LOCUS       pENTR3C-hPKCepsilon      4504 bp    DNA     circular UNA 09-NOV-2011
DEFINITION  LMBP 9923.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_recomb     complement(11..110)
                     /label=attL2
     CDS             233..1042
                     /label=Kan(R)
     rep_origin      1106..1779
                     /label="pUC origin"
     terminator      complement(1888..1931)
                     /label="rrnB T1 transcription terminator"
     terminator      complement(2063..2090)
                     /label="rrnB T2 transcription terminator"
     misc_recomb     2140..2239
                     /label=attL1
     CDS             2287..4500
                     /note="hPKCepsilon CDS"
                     /label=hPKCepsilon
ORIGIN
        1 AGATATCTAG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA
       61 ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT
      121 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC
      181 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA
      241 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG
      301 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG
      361 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT
      421 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA
      481 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC
      541 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC
      601 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG
      661 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA
      721 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT
      781 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT
      841 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG
      901 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA
      961 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT
     1021 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACATT ATTCAGATTG
     1081 GGCCCCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     1141 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     1201 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     1261 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     1321 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     1381 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     1441 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     1501 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     1561 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     1621 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     1681 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     1741 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     1801 TGATTCTGTG GATAACCGTA TTACCGCTAG CATGGATCTC GGGGACGTCT AACTACTAAG
     1861 CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA AGACTGGGCC
     1921 TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA
     1981 GCGGATTTGA ACGTTGTGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA
     2041 ACTGCCAGGC ATCAAACTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA
     2101 CAAACTCTTC CTGTTAGTTA GTTACTTAAG CTCGGGCCCC AAATAATGAT TTTATTTTGA
     2161 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATAAGCAAT GCTTTTTTAT AATGCCAACT
     2221 TTGTACAAAA AAGCAGGCTC TTTAAAGGAA CCAATTCAGT CGACTGGATC CGGTACCGAA
     2281 TTCACCATGG TAGTGTTCAA TGGCCTTCTT AAGATCAAAA TCTGCGAGGC CGTGAGCTTG
     2341 AAGCCCACAG CCTGGTCGCT GCGCCATGCG GTGGGACCCC GGCCGCAGAC TTTCCTTCTC
     2401 GACCCCTACA TTGCCCTCAA TGTGGACGAC TCGCGCATCG GCCAAACGGC CACCAAGCAG
     2461 AAGACCAACA GCCCGGCCTG GCACGACGAG TTCGTCACCG ATGTGTGCAA CGGACGCAAG
     2521 ATCGAGCTGG CTGTCTTTCA CGATGCCCCC ATAGGCTACG ACGACTTCGT GGCCAACTGC
     2581 ACCATCCAGT TTGAGGAGCT GCTGCAGAAC GGGAGCCGCC ACTTCGAGGA CTGGATTGAT
     2641 CTGGAGCCAG AAGGAAGAGT GTATGTGATC ATCGATCTCT CAGGGTCGTC GGGTGAAGCC
     2701 CCTAAAGACA ATGAAGAGCG TGTGTTCAGG GAACGCATGC GGCCGAGGAA GCGGCAGGGG
     2761 GCCGTCAGGC GCAGGGTCCA TCAGGTCAAC GGCCACAAGT TCATGGCCAC CTATCTTCGG
     2821 CAGCCCACCT ACTGCTCCCA TTGCAGAGAC TTCATCTGGG GTGTCATAGG AAAGCAGGGA
     2881 TACCAGTGTC AAGTCTGCAC CTGCGTGGTC CACAAGCGGT GCCACGAGCT CATAATCACA
     2941 AAGTGTGCTG GGTTAAAGAA GCAGGAGACC CCCGACCAGG TGGGCTCCCA GCGGTTCAGC
     3001 GTCAACATGC CCCACAAGTT CGGTATCCAC AACTACAAGG TCCCTACCTT CTGCGATCAC
     3061 TGTGGGTCCC TGCTCTGGGG ACTCTTGCGG CAGGGTTTGC AGTGTAAAGT CTGCAAAATG
     3121 AATGTTCACC GTCGATGTGA GACCAACGTG GCTCCCAACT GTGGAGTGGA TGCCAGAGGA
     3181 ATCGCCAAAG TACTGGCCGA CCTGGGCGTT ACCCCAGACA AAATCACCAA CAGCGGCCAG
     3241 AGAAGGAAAA AGCTCATTGC TGGTGCCGAG TCCCCGCAGC CTGCTTCTGG AAGCTCACCA
     3301 TCTGAGGAAG ATCGATCCAA GTCAGCACCC ACCTCCCCTT GTGACCAGGA AATAAAAGAA
     3361 CTTGAGAACA ACATTCGGAA AGCCTTGTCA TTTGACAACC GAGGAGAGGA GCACCGGGCA
     3421 GCATCGTCTC CTGATGGCCA GCTGATGAGC CCCGGTGAGA ATGGCGAAGT CCGGCAAGGC
     3481 CAGGCCAAGC GCCTGGGCCT GGATGAGTTC AACTTCATCA AGGTGTTGGG CAAAGGCAGC
     3541 TTTGGCAAGG TCATGTTGGC AGAACTCAAG GGCAAAGATG AAGTATATGC TGTGAAGGTC
     3601 TTAAAGAAGG ACGTCATCCT TCAGGATGAT GACGTGGACT GCACAATGAC AGAGAAGAGG
     3661 ATTTTGGCTC TGGCACGGAA ACACCCGTAC CTTACCCAAC TCTACTGCTG CTTCCAGACC
     3721 AAGGACCGCC TCTTTTTCGT CATGGAATAT GTAAATGGTG GAGACCTCAT GTTTCAGATT
     3781 CAGCGCTCCC GAAAATTCGA CGAGCCTCGT TCACGGTTCT ATGCTGCAGA GGTCACATCG
     3841 GCCCTCATGT TCCTCCACCA GCATGGAGTC ATCTACAGGG ATTTGAAACT GGACAACATC
     3901 CTTCTGGATG CAGAAGGTCA CTGCAAGCTG GCTGACTTCG GGATGTGCAA GGAAGGGATT
     3961 CTGAATGGTG TGACGACCAC CACGTTCTGT GGGACTCCTG ACTACATAGC TCCTGAGATC
     4021 CTGCAGGAGT TGGAGTATGG CCCCTCCGTG GACTGGTGGG CCCTGGGGGT GCTGATGTAC
     4081 GAGATGATGG CTGGACAGCC TCCCTTTGAG GCCGACAATG AGGACGACCT ATTTGAGTCC
     4141 ATCCTCCATG ACGACGTGCT GTACCCAGTC TGGCTCAGCA AGGAGGCTGT CAGCATCTTG
     4201 AAAGCTTTCA TGACGAAGAA TCCCCACAAG CGCCTGGGCT GTGTGGCATC GCAGAATGGC
     4261 GAGGACGCCA TCAAGCAGCA CCCATTCTTC AAAGAGATTG ACTGGGTGCT CCTGGAGCAG
     4321 AAGAAGATCA AGCCACCCTT CAAACCACGC ATTAAAACCA AAAGAGACGT CAATAATTTT
     4381 GACCAAGACT TTACCCGGGA AGAGCCGGTA CTCACCCTTG TGGACGAAGC AATTGTAAAG
     4441 CAGATCAACC AGGAGGAATT CAAAGGTTTC TCCTACTTTG GTGAAGACCT GATGCCCTGA
     4501 CTCG
//

