LOCUS       pUCIVHAs                4310 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3456.


COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     CDS             442..2040
                     /vntifkey="4"
                     /label=IVHAs
                     /note="gene encoding secreted haemagglutinin"
     misc_feature    442..489
                     /vntifkey="21"
                     /label=sIVHA
                     /note="signal sequence of IVHA"
     CDS             490..1476
                     /vntifkey="4"
                     /label=HA1
                     /note="HA1 chain of IVHA"
     CDS             1480..2037
                     /vntifkey="4"
                     /label=HA2f
                     /note="fragment of HA2 chain of IVHA (first 186 amino acid codons)"
     promoter        complement(2132..2215)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2310..2710)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3250..4110)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     misc_feature    2061..419
                     /vntifkey="21"
                     /label=pUC
                     /note="pUC part of the plasmid"
BASE COUNT     1210 a      1009 c      1063 g      1028 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 
      421 accgggataa ttctattaac catgaagact atcattgctt tgagctacat tttctgtctg 
      481 gttttcgccc aagaccttcc aggaaatgac aacaacagca cagcaacgct gtgcctggga 
      541 catcatgcgg tgccaaacgg aacgctagtg aaaacaatca cgaatgatca gattgaagtg 
      601 actaatgcta ctgagctggt tcagagttcc tcaacgggta aaatatgcaa caatcctcat 
      661 cgaatccttg atggaataaa ctgcacactg atagatgctc tattggggga ccctcattgt 
      721 gatggatttc aaaatgagaa atgggacctt ttcgttgaac gcagcaaagc tttcagcaac 
      781 tgttaccctt atgatgtgcc agattatgcc tcccttaggt cactagttgc ctcgtcaggc 
      841 actctggagt ttatcaatga aggcttcaat tggactgggg tcactcagaa tgggggaagc 
      901 agtgcttgca aaagaggacc tgatagcggt tttttcagta gactgaactg gttgtacaaa 
      961 tcaggaagca catatccagt gcaaaacgtg accatgccaa acaatgacaa ttctgacaaa 
     1021 ctatacattt ggggggttca ccacccgagc acggacaaag aacaaaccaa cctatatgtt 
     1081 caagcatcag ggaaagtcac agtctccacc aagagaagcc agcaaactat aatcccgaat 
     1141 gtcgggtcta gaccctgggt aaggggtctg tctagtagaa taagcatcta ttggacaata 
     1201 gttaaaccgg gagacatact ggtaattaat agtaatggga acctaattgc tcctcggggt 
     1261 tacttcaaaa tgcgcactgg gaaaagctca ataatgaggt cagatgcacc tattggcacc 
     1321 tgcagctctg aatgcatcac tccaaatgga agcattccca atgacaagcc ctttcaaaac 
     1381 gtaaacaaga tcacatatgg ggcatgtccc aagtatgtta agcaaaacac tctgaagttg 
     1441 gcaacaggga tgcggaatgt accagagaaa caaactagag gcatattcgg cgcaatagca 
     1501 ggtttcatag aaaatggttg ggagggaatg atagacggtt ggtacggttt caggcatcaa 
     1561 aattccgagg gcacaggaca agcagcagat cttaaaagca ctcaagcagc catcgaccaa 
     1621 atcaatggga aactgaatag ggtaatcgag aagacgaacg agaaattcca tcaaatcgaa 
     1681 aaggaattct cagaagtaga agggagaatt caggacctcg agaaatacgt tgaagacact 
     1741 aaaatagatc tctggtctta caatgcggag cttcttgtcg ctctggagaa ccaacataca 
     1801 attgatctga ctgactcgga aatgaacaaa ctgtttgaaa aaacaaggag gcaactgagg 
     1861 gaaaatgctg aggacatggg caatggttgc ttcaaaatat accacaaatg tgacaatgct 
     1921 tgcatagggt caatcagaaa tgggacttat gaccatgatg tatacagaga cgaagcatta 
     1981 aacaaccggt ttcagatcaa aggtgttgaa ctgaagtcag gatacaaaga ctggatctag 
     2041 ttaactaagg atccactcct gggtaccgag ctcgaattcg taatcatggt catagctgtt 
     2101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 
     2161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 
     2221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 
     2281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     2341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     2401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     2461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     2521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     2581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     2641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     2701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     2761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     2821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     2881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     2941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     3001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     3061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     3121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     3181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     3241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     3301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     3361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     3421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     3481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     3541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     3601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     3661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     3721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     3781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     3841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
     3901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     3961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     4021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     4081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     4141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     4201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 
     4261 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
//
