LOCUS       pENTR3C-NvPCASPt1A        4252 bp    DNA     circular SYN  4-MAY-2016
DEFINITION  LMBP 9875.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11223006|
COMMENT     VNTDBDATE|-11223008|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-NvPCASPt1A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR3C-NvPCASPt1A" 0 4252 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "EcoRI" "XhoI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" 
COMMENT         "AciI" "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" 
COMMENT         "AloI" "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" 
COMMENT         "BbeI" "Bbr7I" "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" 
COMMENT         "BciVI" "BclI" "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" 
COMMENT         "BmrI" "BmtI" "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" 
COMMENT         "BsaI" "BsaJI" "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" 
COMMENT         "BsiEI" "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" 
COMMENT         "Bsp1286I" "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" 
COMMENT         "BsrFI" "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" 
COMMENT         "BstF5I" "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" 
COMMENT         "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" 
COMMENT         "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" 
COMMENT         "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XmaI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4252 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1357 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2468 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 67 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 2192 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AceIII" "cagctc" 14 18 2 1706 0 2605 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AciI" "ccgc" 2 4 26 43 0 191 0 200 1 238 1 251 
COMMENT            0 1054 1 1125 0 1553 1 1593 1 1832 1 1952 0 1977 1 2098 0 2149 1 
COMMENT            2222 0 2224 1 2308 1 2734 0 3655 0 3664 1 3799 0 3909 1 4030 1 4049 
COMMENT            1 4176 1 4204 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "AclI" "aacgtt" 3 0 1 210 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 2 1018 1 3705 1 0 "") 
COMMENT        #10=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 344 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AflIII" "acrygt" 2 0 1 4247 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AluI" "agct" 3 0 16 349 0 571 0 670 0 676 0 
COMMENT             1177 0 1238 0 1696 0 1911 0 2110 0 2487 0 2589 0 2595 0 3690 0 3947 
COMMENT             0 3993 0 4083 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AlwI" "ggatc" 10 11 9 61 0 1179 0 1931 1 1944 
COMMENT             0 2991 1 3386 0 3601 1 3603 0 3689 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 2 2300 0 3838 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 1 1134 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 357 0 1543 0 3555 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 3933 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "ApoI" "raatty" 2 0 6 1010 0 1357 0 2241 0 
COMMENT             2319 0 2742 0 2926 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AseI" "attaat" 3 0 1 3328 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 3129 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 4 57 0 350 0 1398 0 2468 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 790 0 1309 0 1927 0 2047 
COMMENT             0 0 "") #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1936 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BanII" "grgcyc" 6 0 8 357 0 618 0 678 0 1543 
COMMENT             0 1952 0 2112 0 2784 0 3555 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 140 0 1823 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 135 0 1818 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BbvI" "gcagc" 14 18 9 1063 0 1225 1 1986 0 
COMMENT             2576 1 2604 0 3619 1 3825 1 3828 1 3918 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BccI" "ccatc" 10 11 10 293 1 296 0 1022 1 
COMMENT             1289 1 1402 1 1657 1 2590 0 2895 1 2953 0 3394 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BceAI" "acggc" 18 20 7 239 0 1095 1 1474 0 
COMMENT             1625 1 1774 0 3448 0 3747 1 0 "") 
COMMENT        #41=(CRSite (CStringList) "BcefI" "acggc" 18 19 7 239 0 1096 1 1474 0 
COMMENT             1626 1 1774 0 3448 0 3748 1 0 "") 
COMMENT        #42=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 1 38 1 0 "") 
COMMENT        #43=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 790 0 1700 1 4049 0 0 
COMMENT             "") #44=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BfaI" "ctag" 2 0 3 46 0 2478 0 3754 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 4 1585 0 1733 0 2977 0 
COMMENT             3243 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BglII" "agatct" 2 0 2 1214 0 2124 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 4 357 0 1543 0 3555 0 
COMMENT             3937 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 1801 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BmrI" "actggg" 12 11 5 142 0 940 1 1546 0 
COMMENT             1882 1 2288 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 49 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 1315 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 692 0 2009 0 2126 0 0 
COMMENT             "") #56=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 1 3146 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 2227 0 3623 0 3864 1 
COMMENT             4162 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 3 2017 0 2252 0 2384 0 0 
COMMENT             "") #59=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 64 0 247 0 899 0 1524 0 
COMMENT             1689 0 0 "") #61=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 9 793 0 1254 0 1441 0 
COMMENT             1480 0 2029 0 2222 0 3000 0 3402 0 4087 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 5 1168 0 2049 0 3264 0 
COMMENT             3894 0 4041 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BscAI" "gcatc" 10 12 17 108 0 276 0 818 0 
COMMENT             1206 0 1318 1 1575 1 1723 1 1782 0 2027 1 2381 1 2748 1 2832 1 2967 
COMMENT             1 3054 1 3174 1 3485 1 4153 1 0 "") 
COMMENT        #66=(CRSite (CStringList) "BseMII" "ctcag" 16 14 12 134 0 167 1 857 1 
COMMENT             1281 1 1363 0 1936 0 1937 1 2155 0 2268 1 3160 0 3555 1 3964 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BseRI" "gaggag" 17 15 4 908 0 1430 1 1973 0 
COMMENT             2260 1 0 "") 
COMMENT        #68=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 1692 0 2483 0 3943 0 0 
COMMENT             "") 
COMMENT        #69=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 2 753 0 1805 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 2 3129 0 3913 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 6 678 0 2028 0 2061 0 
COMMENT             2112 0 2251 0 3937 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 18 234 0 774 0 1093 0 
COMMENT             1208 0 1295 0 1549 0 1722 0 1831 0 1957 0 2044 0 2104 0 3043 0 3375 
COMMENT             0 3392 0 3769 0 4048 0 4214 0 4232 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 8 648 0 890 1 1925 0 2099 
COMMENT             0 2111 1 2144 0 2612 0 3145 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 890 1 3145 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsmFI" "gggac" 16 20 4 75 0 1547 0 1940 0 
COMMENT             2282 1 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 3 580 1 3013 1 3090 1 0 "") 
COMMENT        #78=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 12 357 0 618 0 678 0 
COMMENT             1543 0 1952 0 2028 0 2061 0 2112 0 2251 0 2784 0 3555 0 3937 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 2 2286 1 3553 1 
COMMENT             0 "") 
COMMENT        #80=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 12 133 0 168 1 858 1 
COMMENT             1282 1 1362 0 1935 0 1938 1 2154 0 2269 1 3159 0 3556 1 3965 1 0 "") 
COMMENT        #81=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 880 0 2653 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 2203 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 2 200 1 2308 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 5 422 0 1138 0 2445 1 
COMMENT             2519 1 2627 1 0 "") 
COMMENT        #86=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 2 1475 0 3083 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 3 441 0 1561 0 2494 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "BsrI" "actgg" 7 5 14 137 0 650 1 946 1 1015 1 
COMMENT             1341 0 1541 0 1708 0 1888 1 2072 0 2294 1 2920 0 3720 0 3832 1 3845 
COMMENT             1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 1 1589 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BssSI" "cacgag" 2 6 1 4074 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 1 1133 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 2 1265 0 2001 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 12 288 1 304 0 917 1 1033 
COMMENT             0 1198 1 1300 0 1867 0 2039 0 2393 0 2582 1 2760 0 3386 1 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstKTI" "gatc" 4 0 17 56 0 1174 0 1217 0 1263 
COMMENT             0 1939 0 2022 0 2127 0 2265 0 2364 0 2418 0 2999 0 3129 0 3381 0 
COMMENT             3590 0 3598 0 3609 0 3684 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 8 97 0 265 0 794 0 3019 0 
COMMENT             3376 0 4088 0 4101 0 4222 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "BstUI" "cgcg" 3 0 11 1591 0 1593 0 1832 0 
COMMENT             2100 0 2135 0 2224 0 2736 0 2786 0 3131 0 3623 0 4204 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 487 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 7 53 0 1214 0 1936 0 2124 
COMMENT             0 3378 0 3595 0 3606 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 2 1290 0 1349 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 3 1480 0 2029 0 2222 0 0 
COMMENT              "") 
COMMENT        #103=(CRSite (CStringList) "BthCI" "gcngc" 5 0 15 1054 0 1057 0 1241 0 
COMMENT              1596 0 1835 0 1977 0 2592 0 2595 0 2736 0 3635 0 3841 0 3844 0 
COMMENT              3909 0 4052 0 4207 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 2 2990 1 3077 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 19 47 0 240 0 251 0 454 
COMMENT              0 1129 0 1161 0 1463 0 1477 0 1587 0 1591 0 1952 0 2102 0 2289 0 
COMMENT              2784 0 3068 0 3214 0 3637 0 4197 0 4234 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "CdiI" "catcg" 5 5 3 1729 1 2838 1 3498 1 0 
COMMENT              "") 
COMMENT        #107=(CRSite (CStringList) "ChaI" "gatc" 5 0 17 57 0 1175 0 1218 0 1264 
COMMENT              0 1940 0 2023 0 2128 0 2266 0 2365 0 2419 0 3000 0 3130 0 3382 0 
COMMENT              3591 0 3599 0 3610 0 3685 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "CjeI" "ccannnnnngt" 27 21 7 2051 1 2319 0 
COMMENT              3586 0 3650 1 3686 0 3764 0 4205 1 0 "") 
COMMENT        #109=(CRSite (CStringList) "CjePI" "ccannnnnnntc" 27 21 8 715 0 1392 1 
COMMENT              1520 1 2319 0 2962 1 3214 1 3586 0 3699 1 0 "") 
COMMENT        #110=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "Csp6I" "gtac" 2 0 11 442 0 562 0 653 0 1562 
COMMENT              0 1817 0 1867 0 1891 0 2219 0 2253 0 2495 0 2963 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "CviAII" "catg" 2 0 18 50 0 481 0 487 0 523 0 
COMMENT              881 0 1043 0 1107 0 1363 0 1481 0 1510 0 1586 0 2256 0 2654 0 2751 
COMMENT              0 2863 0 2978 0 3283 0 4248 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "CviJI" "rgcy" 3 0 71 119 0 137 0 226 0 287 0 
COMMENT              303 0 349 0 355 0 456 0 498 0 571 0 590 0 616 0 663 0 670 0 676 0 
COMMENT              689 0 696 0 831 0 943 0 1002 0 1032 0 1057 0 1163 0 1177 0 1238 0 
COMMENT              1299 0 1340 0 1418 0 1461 0 1479 0 1485 0 1500 0 1541 0 1640 0 
COMMENT              1655 0 1696 0 1911 0 1950 0 1987 0 2071 0 2110 0 2287 0 2398 0 
COMMENT              2423 0 2487 0 2589 0 2595 0 2601 0 2708 0 2733 0 2782 0 2835 0 
COMMENT              2919 0 3212 0 3216 0 3435 0 3553 0 3690 0 3719 0 3762 0 3773 0 
COMMENT              3838 0 3917 0 3942 0 3947 0 3993 0 4083 0 4181 0 4207 0 4225 0 
COMMENT              4236 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "DdeI" "ctnag" 2 0 14 75 0 120 0 176 0 276 0 
COMMENT              866 0 1290 0 1349 0 1922 0 1946 0 2141 0 2277 0 3146 0 3564 0 3973 
COMMENT              0 0 "") 
COMMENT        #115=(CRSite (CStringList) "DpnI" "gatc" 3 0 17 55 0 1173 0 1216 0 1262 
COMMENT              0 1938 0 2021 0 2126 0 2264 0 2363 0 2417 0 2998 0 3128 0 3380 0 
COMMENT              3589 0 3597 0 3608 0 3683 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "DraI" "tttaaa" 4 0 2 462 0 1372 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 2 1553 0 4145 0 0 
COMMENT              "") #119=(CRSite (CStringList) "EaeI" "yggccr" 2 0 1 1477 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "EagI" "cggccg" 2 0 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "EarI" "ctcttc" 8 11 6 330 0 651 0 885 1 1628 
COMMENT              1 2388 1 2942 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "EciI" "ggcgga" 18 16 3 180 1 4045 0 4191 0 0 
COMMENT              "") 
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COMMENT                             "CTGGAGNNNNNNNNNNNNNNNNN" "GACCTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #400=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305632 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #401=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #56# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #402=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #56# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 3146 (on complementary strand)" 
COMMENT                                             1))))) "Bpu10I: 1 site") 2 5 
COMMENT                             "CCTNAGC" "GGANTCG") 
COMMENT                       #403=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305664 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BpuEI: 4 sites") 22 20 
COMMENT                             "CTTGAGNNNNNNNNNNNNNNNNN" "GAACTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #404=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305696 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaAI: 3 sites") 3 3 "YACGTR" 
COMMENT                             "RTGCAY") 
COMMENT                       #405=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305728 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaBI: 0 sites") 5 5 "GATNNNNATC" 
COMMENT                             "CTANNNNTAG") 
COMMENT                       #406=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305760 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaHI: 5 sites") 2 4 "GRCGYC" 
COMMENT                             "CYGCRG") 
COMMENT                       #407=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305792 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaI: 0 sites") 7 11 
COMMENT                             "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                       #408=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305824 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaJI: 9 sites") 1 5 "CCNNGG" 
COMMENT                             "GGNNCC") 
COMMENT                       #409=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305856 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaWI: 5 sites") 1 5 "WCCGGW" 
COMMENT                             "WGGCCW") 
COMMENT                       #410=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305888 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaXI: 0 sites") 21 18 
COMMENT                             "ACNNNNNCTCCNNNNNNNNNNN" "TGNNNNNGAGGNNNNNNNNNNN") 
COMMENT                       #411=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305920 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BscAI: 17 sites") 9 11 
COMMENT                             "GCATCNNNNNNN" "CGTAGNNNNNNN") 
COMMENT                       #412=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305952 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseMII: 12 sites") 15 13 
COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
COMMENT                       #413=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56305984 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseRI: 4 sites") 16 14 
COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
COMMENT                       #414=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306016 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseYI: 3 sites") 1 5 "CCCAGC" 
COMMENT                             "GGGTCG") 
COMMENT                       #415=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306048 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #416=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #69# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 753 " 1) 
COMMENT                                       #418=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #69# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 1805 (on complementary strand)" 
COMMENT                                             1))))) "BsgI: 2 sites") 22 20 
COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #419=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306080 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #420=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #70# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #421=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #70# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3129 " 1) 
COMMENT                                       #422=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #70# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3913 " 1))))) 
COMMENT                              "BsiEI: 2 sites") 4 2 "CGRYCG" "GCYRGC") 
COMMENT                       #423=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306112 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiHKAI: 6 sites") 5 1 "GWGCWC" 
COMMENT                             "CWCGWG") 
COMMENT                       #424=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306144 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiWI: 0 sites") 1 5 "CGTACG" 
COMMENT                             "GCATGC") 
COMMENT                       #425=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306176 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BslI: 18 sites") 7 4 
COMMENT                             "CCNNNNNNNGG" "GGNNNNNNNCC") 
COMMENT                       #426=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306208 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmAI: 8 sites") 6 10 
COMMENT                             "GTCTCNNNNNN" "CAGAGNNNNNN") 
COMMENT                       #427=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306240 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #428=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #75# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #429=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #75# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 890 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #430=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #75# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3145 " 1))))) 
COMMENT                              "BsmBI: 2 sites") 7 11 "CGTCTCNNNNNN" 
COMMENT                             "GCAGAGNNNNNN") 
COMMENT                       #431=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306272 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmFI: 4 sites") 15 19 
COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
COMMENT                       #432=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306304 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmI: 3 sites") 7 5 "GAATGCNN" 
COMMENT                             "CTTACGNN") 
COMMENT                       #433=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 55503264 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsp1286I: 12 sites") 5 1 "GDGCHC" 
COMMENT                             "CHCGDG") 
COMMENT                       #434=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 55503304 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #435=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #79# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #436=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 2286 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #437=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 3553 (on complementary strand)" 
COMMENT                                             1))))) "Bsp24I: 2 sites") 24 19 
COMMENT                             "GACNNNNNNTGGNNNNNNNNNNNNN" 
COMMENT                             "CTGNNNNNNACCNNNNNNNNNNNNN") 
COMMENT                       #438=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306336 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspCNI: 12 sites") 14 12 
COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
COMMENT                       #439=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306368 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspEI: 0 sites") 1 5 "TCCGGA" 
COMMENT                             "AGGCCT") 
COMMENT                       #440=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306400 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #441=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #82# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #442=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #82# 1) 1 2 2 0 
COMMENT                                              191) " N1: 880 " 1) 
COMMENT                                       #443=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #82# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2653 " 1))))) 
COMMENT                              "BspHI: 2 sites") 1 5 "TCATGA" "AGTACT") 
COMMENT                       #444=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306432 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #445=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #83# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #446=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #83# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 2203 (on complementary strand)" 
COMMENT                                             1))))) "BspMI: 1 site") 10 14 
COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
COMMENT                       #447=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306464 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #448=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #84# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #449=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #84# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 200 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #450=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #84# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 2308 (on complementary strand)" 
COMMENT                                             1))))) "BsrBI: 2 sites") 3 3 
COMMENT                             "CCGCTC" "GGCGAG") 
COMMENT                       #451=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306496 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrDI: 5 sites") 8 6 "GCAATGNNN" 
COMMENT                             "CGTTACNNN") 
COMMENT                       #452=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #453=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #86# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #454=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #86# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1475 " 1) 
COMMENT                                       #455=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #86# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3083 " 1))))) 
COMMENT                              "BsrFI: 2 sites") 1 5 "RCCGGY" "YGGCCR") 
COMMENT                       #456=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306560 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrGI: 3 sites") 1 5 "TGTACA" 
COMMENT                             "ACATGT") 
COMMENT                       #457=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306592 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrI: 14 sites") 6 4 "ACTGGNN" 
COMMENT                             "TGACCNN") 
COMMENT                       #458=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306624 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #459=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #89# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #460=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #89# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1589 " 1))))) 
COMMENT                              "BssHII: 1 site") 1 5 "GCGCGC" "CGCGCG") 
COMMENT                       #461=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306656 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #462=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #90# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #463=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #90# 1) 1 2 2 0 
COMMENT                                              191) " N1: 4074 " 1))))) 
COMMENT                              "BssSI: 1 site") 1 5 "CACGAG" "GTGCTC") 
COMMENT                       #464=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306688 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #465=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #91# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #466=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #91# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1133 " 1))))) 
COMMENT                              "BstAPI: 1 site") 7 4 "GCANNNNNTGC" "CGTNNNNNACG") 
COMMENT                       #467=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306720 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstBI: 0 sites") 2 4 "TTCGAA" 
COMMENT                             "AAGCTT") 
COMMENT                       #468=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306752 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #469=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #93# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #470=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #93# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1265 " 1) 
COMMENT                                       #471=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #93# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2001 " 1))))) 
COMMENT                              "BstEII: 2 sites") 1 6 "GGTNACC" "CCANTGG") 
COMMENT                       #472=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306784 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstF5I: 12 sites") 7 5 "GGATGNNN" 
COMMENT                             "CCTACNNN") 
COMMENT                       #473=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306816 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstKTI: 17 sites") 3 1 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #474=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306848 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstNI: 8 sites") 2 3 "CCWGG" 
COMMENT                             "GGWCC") 
COMMENT                       #475=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306880 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstUI: 11 sites") 2 2 "CGCG" 
COMMENT                             "GCGC") 
COMMENT                       #476=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306912 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #477=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #98# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #478=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #98# 1) 1 2 2 0 
COMMENT                                              191) " N1: 487 " 1))))) 
COMMENT                              "BstXI: 1 site") 8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                       #479=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306944 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstYI: 7 sites") 1 5 "RGATCY" 
COMMENT                             "YCTAGR") 
COMMENT                       #480=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56306976 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstZ17I: 0 sites") 3 3 "GTATAC" 
COMMENT                             "CATATG") 
COMMENT                       #481=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307008 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #482=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #101# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #483=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #101# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1290 " 1) 
COMMENT                                       #484=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #101# 2) 1 2 2 0 
COMMENT                                              191) " N2: 1349 " 1))))) 
COMMENT                              "Bsu36I: 2 sites") 2 5 "CCTNAGG" "GGANTCC") 
COMMENT                       #485=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307040 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtgI: 3 sites") 1 5 "CCRYGG" 
COMMENT                             "GGYRCC") 
COMMENT                       #486=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307072 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BthCI: 15 sites") 4 1 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #487=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307104 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #488=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #104# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #489=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #104# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 2990 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #490=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #104# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3077 " 1))))) 
COMMENT                              "BtsI: 2 sites") 8 6 "GCAGTGNNN" "CGTCACNNN") 
COMMENT                       #491=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307136 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Cac8I: 19 sites") 3 3 "GCNNGC" 
COMMENT                             "CGNNCG") 
COMMENT                       #492=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307168 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CdiI: 3 sites") 4 4 "CATCG" 
COMMENT                             "GTAGC") 
COMMENT                       #493=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307200 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ChaI: 17 sites") 5 1 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #494=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjeI: 7 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNCANNNNNNNNNNNNNNNN") 
COMMENT                       #495=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307264 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjePI: 8 sites") 26 20 
COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNNAGNNNNNNNNNNNNNNN") 
COMMENT                       #496=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307296 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                             "TAGCTA") 
COMMENT                       #497=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307328 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Csp6I: 11 sites") 1 3 "GTAC" 
COMMENT                             "CATG") 
COMMENT                       #498=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307360 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviAII: 18 sites") 1 3 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #499=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307392 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviJI: 71 sites") 2 2 "RGCY" 
COMMENT                             "YCGR") 
COMMENT                       #500=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307424 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DdeI: 14 sites") 1 4 "CTNAG" 
COMMENT                             "GANTC") 
COMMENT                       #501=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307456 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DpnI: 17 sites") 2 2 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #502=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307488 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #503=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #116# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #504=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #116# 1) 1 2 2 0 
COMMENT                                              191) " N1: 462 " 1) 
COMMENT                                       #505=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #116# 2) 1 2 2 0 
COMMENT                                              191) " N2: 1372 " 1))))) 
COMMENT                              "DraI: 2 sites") 3 3 "TTTAAA" "AAATTT") 
COMMENT                       #506=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307520 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DraIII: 0 sites") 6 3 "CACNNNGTG" 
COMMENT                             "GTGNNNCAC") 
COMMENT                       #507=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56307552 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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FEATURES             Location/Qualifiers
     misc_recomb     358..457
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     misc_recomb     complement(2481..2580)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     CDS             2703..3512
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      3576..4249
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     misc_feature    458..459
                     /vntifkey="21"
                     /label=NN
     CDS             481..2466
                     /vntifkey="4"
                     /label=NvPCASPt1A
     misc_feature    480..480
                     /vntifkey="21"
                     /label=+C
     CDS             460..480
                     /vntifkey="4"
                     /label=translated\sequence\if\N-term\fusion
     misc_feature    2464..2466
                     /vntifkey="21"
                     /label=Stop
BASE COUNT     1131 a      1000 c      1052 g      1069 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcc 
      481 atgcgcatgg gtggtagccc taccgagtgc cttcttcaag acatgagaac aaaatgcagg 
      541 acagtcaagc agttggtatc gtacttggag ctaatggagc attcagaggc tttggaatta 
      601 ctgaaacctg atgagccccc tgtcatcaca gaacaaccaa tgtctcttcc agtacaagaa 
      661 ggcttgaagc tggagctcac ttgtagagcc acaggctttc ctccccctca atatcaatgg 
      721 gtgaaaaatg gtgcagaatt accttatggg tgtgactcca ccctgaccat agacagggta 
      781 tcccgacagg accagggcaa atacttctgc atcataagca acaggatagg ctctgttaag 
      841 acttatgagg ttgaagtcaa tgttctgagg gtgcaacgtc atgaacaaga agaggagacg 
      901 cccttacctg aaagttcatc cctcccttac atcactcaac agccccagtc tgctctcatt 
      961 cctattggac acagatatca cctcacttgt ggaaccaaag gccctgtcaa attccagtgg 
     1021 ttcaaggatg gcttcagatt acatgagggg cagcggccta atctccattt tcccacattc 
     1081 aacttccaag atgagggcaa ctatacatgc cgtgtgtcca acaccgcagg caatgccctg 
     1141 tctgcaactg ctaccctgca ggccttaccg gatcagcttg acataaatgc tggacagcat 
     1201 ccagatgtag gtagatctat ggagttatcc cagacagctg cagacaaagt tgccttggtg 
     1261 atcggtaacc gtgactacat caatctacct gaggatggct ctccattggt ccattcgtgt 
     1321 agtgatgctc aaagactggc ttctgtcctc aggaagaatt ccatgaactt taaagttgtg 
     1381 tcaatggtta acttaacccg agtagagatg gacaaggcta tcaatttatt cctcgacctc 
     1441 ctcggtgaaa atgtttacgg cttgctatac ttcgccggcc atggcttcga ggaacagggc 
     1501 cagaactaca tggttcctat agacgccgag agggactggg cccctgacga ggcggtctgt 
     1561 gtacagaagg tgttcgagat gatgcatgcg cgcggcactc aactgaacat cttccttctg 
     1621 gacatctgca ggaagagagc cgtgacgagt caggcctaca agatggaaac ctactccttt 
     1681 ccatttgacg cccagctcgt cactggatac gccacctgta aagggagcga tgcatacgag 
     1741 cgaaagagtg acagtaacgg catttatatg aagcatctcc tgaataacat cacgttggac 
     1801 gtgaaggtgg aagacgtact gcaccgagtc gcggcagacg ttgaggacga ggtcgctatg 
     1861 gatgagtaca cagagcgtca gcgaccccag tacgatagca agacttcaag ctctttcagt 
     1921 ctcagggacc ctgtggatcc aaaactgagc ccgcaagagg agcgagaaca gcgcagcggt 
     1981 atttggctgg agttgcatag gttaccagaa aagcacgtga tcgagcaccg tggatgccgg 
     2041 gtcgaggtcc ggttcgagca cactaactgg ctttctaacg taatcgtggt ctgtctccgc 
     2101 gtgctggagc tcggcgagac aaagatctgt gacgcgagtc tcagtttgcg gaatctgcca 
     2161 caggactttc aagactgcca ttttcacccg tcgacgacaa acatcttgag gcaggtcgta 
     2221 ccgcgggagt atcaagacga aattcgagca cgtacatgga agatcgactc ctcccctgag 
     2281 aaagagcctg ccccagtccc tgtcgagcgg ataagcgaaa tttacaacat acaaaggtta 
     2341 aagaaaaacc tgaccttaac gatcaagtta aagtatgtgc tacgtggatg cgaagagcca 
     2401 gagagcaaat acgagatcaa ggcctttaac atcaaagagt tcggcattgc tatggcattt 
     2461 tgctaactcg agatatctag acccagcttt cttgtacaaa gttggcatta taagaaagca 
     2521 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 
     2581 ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 
     2641 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg 
     2701 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg 
     2761 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 
     2821 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 
     2881 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 
     2941 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 
     3001 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 
     3061 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 
     3121 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 
     3181 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 
     3241 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 
     3301 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 
     3361 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 
     3421 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 
     3481 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacatt 
     3541 attcagattg ggccccgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     3601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     3661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     3721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 
     3781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     3841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     3901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     3961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     4021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     4081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     4141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     4201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt 
//

