LOCUS       pViridis                5029 bp    DNA     circular     14-JUL-2016
DEFINITION  LMBP 9846.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9846
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-JUL-2016
            
            
COMMENT     VNTDATE|-11274554|
COMMENT     VNTNAME|pViridis|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pViridis" 0 5029 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "ApaI" "BamHI" "BstXI" "EcoRI" "EcoRV" "HindIII" "KpnI" 
COMMENT         "NotI" "PstI" "SacI" "SacII" "SalI" "SpeI" "XbaI" "XhoI" "XmaI" "XmnI" 
COMMENT         "PvuI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5029 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2630 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2686 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2718 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2668 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2664 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2656 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2624 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2705 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2678 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2726 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 2717 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2641 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2692 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2698 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2635 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2680 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 802 0 2136 0 3798 0 
COMMENT             0 "") 
COMMENT        #17=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 2467 0 4281 0 0 "")) 
COMMENT       (CObList 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(958..1983)") 
COMMENT        #19=(CFSignal (CObList) "T7 promoter" 30 0 0 2592 2608 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2590..2606") 
COMMENT        #20=(CFSignal (CObList) "pSa ori" 33 0 0 5000 524 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5000..524") 
COMMENT        #21=(CFSignal (CObList) "CaMV 35S promoter" 29 0 1 2003 2396 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2000..2393)") 
COMMENT        #22=(CFSignal (CObList) "CaMV 35S terminator" 43 0 1 587 738 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(587..738)") 
COMMENT        #23=(CFSignal (CObList) "pMB1 ori" 33 0 1 2996 3463 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2995..3463)") 
COMMENT        #24=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 1 3895 4710 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3895..4710)") 
COMMENT        #25=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 2783 2820 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2781..2818)") 
COMMENT        #26=(CFSignal (CObList) "lac promoter" 30 0 1 2821 2904 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2819..2903)") 
COMMENT        #27=(CFSignal (CObList) "T3 promoter" 30 0 1 2741 2757 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2739..2755)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "LMBP 9846" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "Last modified: 14-JUL-2016" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pViridis'" 1) 
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COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 06/09/16 12:29" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/14/16 03:17" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5029 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 7-JUL-2016" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 9846") 
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COMMENT                                        "Start: 958   End: 1983 (Complementary)" 
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COMMENT                                   #58=(CLinePar 
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COMMENT                                        "nptI gene confers resistance to kanamycin and neomycin" 
COMMENT                                        1) 
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COMMENT                                 "CaMV 35S promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "   complement(2819..2903)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                 "5' UTR of lacZ, incl. RBS"))) "RBS (1 total)") 
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COMMENT                                        "   complement(2995..3463)" 1))) 
COMMENT                                 "pMB1 ori") 
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COMMENT                                        "Start: 5000  End: 524 " 1) 
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COMMENT                                   #92=(CLinePar 
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COMMENT                                        "   5000..524" 1))) "pSa ori"))) 
COMMENT                          "Replication Origin (2 total)") 
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COMMENT                                        "Start: 587   End: 738  (Complementary)" 
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COMMENT                                        "   complement(587..738)" 1))) 
COMMENT                                 "CaMV 35S terminator"))) "Terminator (1 total)"))) 
COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     CDS             complement(958..1983)
                     /vntifkey="4"
                     /label=hygromycin\resistance
     promoter        2592..2608
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      5000..524
                     /vntifkey="33"
                     /label=pSa\ori
     promoter        complement(2003..2396)
                     /vntifkey="29"
                     /label=CaMV\35S\promoter
     terminator      complement(587..738)
                     /vntifkey="43"
                     /label=CaMV\35S\terminator
     rep_origin      complement(2996..3463)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3895..4710)
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
                     /note="nptI gene confers resistance to kanamycin and neomycin"
     RBS             complement(2783..2820)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(2821..2904)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        complement(2741..2757)
                     /vntifkey="30"
                     /label=T3\promoter
BASE COUNT     1252 a      1285 c      1240 g      1252 t 
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc 
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg 
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg 
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc 
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat 
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac 
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc 
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa 
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc 
      541 ttggcaggat atattgtggt gtaacgttat cgatctggat tttagtactg gattttggtt 
      601 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt 
      661 ttcttatatg ctcaacacat gagcgaaacc ctataagaac cctaatttcc cttatcggga 
      721 aactactcac acattattta tggagaaaat agagagagat agatttgtag agagagactg 
      781 gtgatttcag cgtaccgaat taattctccc gcgaccagcc gagcgagctt agcgaactgt 
      841 ggacgagaac tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg 
      901 cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta 
      961 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac 
     1021 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc 
     1081 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt 
     1141 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag 
     1201 gcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat 
     1261 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa 
     1321 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt 
     1381 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat 
     1441 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca 
     1501 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc 
     1561 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat 
     1621 agcatccata gcctccgcga ccggctgaag aacagcgggc agttcggttt caggcaggtc 
     1681 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaactc 
     1741 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata 
     1801 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc 
     1861 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac 
     1921 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt 
     1981 catagggggg atcagcttgg gctgtcctct ccaaatgaaa tgaacttcct tatatagagg 
     2041 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatgtcac 
     2101 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg 
     2161 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaatg atagcctttc 
     2221 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttctactgtc ctttcgatga 
     2281 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgaaattacc ctttgttgaa 
     2341 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gacatttttg gagtaggggt 
     2401 acgataacat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 
     2461 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 
     2521 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 
     2581 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg 
     2641 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta 
     2701 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg 
     2761 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     2821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     2881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     2941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     3001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     3061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     3121 atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga 
     3181 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     3241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     3301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3421 ggaagcgtgg cgctttctca tagctcacgc tgtagtatct cagttcggtg taggtcgttc 
     3481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     3541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     3601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     3661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     3721 ttaccttcgg aagaagagtt ggtagctctt gatggctcaa acatgaagaa atgaaatgac 
     3781 tgagtcagcc gagaagaatt tccccgctta ttcgcacctt ccctagatcc ttttaaatta 
     3841 aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa 
     3901 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata 
     3961 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat 
     4021 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa 
     4081 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc 
     4141 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt 
     4201 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg 
     4261 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa 
     4321 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc 
     4381 taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg 
     4441 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct 
     4501 gaccatctca tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc 
     4561 tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc 
     4621 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga 
     4681 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc 
     4741 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt 
     4801 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat 
     4861 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa 
     4921 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga 
     4981 tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggct 
//
