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BCCM/LMBP DNA library details

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Library name: Pichia pastoris library   Add to cart
Library type: cDNA
Library accession number: LMBP LIB16
Organism: Pichia pastoris
Priming method: NotI-(dT)15 (unidirectional)
Vector name: pSCGAL10-SN
Vector sequence file: p2471.txt
Vector circular map: p2471.pdf
Vector accession number: LMBP 2471
Vector type: yeast expression vector
Cloning sites: SfiI-NotI
Library coverage: 3,0 x 10E5
Average insert size: 1,19 kb
Distributed as: pure DNA
DNA concentration: 1 µg/µl
Volume: 5 µl
Further information: Pichia pastoris GTS115 was grown in minimal SDC medium, consisting of 0.67% bacto-yeast nitrogen base without amino acids, 2% dextrose and 0.2% cas amino acids.

The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The resulting host/plasmid combination can then be streaked on selective solid medium (LB + 100 µg/ml ampicillin) and cultivated at 37°C.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: /
Related reference: /
Restricted distribution: - BCCM MTA

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