LOCUS       pBRST10                 3641 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1805.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRST10|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRST10" 0 3641 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1114129 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3641 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "5UTR" 21 0 0 52 80 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "52..80") 
COMMENT        #4=(CFSignal (CObList) "COAT" 4 0 0 81 671 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "81..671") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 0 672 1290 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "672..1290") 
COMMENT        #6=(CFSignal (CObList) "STNV" 21 0 0 52 1290 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "52..1290") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 1625 2035 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1625..2035)") 
COMMENT        #8=(CFSignal (CObList) "AMP" 4 0 1 2573 3433 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2573..3433)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pBRST10'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3641 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
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COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "coat protein of STNV" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "Start: 2573  End: 3433 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(2573..3433)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "Satellite tobacco necrosis virus" 1) 
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COMMENT                                       "   52..1290" 1))) "STNV") 
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COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of STNV RNA" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   672..1290" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (3 total)") 
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FEATURES             Location/Qualifiers
     misc_feature    52..80
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of STNV RNA"
     CDS             81..671
                     /vntifkey="4"
                     /label=COAT
                     /note="coat protein of STNV"
     misc_feature    672..1290
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of STNV RNA"
     misc_feature    52..1290
                     /vntifkey="21"
                     /label=STNV
                     /note="Satellite tobacco necrosis virus"
     rep_origin      complement(1625..2035)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2573..3433)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      943 a       905 c       908 g       885 t 
ORIGIN
        1 ttccggatcc ggccaagctt ggctctagag gctgcagggg gggggggggg gagtaaagac 
       61 aggaaacttt actgactaac atggcaaaac aacagaacaa caggcgaaaa tccgcaacaa 
      121 tgcgtgcagt gaagcgcatg ataaatacac acttggagca taaaaggttt gcactgatca 
      181 actcagggaa caccaatgca actgctggta cagtacaaaa tctgtccaac ggtataatcc 
      241 aaggagatga tatcaaccag agaagtggtg atcaagtgcg tatagtttca cataaacttc 
      301 acgtacgagg cactgccatc accgtcagcc agacctttag atttatctgg tttcgtgata 
      361 acatgaaccg tgggaccact cccacagttc ttgaggtgtt gaacactgcg aatttcatgt 
      421 cgcagtataa cccaatcacg ttgcagcaaa agagatttac aatactcaag gatgtaactc 
      481 tcaattgttc gctgacaggg gagagcatta aagatcggat aattaacctt ccaggacaac 
      541 tggtgaacta taatggagcg acggctgtag cagcctccaa tggtcccggc gcaatattta 
      601 tgttgcagat tggcgactcc ttggttggtc tgtgggactc ctcttatgag gctgtgtaca 
      661 cagatgcata atcccagagg ttcacaatgt tagtgatggg gcgctgaaag atgcgtagct 
      721 acccttctgg agccacttcc tggtggtaag cagaaatcca agggtacggt ggtacggtgg 
      781 aaagcagtcc cagctctgca ttgggaaccg gcttacaccc agcttagggc taaagtgtac 
      841 tacttgctca tttgtagtct aaatgagacg ttggcctcga cgtgtcgagg tggcctaagg 
      901 gattggaacc cctgatggtc gtagtcgaat ttcccgtgtt tcattccgag tctcttggtc 
      961 ataatgccat tagtaggtct agcactcaac gtaacttcaa agatatcctc cttgcaacaa 
     1021 gaatatgtgc gccgtctgtg tttaaagcgg tatattaagt gcgccggcat atcgttgttt 
     1081 ggaccagggc cccacgccgg ttggtacccg ggtggcttcc cctcgttcac agggctttag 
     1141 gagatgataa ggtatagtta ttagacaaat gcggacaaac ctgaaaagct cgctagtggt 
     1201 gggctggcca agcgaagaac ctcatccagg tatagttcta catgggaaat ttggtaccat 
     1261 ccaaacttct atgaagtcct cgactacccc aaaccccccc cccccccccc ccccccctgc 
     1321 aggtcgacct ctagagccaa gcttggctgc ctcgcgcgtt tcggtgatga cggtgaaaac 
     1381 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 
     1441 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 
     1501 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 
     1561 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 
     1621 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     1681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     1741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     1801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     1861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     1921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     1981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     2041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     2101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     2161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     2221 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 
     2281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     2341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     2401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     2461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     2521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     2581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     2641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     2701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     2761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     2821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     2881 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     2941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     3001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     3061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     3121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     3181 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     3241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     3301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     3361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     3421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     3481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     3541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 
     3601 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a 
//
