LOCUS       pUD1                    8905 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 4355.


COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     rep_origin      1..838
                     /vntifkey="33"
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence"
     terminator      complement(839..1109)
                     /vntifkey="43"
                     /label=CYC1T
                     /note="S. cerevisiae iso-1-cytochrome C terminator"
     CDS             complement(1198..1761)
                     /vntifkey="4"
                     /label=mDHFR
                     /note="mouse dihydrofolate reductase coding sequence"
     promoter        complement(1770..2234)
                     /vntifkey="30"
                     /label=ACTIN
                     /note="S. cerevisiae actin promoter"
     CDS             3539..4342
                     /vntifkey="4"
                     /label=URA3
                     /note="S. cerevisiae uracil 3 coding sequence"
     rep_origin      5630..6610
                     /vntifkey="33"
                     /label=2MU-O
                     /note="2ò origin, incl. region conferring stability (STB)"
     rep_origin      complement(6894..7296)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(7837..8697)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     2303 a      1950 c      1980 g      2212 t       460 others
ORIGIN
        1 ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta 
       61 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca 
      121 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg 
      181 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc 
      241 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt 
      301 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta 
      361 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca 
      421 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca 
      481 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca 
      541 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag 
      601 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa 
      661 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga 
      721 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa 
      781 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct 
      841 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt 
      901 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc 
      961 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc 
     1021 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta 
     1081 attacatgat atcgacaaag gaaaaggggg acggatctaa agccagcaaa agtcccatgg 
     1141 tcttataaaa atgcatagct ttaggagggg agcagagaac ttgaaagcat cttcctgtta 
     1201 gtctttcttc tcgtagactt caaacttata cttgatgcct ttttcctcct ggacctcaga 
     1261 gaggacgcct gggtattctg ggagaagttt atatttcccc aaatcaattt ctgggaaaaa 
     1321 cgtgtcactt tcaaattcct gcatgatcct tgtcacaaag agtctaaggt ggcctggttg 
     1381 attcatggct tcctggtaaa cagaactgcc tccgactatc caaaccatgt ctactttact 
     1441 tgccaattcc ggttgttcaa taagtcttaa ggcatcatcc aaacttttgg caagaaaatg 
     1501 agctcctcgt ggtggttctt tgagttctct actgagaact atattaattc tgtcctttaa 
     1561 aggtcgattc ttctcaggaa tggagaacca ggttttccta cccataatca ccagattctg 
     1621 tttaccttcc actgaagagg ttgtggtcat tctttggaag tacttgaact cgttcctgag 
     1681 cggaggccag ggtaggtctc cgttcttgcc aatccccata ttttgggaca cggcgacgat 
     1741 gcagttcaat ggtcgaacca tgatggcaga gcttnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2221 nnnnnnnnnn nnnnccaaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc 
     2281 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg 
     2341 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg 
     2401 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg 
     2461 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc 
     2521 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 
     2581 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 
     2641 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 
     2701 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac 
     2761 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta 
     2821 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc 
     2881 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga 
     2941 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg 
     3001 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg 
     3061 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag 
     3121 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca 
     3181 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag 
     3241 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggggggggg 
     3301 gggggggggg ggagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt 
     3361 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa 
     3421 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg 
     3481 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat 
     3541 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct 
     3601 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac 
     3661 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca 
     3721 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc 
     3781 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt 
     3841 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc 
     3901 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac 
     3961 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac 
     4021 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg 
     4081 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac 
     4141 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga 
     4201 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg 
     4261 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag 
     4321 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc 
     4381 acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga 
     4441 tttttataat gacgaaaaaa aaaaaattgg aaagaaaaag ctcccccccc cccccccccc 
     4501 cctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga 
     4561 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc 
     4621 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct 
     4681 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt 
     4741 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa 
     4801 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca 
     4861 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg 
     4921 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca 
     4981 cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa 
     5041 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca 
     5101 tctgtgaatc gcttcacgac cacgctgatg agctttaccg caggactaat gttgtgggaa 
     5161 attggagcga taagcgtgct tctgccgtgg ccaggacaac gtatactcat cagataacag 
     5221 caatacctga tcactacttc gcactagttt ctcggtacta tgcatatgat ccaatatcaa 
     5281 aggaaatgat agcattgaag gatgagacta atccaattga ggagtggcag catatagaac 
     5341 agctaaaggg tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac 
     5401 aggaggtact agactacctt tcatcctaca taaatagacg catataagta cgcatttaag 
     5461 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata 
     5521 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc 
     5581 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gaaagtatag 
     5641 gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa 
     5701 aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg 
     5761 ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac ctacccatcc 
     5821 acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat gtttatctct 
     5881 agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg aatcgaaaac 
     5941 aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag aagaaaccgt 
     6001 tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg 
     6061 cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc 
     6121 gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag 
     6181 aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat 
     6241 caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta 
     6301 gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt 
     6361 tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt 
     6421 gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc 
     6481 ttcgttggta aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat 
     6541 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag 
     6601 atgcttcgtt ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 
     6661 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 
     6721 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 
     6781 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     6841 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 
     6901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     6961 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     7021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     7081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     7141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     7201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     7261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     7321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     7381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     7441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     7501 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     7561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     7621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     7681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     7741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     7801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     7861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     7921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     7981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     8041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     8101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat 
     8161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     8221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     8281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     8341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     8401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga 
     8461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     8521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     8581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     8641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 
     8701 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     8761 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     8821 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 
     8881 cacgaggccc tttcgtcttc aagaa 
//
