LOCUS       pULG332                 6071 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3230.


COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_feature    1..590
                     /vntifkey="21"
                     /label=5FR
                     /note="5' flanking region of the lip2 gene incl. Promoter"
     CDS             591..1892
                     /vntifkey="4"
                     /label=lip2
                     /note="Moraxella strain TA144 lipase 2 gene"
     misc_feature    1893..3632
                     /vntifkey="21"
                     /label=3FR
                     /note="3' flanking region of the lip2 gene"
     misc_feature    2132..3631
                     /vntifkey="21"
                     /label=SNK
                     /note="sequence not known"
     promoter        complement(3691..3707)
                     /vntifkey="30"
                     /label=T7
                     /note="phage T7 gene 10 promoter"
     rep_origin      complement(3775..4185)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4724..5584)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     promoter        6035..6051
                     /vntifkey="30"
                     /label=SP6
                     /note="phage SP6 promoter"
     misc_feature    3633..6071
                     /vntifkey="21"
                     /label=pSP73
                     /note="pSP73 part of the plasmid"
BASE COUNT     1208 a      1067 c      1078 g      1218 t      1500 others
ORIGIN
        1 gatgtcattt tcgatggtct cattagcgtc agtatctgaa ttatttttcg gatcaaactc 
       61 tttcatggtt tcttctaaaa tactctcagc gtgctcaaca ttgtcatgtg acacgttttg 
      121 gtcatgagat tcatggttgg tgttctgctc gctcatggta gctccttagg attattaata 
      181 ataatggttg gttttaaggc acaataaact cgccaaacgc tggttattgt gcttttatta 
      241 atggcctaac tgtttttgtt cgttcttttt aaaatgcgct tcaatagctg cttgataaag 
      301 gtggtgagga taaatttcaa gcccaacctg aggaattttt tgttttagca ggataaatca 
      361 ttgcaaagcg taaggattat tactaatacc gctataaaaa gtgctcaaca ttaggataac 
      421 tggctggtag agacgattac gtttttgtca ccgatatgcg cttaacttta tcgtgactag 
      481 cttttaagat agattggaat ttaatataaa tttcacgatt aacgctatat tgacagttga 
      541 ctgataaagc cgactgactt tgttttaaca ccaatcacaa gagagggttt atgcctattc 
      601 taccagtacc ggcgctcaat gccttgctga caaaaacgat taaaaccatt aaaactgggg 
      661 ccgctaaaaa tgctcatcag catcatgttt tgcaccatac gctaaaagga ttggacaatc 
      721 tgccagcgcc ggtgctcgaa cgtatcaatc gtcgattaaa ggcgtctaca gcagagcaat 
      781 atcctctagc cgatgcccat ctgcgcctga tactcgctat tagtaataag ctcaagcgac 
      841 cgttagcaat agataagctg cccaaactac ggcagaaatt tggtactgat gcggtgtctt 
      901 tgcaagcgcc tagcgtttgg caacaaaatg cggatgcttc aggcagtaca gaaaatgccg 
      961 tcagctggca agataaaacc attgctaatg ccgatggtgg tgatatgacc gtgcgctgct 
     1021 accagaaatc gacgcaaaat agtgagagaa aaagtacgga tgaagccgcc atgctatttt 
     1081 ttcatggcgg cggattttgt atcggtgaca tcgacacgca ccatgagttt tgtcatacgg 
     1141 tctgtgctca aacgggctgg gcggtggtaa gtgtcgacta tcgcatggca cctgaatatc 
     1201 cagcgccaac tgcacttaag gactgtctgg cggcttatgc ttggctggct gaacattcac 
     1261 agtcgcttgg cgcatcacca tcgcgtattg tactatctgg tgatagtgcg ggcggttgct 
     1321 tggcagcact ggttgcacag caggttatca aacctatcga tgcactatgg caagataata 
     1381 atcaagctcc tgcagccgat aaaaaagtca atgacacttt taaaaactca ctggctgatt 
     1441 taccgcgacc tttagcacaa ctgccgctat atcctgtcac tgattatgag gctgaatatc 
     1501 caagctggga gctttatggt gaagggttgc tgctcgatca caacgatgca gaagtcttta 
     1561 attctgccta cactcagcac agcggtctac cacagtccca tccacttatc tcagtcatgc 
     1621 atggtgacaa tacgcaactg tgtcccagct atattgtcgt tgcggaatta gatatcttac 
     1681 gagatgaagg actcgcctat gccgaacttt tgcaaaaaga gggcgttcaa gtacaaactt 
     1741 atacagtgct aggagcgccg cacggtttta ttaacttaat gagtgtccat caaggtcttg 
     1801 gcaatcagac aacttatatt atcaatgagt ttgcttgtct tgtgcaaaac ctactgacca 
     1861 gtgagggtga taagccaaat ctcagagcgt gatcccaaac gaaatgggat accccaaaag 
     1921 ccgtaaccag atgacactac gaagtgactg ttattgatga cctcatagcc ataacctaag 
     1981 cggttgatgg ctttgatgat aaaattggca ggaatatttg tccattatgg gtatgccctg 
     2041 agagctgtaa atcaataggt aactcactgt gctcggtaat agcgctcggt ctgtgatcat 
     2101 gagtcgtctg aactctaccg ttgccgatat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggatcctct agagtcgacc tgcaggcatg 
     3661 caagcttcag ctgctcgagg ccggtctccc tatagtgagt cgtattaatt tcgataagcc 
     3721 aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     3781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     3841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     3901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     3961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     4021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     4081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     4141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     4201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     4261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     4321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     4381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     4441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     4501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     4561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     4621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     4681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     4741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     4801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     4861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     4921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     4981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     5041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     5101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     5161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     5221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     5281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     5341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     5401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     5461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     5521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     5581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     5641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     5701 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 
     5761 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 
     5821 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 
     5881 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 
     5941 cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa cgcggctaca 
     6001 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaaccagat 
     6061 ctgatatcat c 
//
