LOCUS       YDp-H                   3865 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3353.


COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     CDS             721..1380
                     /vntifkey="4"
                     /label=HIS3
                     /note="S. cerevisiae histidine 3 coding sequence"
     promoter        complement(1687..1770)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(1862..2264)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2805..3665)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     misc_feature    432..1581
                     /vntifkey="21"
                     /label=Sce
                     /note="S. cerevisiae histidine 3 insert"
     misc_feature    1582..431
                     /vntifkey="21"
                     /label=pUC
                     /note="pUC part of the plasmid"
BASE COUNT      992 a       922 c       943 g      1008 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cccggggatc cggtgattga 
      421 ttgagcaagc ttaggagtca ctgccaggta tcgtttgaac acggcattag tcagggaagt 
      481 cataacacag tcctttcccg caattttctt tttctattac tcttggcctc ctctagtaca 
      541 ctctatattt ttttatgcct cggtaatgat tttcattttt ttttttccac ctagcggatg 
      601 actctttttt tttcttagcg attggcatta tcacataatg aattatacat tatataaagt 
      661 aatgtgattt cttcgaagaa tatactaaaa aatgagcagg caagataaac gaaggcaaag 
      721 atgacagagc agaaagccct agtaaagcgt attacaaatg aaaccaagat tcagattgcg 
      781 atctctttaa agggtggtcc cctagcgata gagcactcga tcttcccaga aaaagaggca 
      841 gaagcagtag cagaacaggc cacacaatcg caagtgatta acgtccacac aggtataggg 
      901 tttctggacc atatgataca tgctctggcc aagcattccg gctggtcgct aatcgttgag 
      961 tgcattggtg acttacacat agacgaccat cacaccactg aagactgcgg gattgctctc 
     1021 ggtcaagctt ttaaagaggc cctactggcg cgtggagtaa aaaggtttgg atcaggattt 
     1081 gcgcctttgg atgaggcact ttccagagcg gtggtagatc tttcgaacag gccgtacgca 
     1141 gttgtcgaac ttggtttgca aagggagaaa gtaggagatc tctcttgcga gatgatcccg 
     1201 cattttcttg aaagctttgc agaggctagc agaattaccc tccacgttga ttgtctgcga 
     1261 ggcaagaatg atcatcaccg tagtgagagt gcgttcaagg ctcttgcggt tgccataaga 
     1321 gaagccacct cgcccaatgg taccaacgat gttccctcca ccaaaggtgt tcttatgtag 
     1381 tgacaccgat tatttaaagc tgcagcatac gatatatata catgtgtata tatgtatacc 
     1441 tatgaatgtc agtaagtatg tatacgaaca gtatgatact gaagatgaca aggtaatgca 
     1501 tcattctata cgtgtcattc tgaacgaggc gcgctttcct tttttctttt tgctttttct 
     1561 ttttttttct cttgaactcg aagcttgctc aatcaatcac cggatccgtc gacctgcagc 
     1621 caagctagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     1681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     1741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     1801 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     1861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     1921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     1981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     2041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     2101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     2161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     2221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     2281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     2341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     2401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     2461 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     2521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     2581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     2641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     2701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     2761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     2821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     2881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     2941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     3001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     3061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     3121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     3181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     3241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     3301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     3361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     3421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     3481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     3541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     3601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     3661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     3721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     3781 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
     3841 aggcgtatca cgaggccctt tcgtc 
//
