LOCUS       pUR250                  2705 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 862.


COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     promoter        372..455
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     misc_feature    456..493
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the E. coli lacZ gene, incl. RBS"
     CDS             494..701
                     /vntifkey="4"
                     /label=lacZ
                     /note="æ part of the E. coli lacZ gene"
     rep_origin      complement(702..1104)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(1645..2505)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      677 a       697 c       669 g       656 t         6 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatatac 
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 
      241 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gcccaatacg caaaccgcct 
      301 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 
      361 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 
      421 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 
      481 acaggaaaca gctatgacca tgattacgcc aagctttcta gagtcgacgg atccggggaa 
      541 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 
      601 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 
      661 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctcttccg cttcctcgct 
      721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
      781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
      841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
      901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
      961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     1021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     1081 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     1141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     1201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     1261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     1321 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     1381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     1441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     1501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     1561 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     1621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     1681 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     1741 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     1801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     1861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     1921 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     1981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     2041 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     2101 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     2161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     2221 atagtgtatg cggcgaccga gttgctcttg cccggcnnnn nnacgggata ataccgcgcc 
     2281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     2341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     2401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     2461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     2521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     2581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     2641 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     2701 tcgtc 
//
