LOCUS       pENTR3C-hPKCiota-no_stop      4080 bp    DNA     circular UNA 09-NOV-2011
DEFINITION  LMBP 9937.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_recomb     complement(11..110)
                     /label=attL2
     CDS             233..1042
                     /label=Kan(R)
     rep_origin      1106..1779
                     /label="pUC origin"
     terminator      complement(1888..1931)
                     /label="rrnB T1 transcription terminator"
     terminator      complement(2063..2090)
                     /label="rrnB T2 transcription terminator"
     misc_recomb     2140..2239
                     /label=attL1
     CDS             2287..4074
                     /note="CDS"
                     /label="hPKClambdaIota ns"
ORIGIN
        1 AGATATCTAG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA
       61 ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT
      121 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC
      181 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA
      241 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG
      301 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG
      361 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT
      421 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA
      481 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC
      541 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC
      601 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG
      661 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA
      721 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT
      781 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT
      841 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG
      901 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA
      961 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT
     1021 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACATT ATTCAGATTG
     1081 GGCCCCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     1141 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     1201 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     1261 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     1321 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     1381 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     1441 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     1501 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     1561 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     1621 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     1681 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     1741 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     1801 TGATTCTGTG GATAACCGTA TTACCGCTAG CATGGATCTC GGGGACGTCT AACTACTAAG
     1861 CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA AGACTGGGCC
     1921 TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA
     1981 GCGGATTTGA ACGTTGTGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA
     2041 ACTGCCAGGC ATCAAACTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA
     2101 CAAACTCTTC CTGTTAGTTA GTTACTTAAG CTCGGGCCCC AAATAATGAT TTTATTTTGA
     2161 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATAAGCAAT GCTTTTTTAT AATGCCAACT
     2221 TTGTACAAAA AAGCAGGCTC TTTAAAGGAA CCAATTCAGT CGACTGGATC CGGTACCGAA
     2281 TTCACCATGC CGACCCAGAG GGACAGCAGC ACCATGTCCC ACACGGTCGC AGGCGGCGGC
     2341 AGCGGGGACC ATTCCCACCA GGTCCGGGTG AAAGCCTACT ACCGCGGGGA TATCATGATA
     2401 ACACATTTTG AACCTTCCAT CTCCTTTGAG GGCCTTTGCA ATGAGGTTCG AGACATGTGT
     2461 TCTTTTGACA ACGAACAGCT CTTCACCATG AAATGGATAG ATGAGGAAGG AGACCCGTGT
     2521 ACAGTATCAT CTCAGTTGGA GTTAGAAGAA GCCTTTAGAC TTTATGAGCT AAACAAGGAT
     2581 TCTGAACTCT TGATTCATGT GTTCCCTTGT GTACCAGAAC GTCCTGGGAT GCCTTGTCCA
     2641 GGAGAAGATA AATCCATCTA CCGTAGAGGT GCACGCCGCT GGAGAAAGCT TTATTGTGCC
     2701 AATGGCCACA CTTTCCAAGC CAAGCGTTTC AACAGGCGTG CTCACTGTGC CATCTGCACA
     2761 GACCGAATAT GGGGACTTGG ACGCCAAGGA TATAAGTGCA TCAACTGCAA ACTCTTGGTT
     2821 CATAAGAAGT GCCATAAACT CGTCACAATT GAATGTGGGC GGCATTCTTT GCCACAGGAA
     2881 CCAGTGATGC CCATGGATCA GTCATCCATG CATTCTGACC ATGCACAGAC AGTAATTCCA
     2941 TATAATCCTT CAAGTCATGA GAGTTTGGAT CAAGTTGGTG AAGAAAAAGA GGCAATGAAC
     3001 ACCAGGGAAA GTGGCAAAGC TTCATCCAGT CTAGGTCTTC AGGATTTTGA TTTGCTCCGG
     3061 GTAATAGGAA GAGGAAGTTA TGCCAAAGTA CTGTTGGTTC GATTAAAAAA AACAGATCGT
     3121 ATTTATGCAA TGAAAGTTGT GAAAAAAGAG CTTGTTAATG ATGATGAGGA TATTGATTGG
     3181 GTACAGACAG AGAAGCATGT GTTTGAGCAG GCATCCAATC ATCCTTTCCT TGTTGGGCTG
     3241 CATTCTTGCT TTCAGACAGA AAGCAGATTG TTCTTTGTTA TAGAGTATGT AAATGGAGGA
     3301 GACCTAATGT TTCATATGCA GCGACAAAGA AAACTTCCTG AAGAACATGC CAGATTTTAC
     3361 TCTGCAGAAA TCAGTCTAGC ATTAAATTAT CTTCATGAGC GAGGGATAAT TTATAGAGAT
     3421 TTGAAACTGG ACAATGTATT ACTGGACTCT GAAGGCCACA TTAAACTCAC TGACTACGGC
     3481 ATGTGTAAGG AAGGATTACG GCCAGGAGAT ACAACCAGCA CTTTCTGTGG TACTCCTAAT
     3541 TACATTGCTC CTGAAATTTT AAGAGGAGAA GATTATGGTT TCAGTGTTGA CTGGTGGGCT
     3601 CTTGGAGTGC TCATGTTTGA GATGATGGCA GGAAGGTCTC CATTTGATAT TGTTGGGAGC
     3661 TCCGATAACC CTGACCAGAA CACAGAGGAT TATCTCTTCC AAGTTATTTT GGAAAAACAA
     3721 ATTCGCATAC CACGTTCTCT GTCTGTAAAA GCTGCAAGTG TTCTGAAGAG TTTTCTTAAT
     3781 AAGGACCCTA AGGAACGATT GGGTTGTCAT CCTCAAACAG GATTTGCTGA TATTCAGGGA
     3841 CACCCGTTCT TCCGAAATGT TGATTGGGAT ATGATGGAGC AAAAACAGGT GGTACCTCCC
     3901 TTTAAACCAA ATATTTCTGG GGAATTTGGT TTGGACAACT TTGATTCTCA GTTTACTAAT
     3961 GAACCTGTCC AGCTCACTCC AGATGACGAT GACATTGTGA GGAAGATTGA TCAGTCTGAA
     4021 TTTGAAGGTT TTGAGTATAT CAATCCTCTT TTGATGTCTG CAGAAGAATG TGTCCACTCG
//

