LOCUS       pENTR3C-NvPCASPt1B        4469 bp    DNA     circular SYN  4-MAY-2016
DEFINITION  LMBP 9876.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11222978|
COMMENT     VNTDBDATE|-11222981|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-NvPCASPt1B|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR3C-NvPCASPt1B" 0 4469 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "EcoRI" "XhoI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" 
COMMENT         "AciI" "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" 
COMMENT         "AloI" "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" 
COMMENT         "BbeI" "Bbr7I" "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" 
COMMENT         "BciVI" "BclI" "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" 
COMMENT         "BmrI" "BmtI" "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" 
COMMENT         "BsaI" "BsaJI" "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" 
COMMENT         "BsiEI" "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" 
COMMENT         "Bsp1286I" "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" 
COMMENT         "BsrFI" "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" 
COMMENT         "BstF5I" "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" 
COMMENT         "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" 
COMMENT         "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" 
COMMENT         "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XmaI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4469 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 2026 0 2426 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1488 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 67 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 479 0 1956 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AceIII" "cagctc" 14 18 2 2556 1 2822 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AciI" "ccgc" 2 4 30 43 0 191 0 200 1 238 1 251 
COMMENT            0 666 0 754 1 1408 1 1421 1 1514 0 1760 1 1845 0 2021 0 2105 0 2240 
COMMENT            1 2337 1 2423 1 2451 0 2508 1 2577 0 2635 0 2951 0 3872 0 3881 1 
COMMENT            4016 0 4126 1 4247 1 4266 1 4393 1 4421 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "AclI" "aacgtt" 3 0 1 210 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 3 593 1 1706 0 3922 1 0 
COMMENT            "") #10=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 344 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AflIII" "acrygt" 2 0 4 1440 0 2217 0 2436 0 
COMMENT             4464 0 0 "") #13=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 1 878 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 1 1682 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AluI" "agct" 3 0 18 349 0 501 0 715 0 794 0 
COMMENT             895 0 1085 0 1135 0 1207 0 2086 0 2228 0 2570 0 2704 0 2806 0 2812 
COMMENT             0 3907 0 4164 0 4210 0 4300 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AlwI" "ggatc" 10 11 9 61 0 480 1 493 0 2353 1 
COMMENT             3208 1 3603 0 3818 1 3820 0 3906 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 916 0 2685 0 4055 0 
COMMENT             0 "") #20=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 357 0 3772 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 2189 0 4150 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "ApoI" "raatty" 2 0 6 617 0 2026 0 2154 0 2426 
COMMENT             0 2959 0 3143 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AseI" "attaat" 3 0 1 3545 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 3346 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 57 0 350 0 1488 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 648 0 1333 0 1467 0 2351 
COMMENT             0 2632 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 485 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 1 1510 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 357 0 796 0 2665 0 3001 
COMMENT             0 3772 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 4 140 0 589 1 2378 0 
COMMENT             2402 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BbsI" "gaagac" 9 13 4 135 0 594 1 2373 0 2407 
COMMENT             1 0 "") #37=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BbvI" "gcagc" 14 18 10 1594 0 2303 0 2592 1 
COMMENT             2686 0 2793 1 2821 0 3836 1 4042 1 4045 1 4135 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BccI" "ccatc" 10 11 8 293 1 296 0 1376 1 1687 
COMMENT             1 2807 0 3112 1 3170 0 3611 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BceAI" "acggc" 18 20 5 239 0 1978 0 2249 1 
COMMENT             3665 0 3964 1 0 "") 
COMMENT        #41=(CRSite (CStringList) "BcefI" "acggc" 18 19 5 239 0 1978 0 2250 1 
COMMENT             3665 0 3965 1 0 "") 
COMMENT        #42=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 3 38 1 828 0 1440 
COMMENT             1 0 "") 
COMMENT        #43=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 1 4266 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BfaI" "ctag" 2 0 6 46 0 716 0 887 0 2225 0 
COMMENT             2695 0 3971 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 2 3194 0 3460 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 4 357 0 2193 0 3772 0 
COMMENT             4154 0 0 "") #51=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 142 0 2673 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BmtI" "gctagc" 6 0 2 49 0 719 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 2 759 0 861 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BpmI" "ctggag" 23 21 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 1 3363 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 6 562 1 2144 0 2548 1 
COMMENT             3840 0 4081 1 4379 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 1 2479 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 1 2585 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 4 64 0 247 0 1323 0 1809 
COMMENT             0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 1856 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 13 489 0 816 0 1224 0 
COMMENT             1326 0 1327 0 1429 0 1704 0 1986 0 2008 0 2523 0 3217 0 3619 0 4304 
COMMENT             0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 4 632 0 3481 0 4111 0 
COMMENT             4258 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 2 1682 1 1982 1 0 
COMMENT             "") 
COMMENT        #65=(CRSite (CStringList) "BscAI" "gcatc" 10 12 9 108 0 276 0 2965 1 
COMMENT             3049 1 3184 1 3271 1 3391 1 3702 1 4370 1 0 "") 
COMMENT        #66=(CRSite (CStringList) "BseMII" "ctcag" 16 14 11 134 0 167 1 860 0 
COMMENT             2034 1 2196 1 2487 1 2562 1 2677 1 3377 0 3772 1 4181 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BseRI" "gaggag" 17 15 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BseYI" "cccagc" 2 6 2 2700 0 4160 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 853 0 1323 1 2693 0 0 
COMMENT             "") 
COMMENT        #70=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 2 3346 0 4130 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 3 796 0 2193 0 4154 0 0 
COMMENT             "") #72=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 11 234 0 632 0 1818 0 
COMMENT             2271 0 3260 0 3592 0 3609 0 3986 0 4265 0 4431 0 4449 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 6 1132 0 1856 1 1937 1 
COMMENT             2519 0 2829 0 3362 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 1937 1 3362 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsmFI" "gggac" 16 20 7 75 0 1315 0 1346 0 
COMMENT             1424 0 2364 0 2667 1 2670 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 3230 1 3307 1 0 "") 
COMMENT        #78=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 7 357 0 796 0 2193 0 
COMMENT             2665 0 3001 0 3772 0 4154 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 2 791 1 3770 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 11 133 0 168 1 859 0 
COMMENT             2035 1 2197 1 2488 1 2563 1 2678 1 3376 0 3773 1 4182 1 0 "") 
COMMENT        #81=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 632 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 2143 0 2870 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 1848 0 2599 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 1 200 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 422 0 2064 1 2736 1 
COMMENT             2844 1 0 "") 
COMMENT        #86=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 1 3300 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 3 441 0 786 0 2711 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "BsrI" "actgg" 7 5 11 137 0 487 0 968 1 1105 0 
COMMENT             1351 1 1952 1 2679 1 3137 0 3937 0 4049 1 4062 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 1 2453 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BssSI" "cacgag" 2 6 1 4291 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 1043 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 12 288 1 304 0 964 1 1387 
COMMENT             0 1429 0 1714 0 2004 1 2018 0 2053 0 2799 1 2977 0 3603 1 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstKTI" "gatc" 4 0 13 56 0 488 0 691 0 1557 0 
COMMENT             2211 0 2361 0 3216 0 3346 0 3598 0 3807 0 3815 0 3826 0 3901 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 14 97 0 265 0 751 0 979 0 
COMMENT             987 0 1328 0 1480 0 1706 0 2639 0 3236 0 3593 0 4305 0 4318 0 4439 
COMMENT             0 0 "") 
COMMENT        #97=(CRSite (CStringList) "BstUI" "cgcg" 3 0 12 1421 0 1516 0 1847 0 
COMMENT             2438 0 2453 0 2455 0 2579 0 2953 0 3003 0 3348 0 3840 0 4421 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 6 53 0 485 0 2358 0 3595 
COMMENT             0 3812 0 3823 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 3 489 0 816 0 2008 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "BthCI" "gcngc" 5 0 16 668 0 1516 0 1585 0 
COMMENT              2107 0 2294 0 2608 0 2677 0 2809 0 2812 0 2953 0 3852 0 4058 0 
COMMENT              4061 0 4126 0 4269 0 4424 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 883 0 3207 1 3294 0 0 
COMMENT              "") 
COMMENT        #105=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 18 47 0 240 0 251 0 454 
COMMENT              0 670 0 717 0 1406 0 1453 0 1633 0 1845 0 2260 0 2455 0 3001 0 
COMMENT              3285 0 3431 0 3854 0 4414 0 4451 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "CdiI" "catcg" 5 5 3 2092 0 3055 1 3715 1 0 
COMMENT              "") 
COMMENT        #107=(CRSite (CStringList) "ChaI" "gatc" 5 0 13 57 0 489 0 692 0 1558 0 
COMMENT              2212 0 2362 0 3217 0 3347 0 3599 0 3808 0 3816 0 3827 0 3902 0 0 
COMMENT              "") 
COMMENT        #108=(CRSite (CStringList) "CjeI" "ccannnnnngt" 27 21 12 676 0 824 0 
COMMENT              1049 1 1310 0 1415 0 1454 0 1509 0 3803 0 3867 1 3903 0 3981 0 
COMMENT              4422 1 0 "") 
COMMENT        #109=(CRSite (CStringList) "CjePI" "ccannnnnnntc" 27 21 8 824 0 981 1 
COMMENT              1504 0 2577 0 3179 1 3431 1 3803 0 3916 1 0 "") 
COMMENT        #110=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "Csp6I" "gtac" 2 0 12 442 0 787 0 908 0 1315 
COMMENT              0 1357 0 1399 0 1438 0 2243 0 2305 0 2381 0 2712 0 3180 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "CviAII" "catg" 2 0 26 50 0 490 0 669 0 767 0 
COMMENT              772 0 817 0 943 0 1441 0 1766 0 1867 0 2009 0 2144 0 2218 0 2248 0 
COMMENT              2259 0 2297 0 2384 0 2419 0 2491 0 2646 0 2871 0 2968 0 3080 0 
COMMENT              3195 0 3500 0 4465 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "CviJI" "rgcy" 3 0 65 119 0 137 0 226 0 287 0 
COMMENT              303 0 349 0 355 0 456 0 494 0 501 0 522 0 665 0 715 0 794 0 869 0 
COMMENT              895 0 1051 0 1085 0 1135 0 1207 0 1249 0 1483 0 1653 0 1770 0 1791 
COMMENT              0 1936 0 1965 0 2086 0 2094 0 2104 0 2138 0 2228 0 2283 0 2316 0 
COMMENT              2570 0 2605 0 2663 0 2677 0 2704 0 2806 0 2812 0 2818 0 2925 0 
COMMENT              2950 0 2999 0 3052 0 3136 0 3429 0 3433 0 3652 0 3770 0 3907 0 
COMMENT              3936 0 3979 0 3990 0 4055 0 4134 0 4159 0 4164 0 4210 0 4300 0 
COMMENT              4398 0 4424 0 4442 0 4453 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "DdeI" "ctnag" 2 0 15 75 0 120 0 176 0 276 0 
COMMENT              704 0 846 0 2043 0 2100 0 2205 0 2496 0 2571 0 2686 0 3363 0 3781 
COMMENT              0 4190 0 0 "") 
COMMENT        #115=(CRSite (CStringList) "DpnI" "gatc" 3 0 13 55 0 487 0 690 0 1556 0 
COMMENT              2210 0 2360 0 3215 0 3345 0 3597 0 3806 0 3814 0 3825 0 3900 0 0 
COMMENT              "") 
COMMENT        #116=(CRSite (CStringList) "DraI" "tttaaa" 4 0 2 462 0 570 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 1 4362 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "EaeI" "yggccr" 2 0 3 492 0 663 0 2092 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "EagI" "cggccg" 2 0 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "EarI" "ctcttc" 8 11 6 330 0 521 1 1218 0 
COMMENT              1604 0 1754 0 3159 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "EciI" "ggcgga" 18 16 4 180 1 2438 0 4262 0 
COMMENT              4408 0 0 "") 
COMMENT        #123=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 3 593 1 1706 0 3922 
COMMENT              1 0 "") 
COMMENT        #124=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 6 193 0 226 0 1377 0 2361 
COMMENT              0 3217 0 4085 0 0 "") 
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COMMENT                                             1))))) "Bpu10I: 1 site") 2 5 
COMMENT                             "CCTNAGC" "GGANTCG") 
COMMENT                       #400=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722424 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BpuEI: 6 sites") 22 20 
COMMENT                             "CTTGAGNNNNNNNNNNNNNNNNN" "GAACTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #401=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 47722552 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CObjectList 
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COMMENT                                             (CParagraph 0 (1 #58# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2479 " 1))))) 
COMMENT                              "BsaAI: 1 site") 3 3 "YACGTR" "RTGCAY") 
COMMENT                       #404=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722456 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #405=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #59# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 2585 " 1))))) 
COMMENT                              "BsaBI: 1 site") 5 5 "GATNNNNATC" "CTANNNNTAG") 
COMMENT                       #407=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722488 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaHI: 4 sites") 2 4 "GRCGYC" 
COMMENT                             "CYGCRG") 
COMMENT                       #408=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722360 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #409=(CGroupPar 
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COMMENT                                      (CObjectList 
COMMENT                                       #410=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 1856 (on complementary strand)" 
COMMENT                                             1))))) "BsaI: 1 site") 7 11 
COMMENT                             "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                       #411=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722392 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaJI: 13 sites") 1 5 "CCNNGG" 
COMMENT                             "GGNNCC") 
COMMENT                       #412=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722264 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaWI: 4 sites") 1 5 "WCCGGW" 
COMMENT                             "WGGCCW") 
COMMENT                       #413=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722328 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #414=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #64# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #415=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #64# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 1682 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #416=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #64# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 1982 (on complementary strand)" 
COMMENT                                             1))))) "BsaXI: 2 sites") 21 18 
COMMENT                             "ACNNNNNCTCCNNNNNNNNNNN" "TGNNNNNGAGGNNNNNNNNNNN") 
COMMENT                       #417=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722296 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BscAI: 9 sites") 9 11 
COMMENT                             "GCATCNNNNNNN" "CGTAGNNNNNNN") 
COMMENT                       #418=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722680 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseMII: 11 sites") 15 13 
COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
COMMENT                       #419=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722712 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseRI: 0 sites") 16 14 
COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
COMMENT                       #420=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 47722232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #421=(CGroupPar 
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COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 2700 " 1) 
COMMENT                                       #423=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #68# 2) 1 2 2 0 
COMMENT                                              191) " N2: 4160 " 1))))) 
COMMENT                              "BseYI: 2 sites") 1 5 "CCCAGC" "GGGTCG") 
COMMENT                       #424=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722616 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsgI: 3 sites") 22 20 
COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #425=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722200 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #426=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #70# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #427=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #70# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3346 " 1) 
COMMENT                                       #428=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #70# 2) 1 2 2 0 
COMMENT                                              191) " N2: 4130 " 1))))) 
COMMENT                              "BsiEI: 2 sites") 4 2 "CGRYCG" "GCYRGC") 
COMMENT                       #429=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722808 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiHKAI: 3 sites") 5 1 "GWGCWC" 
COMMENT                             "CWCGWG") 
COMMENT                       #430=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722776 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiWI: 0 sites") 1 5 "CGTACG" 
COMMENT                             "GCATGC") 
COMMENT                       #431=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722936 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BslI: 11 sites") 7 4 
COMMENT                             "CCNNNNNNNGG" "GGNNNNNNNCC") 
COMMENT                       #432=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47722872 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmAI: 6 sites") 6 10 
COMMENT                             "GTCTCNNNNNN" "CAGAGNNNNNN") 
COMMENT                       #433=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723032 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #434=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #75# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #435=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #75# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 1937 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #436=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #75# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3362 " 1))))) 
COMMENT                              "BsmBI: 2 sites") 7 11 "CGTCTCNNNNNN" 
COMMENT                             "GCAGAGNNNNNN") 
COMMENT                       #437=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723000 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmFI: 7 sites") 15 19 
COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
COMMENT                       #438=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723096 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #439=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #77# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #440=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #77# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 3230 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #441=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #77# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 3307 (on complementary strand)" 
COMMENT                                             1))))) "BsmI: 2 sites") 7 5 
COMMENT                             "GAATGCNN" "CTTACGNN") 
COMMENT                       #442=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56514760 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsp1286I: 7 sites") 5 1 "GDGCHC" 
COMMENT                             "CHCGDG") 
COMMENT                       #443=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 56514800 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #444=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #79# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #445=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 791 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #446=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 3770 (on complementary strand)" 
COMMENT                                             1))))) "Bsp24I: 2 sites") 24 19 
COMMENT                             "GACNNNNNNTGGNNNNNNNNNNNNN" 
COMMENT                             "CTGNNNNNNACCNNNNNNNNNNNNN") 
COMMENT                       #447=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723064 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspCNI: 11 sites") 14 12 
COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
COMMENT                       #448=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723192 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #449=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #81# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #450=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #81# 1) 1 2 2 0 
COMMENT                                              191) " N1: 632 " 1))))) 
COMMENT                              "BspEI: 1 site") 1 5 "TCCGGA" "AGGCCT") 
COMMENT                       #451=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723128 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #452=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #82# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #453=(CLinePar 
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COMMENT                                              191) " N1: 2143 " 1) 
COMMENT                                       #454=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #82# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2870 " 1))))) 
COMMENT                              "BspHI: 2 sites") 1 5 "TCATGA" "AGTACT") 
COMMENT                       #455=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723256 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #456=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #83# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #457=(CLinePar 
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COMMENT                                              191) " N1: 1848 " 1) 
COMMENT                                       #458=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #83# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 2599 (on complementary strand)" 
COMMENT                                             1))))) "BspMI: 2 sites") 10 14 
COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
COMMENT                       #459=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723224 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #460=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #84# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #461=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #84# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 200 (on complementary strand)" 
COMMENT                                             1))))) "BsrBI: 1 site") 3 3 
COMMENT                             "CCGCTC" "GGCGAG") 
COMMENT                       #462=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723320 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrDI: 4 sites") 8 6 "GCAATGNNN" 
COMMENT                             "CGTTACNNN") 
COMMENT                       #463=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723288 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #464=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #86# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #465=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #86# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3300 " 1))))) 
COMMENT                              "BsrFI: 1 site") 1 5 "RCCGGY" "YGGCCR") 
COMMENT                       #466=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723512 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrGI: 3 sites") 1 5 "TGTACA" 
COMMENT                             "ACATGT") 
COMMENT                       #467=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723352 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsrI: 11 sites") 6 4 "ACTGGNN" 
COMMENT                             "TGACCNN") 
COMMENT                       #468=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723384 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #469=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #89# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #470=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #89# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2453 " 1))))) 
COMMENT                              "BssHII: 1 site") 1 5 "GCGCGC" "CGCGCG") 
COMMENT                       #471=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723448 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #472=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #90# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #473=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #90# 1) 1 2 2 0 
COMMENT                                              191) " N1: 4291 " 1))))) 
COMMENT                              "BssSI: 1 site") 1 5 "CACGAG" "GTGCTC") 
COMMENT                       #474=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723480 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstAPI: 0 sites") 7 4 
COMMENT                             "GCANNNNNTGC" "CGTNNNNNACG") 
COMMENT                       #475=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723416 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstBI: 0 sites") 2 4 "TTCGAA" 
COMMENT                             "AAGCTT") 
COMMENT                       #476=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723576 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #477=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #93# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #478=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #93# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1043 " 1))))) 
COMMENT                              "BstEII: 1 site") 1 6 "GGTNACC" "CCANTGG") 
COMMENT                       #479=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723544 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstF5I: 12 sites") 7 5 "GGATGNNN" 
COMMENT                             "CCTACNNN") 
COMMENT                       #480=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723640 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstKTI: 13 sites") 3 1 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #481=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723608 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstNI: 14 sites") 2 3 "CCWGG" 
COMMENT                             "GGWCC") 
COMMENT                       #482=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723672 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstUI: 12 sites") 2 2 "CGCG" 
COMMENT                             "GCGC") 
COMMENT                       #483=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47723704 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstXI: 0 sites") 8 4 
COMMENT                             "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                       #484=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719640 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstYI: 6 sites") 1 5 "RGATCY" 
COMMENT                             "YCTAGR") 
COMMENT                       #485=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47724728 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstZ17I: 0 sites") 3 3 "GTATAC" 
COMMENT                             "CATATG") 
COMMENT                       #486=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719448 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsu36I: 0 sites") 2 5 "CCTNAGG" 
COMMENT                             "GGANTCC") 
COMMENT                       #487=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719416 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtgI: 3 sites") 1 5 "CCRYGG" 
COMMENT                             "GGYRCC") 
COMMENT                       #488=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719352 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BthCI: 16 sites") 4 1 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #489=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719320 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtsI: 3 sites") 8 6 "GCAGTGNNN" 
COMMENT                             "CGTCACNNN") 
COMMENT                       #490=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719032 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Cac8I: 18 sites") 3 3 "GCNNGC" 
COMMENT                             "CGNNCG") 
COMMENT                       #491=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47719000 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CdiI: 3 sites") 4 4 "CATCG" 
COMMENT                             "GTAGC") 
COMMENT                       #492=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718968 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ChaI: 13 sites") 5 1 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #493=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718936 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjeI: 12 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNCANNNNNNNNNNNNNNNN") 
COMMENT                       #494=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718904 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjePI: 8 sites") 26 20 
COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNNAGNNNNNNNNNNNNNNN") 
COMMENT                       #495=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718872 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                             "TAGCTA") 
COMMENT                       #496=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718840 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Csp6I: 12 sites") 1 3 "GTAC" 
COMMENT                             "CATG") 
COMMENT                       #497=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718808 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviAII: 26 sites") 1 3 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #498=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718776 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviJI: 65 sites") 2 2 "RGCY" 
COMMENT                             "YCGR") 
COMMENT                       #499=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718744 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DdeI: 15 sites") 1 4 "CTNAG" 
COMMENT                             "GANTC") 
COMMENT                       #500=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718648 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DpnI: 13 sites") 2 2 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #501=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718616 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #502=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #116# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #503=(CLinePar 
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COMMENT                                              191) " N1: 462 " 1) 
COMMENT                                       #504=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #116# 2) 1 2 2 0 
COMMENT                                              191) " N2: 570 " 1))))) 
COMMENT                              "DraI: 2 sites") 3 3 "TTTAAA" "AAATTT") 
COMMENT                       #505=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718584 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DraIII: 0 sites") 6 3 "CACNNNGTG" 
COMMENT                             "GTGNNNCAC") 
COMMENT                       #506=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 47718552 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #507=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #118# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #508=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #118# 1) 1 2 2 0 
COMMENT                                              191) " N1: 4362 " 1))))) 
COMMENT                              "DrdI: 1 site") 7 5 "GACNNNNNNGTC" "CTGNNNNNNCAG") 
COMMENT                       #509=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     misc_recomb     358..457
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     misc_recomb     complement(2698..2797)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     CDS             2920..3729
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      3793..4466
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     CDS             490..2685
                     /vntifkey="4"
                     /label=NvPCASPt1B
     misc_feature    2686..2688
                     /vntifkey="21"
                     /label=stop
     misc_feature    458..459
                     /vntifkey="21"
                     /label=NN
     CDS             460..489
                     /vntifkey="4"
                     /label=random\translated\sequence\(if\N-fusion)
BASE COUNT     1192 a      1034 c      1098 g      1145 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatcca tggccgacag cttgtgtatt atactgtttg gcttgaagag gaatattcaa 
      541 tttagagttt ctaatgactt tctgctttta aaagactcaa gaaatatccc gaaggatttg 
      601 tcttcgcttc agcaaaaatt cactccacta tccggattta cagtcaggtc cattaggagt 
      661 atggccgcat gctgtccaac aatctctgat cttccaaaag ttctaagaga gaagctagca 
      721 catattcttg atgagagcaa tactcctacc tggcgggaac taatccatgt catgccacca 
      781 gatatgtaca gagctcacca agtcgagtca tttgccatgg caaccttgaa gtgcaggagt 
      841 cctactcagg aattatttga tgatttggct cgacgcagtg tcacactaga tgagctatca 
      901 ggttgggtac agaaactgcc acaaagcaat aaaaagcagt ccatgattca acttctatat 
      961 ggacatccag ttgttaccag gcaaccagga aatataatgg ttgctgtagg agcaaatgct 
     1021 gtgctatcgt gtggtgcaac aggtcaccag cctctcaact accaatggtt taagagcaag 
     1081 aaagctttag aagggcaaac tggttccctc cttcgtgtta agaatgtctc caagcttgat 
     1141 gaggggcatt acatatgtcg tgttgcaaac tctaatggcg actatgtgtt tactgaatgg 
     1201 gcaaagctat ctcttcaaaa tccctcggaa acacatcagt tcagtcggcc tatcatcaca 
     1261 agtcaacccg aatacccaga agtccatact gtgttaggtg ggacgcttcg cctgtactgc 
     1321 gacgccctgg gggaccctgc accaactttc cagtggtaca agaatgacct tcccctcccg 
     1381 gatggcgacc aaagggagta ctgcaagcgg gacatttgcg cggatgacca aggaaggtac 
     1441 atgtgcgtgg caagcaactc gtctgggtcc actgaatcca ggccaactcg agtgtttgtc 
     1501 agacatcagg tgccgcgtgg tcaaatggaa gcaaatgaca aggtggcgtt actgatcggc 
     1561 aacaaagatt acaaaatggc gcagcacctc aacactctct tccaccctct gaatgatgtg 
     1621 tgcgacttgg caggcgtatt aagaagcata ggctttaagg ttgtttcgct tgtgaatctc 
     1681 actctgaagg agatggagtt cgccctggat gagttctaca aactgctggt caaagacacc 
     1741 tatgcgctct tctactttgc gggtcatggc tttgagataa atggacaggg ctatcttatg 
     1801 cctatagacg ccacagaaag gtatcattcg caggaaaacc tgcccgcgtg gaaagtgttg 
     1861 gagaccatgc agaatagcga agcaaaacta aatattatcg tcctcgattg ctgtcgaaca 
     1921 acgccaaacg ataagccaat cgagacgata ccagtcgaca acggctgtta ccaaccgatt 
     1981 gtaaccaagg agaatgtgtt ggtcatccat ggatgtttac cgcagaattc agtattcgag 
     2041 tctgaggatg agagaaatgg tttcattgcc aagcacctca tcaagctcat cggccaaact 
     2101 aagccgcttt ccaagattaa acttgagttc tcaaaagcca ttcatgatga gaaaatttat 
     2161 gaccccgaag atagaaaaca acaagtcgtg cacattcata ccactgagat cgacgacatg 
     2221 tgcctagctg acaatacagc ggtacacatg caaggagcat gcgccgtgtc tgggagcgag 
     2281 ggctggaaag cagcgcatgg tcggtacaca cagggcttcc gttattttcg ttccagcggg 
     2341 ggaggggggg gaccagagat cccggaagac cccgtgaccg tacatgagcg tgacggggtc 
     2401 gtggtcgaac tcgtcttcat ggcggaattc tccaacgcgt tgctcgtcac cgcgcgctgt 
     2461 cgccccgaca actaccacgt gacattcgac atgcctgagt gtgtcagcgg ttgtctcgtt 
     2521 accaaggtaa cgggcaagga aagcactatt ccacagcgtt tctcaagagc tgagtccgcg 
     2581 atacgaatcg ctgaccttca aaggctgcag gttttcctgt gcttgaatat ggaccgccag 
     2641 gaagcatggg gtctgggacg agcccatata gtgcagccca gtccctgaga tatctagacc 
     2701 cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 
     2761 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca gctctggccc 
     2821 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata 
     2881 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa 
     2941 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 
     3001 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg 
     3061 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 
     3121 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 
     3181 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta 
     3241 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 
     3301 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 
     3361 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 
     3421 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 
     3481 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 
     3541 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 
     3601 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 
     3661 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 
     3721 tttttctaat cagaattggt taattggttg taacattatt cagattgggc cccgttccac 
     3781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     3841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     3901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     3961 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     4021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     4081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     4141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     4201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     4261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     4321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     4381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     4441 gccttttgct ggccttttgc tcacatgtt 
//

