcggccg cttcgagcag 
     3481 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 
     3541 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 
     3601 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg 
     3661 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct 
     3721 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 
     3781 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 
     3841 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 
     3901 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 
     3961 ccgatttaga gctttacggc acctcgaccg caaaaaactt gatttgggtg atggttcacg 
     4021 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 
     4081 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     4141 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     4201 aatatttaac gcgaatttta acaaaatatt aacgtttaca atttcgcctg atgcggtatt 
     4261 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct 
     4321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 
     4381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 
     4441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 
     4501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 
     4561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     4621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     4681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     4741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     4801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     4861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     4921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     4981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     5041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     5101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     5161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     5221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     5281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     5341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     5401 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     5461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     5521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     5581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
     5641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     5701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     5761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     5821 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 
     5881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     5941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     6001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     6061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     6121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     6181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     6241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     6301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     6361 tggctcgaca gatct 
//
