ID pViridis standard; circular DNA; ; 5029 BP. DE LMBP 9846. CC Accession number: LMBP 9846 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 14-JUL-2016 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(958..1983) FT /vntifkey="4" FT /label=hygromycin\resistance FT promoter 2592..2608 FT /vntifkey="30" FT /label=T7\promoter FT rep_origin 5000..524 FT /vntifkey="33" FT /label=pSa\ori FT promoter complement(2003..2396) FT /vntifkey="29" FT /label=CaMV\35S\promoter FT terminator complement(587..738) FT /vntifkey="43" FT /label=CaMV\35S\terminator FT rep_origin complement(2996..3463) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(3895..4710) FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT /note="nptI gene confers resistance to kanamycin and neomycin" FT RBS complement(2783..2820) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT promoter complement(2821..2904) FT /vntifkey="30" FT /label=lac\promoter FT promoter complement(2741..2757) FT /vntifkey="30" FT /label=T3\promoter SQ Sequence 5029 BP; 1252 A; 1285 C; 1240 G; 1252 t; tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc 60 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg 120 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg 180 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc 240 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat 300 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac 360 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc 420 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa 480 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc 540 ttggcaggat atattgtggt gtaacgttat cgatctggat tttagtactg gattttggtt 600 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt 660 ttcttatatg ctcaacacat gagcgaaacc ctataagaac cctaatttcc cttatcggga 720 aactactcac acattattta tggagaaaat agagagagat agatttgtag agagagactg 780 gtgatttcag cgtaccgaat taattctccc gcgaccagcc gagcgagctt agcgaactgt 840 ggacgagaac tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg 900 cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta 960 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac 1020 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc 1080 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt 1140 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag 1200 gcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat 1260 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa 1320 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt 1380 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat 1440 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca 1500 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc 1560 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat 1620 agcatccata gcctccgcga ccggctgaag aacagcgggc agttcggttt caggcaggtc 1680 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaactc 1740 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata 1800 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc 1860 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac 1920 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt 1980 catagggggg atcagcttgg gctgtcctct ccaaatgaaa tgaacttcct tatatagagg 2040 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatgtcac 2100 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg 2160 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaatg atagcctttc 2220 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttctactgtc ctttcgatga 2280 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgaaattacc ctttgttgaa 2340 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gacatttttg gagtaggggt 2400 acgataacat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 2460 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 2520 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 2580 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg 2640 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta 2700 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg 2760 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 2820 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 2880 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 2940 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 3000 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 3060 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 3120 atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga 3180 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 3240 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 3300 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 3360 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3420 ggaagcgtgg cgctttctca tagctcacgc tgtagtatct cagttcggtg taggtcgttc 3480 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 3540 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 3600 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 3660 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 3720 ttaccttcgg aagaagagtt ggtagctctt gatggctcaa acatgaagaa atgaaatgac 3780 tgagtcagcc gagaagaatt tccccgctta ttcgcacctt ccctagatcc ttttaaatta 3840 aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa 3900 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata 3960 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat 4020 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa 4080 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc 4140 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt 4200 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg 4260 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa 4320 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc 4380 taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg 4440 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct 4500 gaccatctca tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc 4560 tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc 4620 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga 4680 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc 4740 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt 4800 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat 4860 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa 4920 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga 4980 tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggct 5029 //