LOCUS       pENTR3C-hPKCbeta2-no_stop      4311 bp    DNA     circular UNA 09-NOV-2011
DEFINITION  LMBP 9932.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_recomb     complement(11..110)
                     /label=attL2
     CDS             233..1042
                     /label=Kan(R)
     rep_origin      1106..1779
                     /label="pUC origin"
     terminator      complement(1888..1931)
                     /label="rrnB T1 transcription terminator"
     terminator      complement(2063..2090)
                     /label="rrnB T2 transcription terminator"
     misc_recomb     2140..2239
                     /label=attL1
     CDS             2287..4305
                     /label="hPKCbeta2 ns"
ORIGIN
        1 AGATATCTAG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA
       61 ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT
      121 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC
      181 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA
      241 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG
      301 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG
      361 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT
      421 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA
      481 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC
      541 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC
      601 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG
      661 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA
      721 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT
      781 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT
      841 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG
      901 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA
      961 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT
     1021 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACATT ATTCAGATTG
     1081 GGCCCCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     1141 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     1201 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     1261 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     1321 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     1381 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     1441 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     1501 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     1561 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     1621 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     1681 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     1741 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     1801 TGATTCTGTG GATAACCGTA TTACCGCTAG CATGGATCTC GGGGACGTCT AACTACTAAG
     1861 CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA AGACTGGGCC
     1921 TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA
     1981 GCGGATTTGA ACGTTGTGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA
     2041 ACTGCCAGGC ATCAAACTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA
     2101 CAAACTCTTC CTGTTAGTTA GTTACTTAAG CTCGGGCCCC AAATAATGAT TTTATTTTGA
     2161 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATAAGCAAT GCTTTTTTAT AATGCCAACT
     2221 TTGTACAAAA AAGCAGGCTC TTTAAAGGAA CCAATTCAGT CGACTGGATC CGGTACCGAA
     2281 TTCACCATGG CTGACCCGGC TGCGGGGCCG CCGCCGAGCG AGGGCGAGGA GAGCACCGTG
     2341 CGCTTCGCCC GCAAAGGCGC CCTCCGGCAG AAGAACGTGC ATGAGGTCAA GAACCACAAA
     2401 TTCACCGCCC GCTTCTTCAA GCAGCCCACC TTCTGCAGCC ACTGCACCGA CTTCATCTGG
     2461 GGCTTCGGGA AGCAGGGATT CCAGTGCCAA GTTTGCTGCT TTGTGGTGCA CAAGCGGTGC
     2521 CATGAATTTG TCACATTCTC CTGCCCTGGC GCTGACAAGG GTCCAGCCTC CGATGACCCC
     2581 CGCAGCAAAC ACAAGTTTAA GATCCACACG TACTCCAGCC CCACGTTTTG TGACCACTGT
     2641 GGGTCACTGC TGTATGGACT CATCCACCAG GGGATGAAAT GTGACACCTG CATGATGAAT
     2701 GTGCACAAGC GCTGCGTGAT GAATGTTCCC AGCCTGTGTG GCACGGACCA CACGGAGCGC
     2761 CGCGGCCGCA TCTACATCCA GGCCCACATC GACAGGGACG TCCTCATTGT CCTCGTAAGA
     2821 GATGCTAAAA ACCTTGTACC TATGGACCCC AATGGCCTGT CAGATCCCTA CGTAAAACTG
     2881 AAACTGATTC CCGATCCCAA AAGTGAGAGC AAACAGAAGA CCAAAACCAT CAAATGCTCC
     2941 CTCAACCCTG AGTGGAATGA GACATTTAGA TTTCAGCTGA AAGAATCGGA CAAAGACAGA
     3001 AGACTGTCAG TAGAGATTTG GGATTGGGAT TTGACCAGCA GGAATGACTT CATGGGATCT
     3061 TTGTCCTTTG GGATTTCTGA ACTTCAGAAA GCCAGTGTTG ATGGCTGGTT TAAGTTACTG
     3121 AGCCAGGAGG AAGGCGAGTA CTTCAATGTG CCTGTGCCAC CAGAAGGAAG TGAGGCCAAT
     3181 GAAGAACTGC GGCAGAAATT TGAGAGGGCC AAGATCAGTC AGGGAACCAA GGTCCCGGAA
     3241 GAAAAGACGA CCAACACTGT CTCCAAATTT GACAACAATG GCAACAGAGA CCGGATGAAA
     3301 CTGACCGATT TTAACTTCCT AATGGTGCTG GGGAAAGGCA GCTTTGGCAA GGTCATGCTT
     3361 TCAGAACGAA AAGGCACAGA TGAGCTCTAT GCTGTGAAGA TCCTGAAGAA GGACGTTGTG
     3421 ATCCAAGATG ATGACGTGGA GTGCACTATG GTGGAGAAGC GGGTGTTGGC CCTGCCTGGG
     3481 AAGCCGCCCT TCCTGACCCA GCTCCACTCC TGCTTCCAGA CCATGGACCG CCTGTACTTT
     3541 GTGATGGAGT ACGTGAATGG GGGCGACCTC ATGTATCACA TCCAGCAAGT CGGCCGGTTC
     3601 AAGGAGCCCC ATGCTGTATT TTACGCTGCA GAAATTGCCA TCGGTCTGTT CTTCTTACAG
     3661 AGTAAGGGCA TCATTTACCG TGACCTAAAA CTTGACAACG TGATGCTCGA TTCTGAGGGA
     3721 CACATCAAGA TTGCCGATTT TGGCATGTGT AAGGAAAACA TCTGGGATGG GGTGACAACC
     3781 AAGACATTCT GTGGCACTCC AGACTACATC GCCCCCGAGA TAATTGCTTA TCAGCCCTAT
     3841 GGGAAGTCCG TGGATTGGTG GGCATTTGGA GTCCTGCTGT ATGAAATGTT GGCTGGGCAG
     3901 GCACCCTTTG AAGGGGAGGA TGAAGATGAA CTCTTCCAAT CCATCATGGA ACACAACGTA
     3961 GCCTATCCCA AGTCTATGTC CAAGGAAGCT GTGGCCATCT GCAAAGGGCT GATGACCAAA
     4021 CACCCAGGCA AACGTCTGGG TTGTGGACCT GAAGGCGAAC GTGATATCAA AGAGCATGCA
     4081 TTTTTCCGGT ATATTGATTG GGAGAAACTT GAACGCAAAG AGATCCAGCC CCCTTATAAG
     4141 CCAAAAGCTT GTGGGCGAAA TGCTGAAAAC TTCGACCGAT TTTTCACCCG CCATCCACCA
     4201 GTCCTAACAC CTCCCGACCA GGAAGTCATC AGGAATATTG ACCAATCAGA ATTCGAAGGA
     4261 TTTTCCTTTG TTAACTCTGA ATTTTTAAAA CCCGAAGTCA AGAGCCACTC G
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