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BCCM/GeneCorner DNA library details

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Library name: Candida albicans library  
Library type: cDNA
Library accession number: LMBP LIB18
Organism: Candida albicans
Priming method: NotI-(dT)15 (unidirectional)
Vector name: pGAL1PNiST-1
Vector sequence file: p4099.txt
Vector circular map: p4099.pdf
Vector accession number: LMBP 4099
Vector type: Candida albicans expression vector
Cloning sites: SfiI-NotI antisense
Library coverage: 2,0 x 10E6
Average insert size: 1,50 kb
Distributed as: pooled library DNA
DNA concentration: 1,8 µg/µl
Volume: 5 µl
Further information: Candida albicans was grown in minimal SD medium, consisting of 0.67% bacto-yeast nitrogen base without amino acids and 2% dextrose.
The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The gDNA library was deposited by Janssen Pharmaceutica N.V.1.
1 Beerse, Belgium
Library reference: De Backer et al., Nat. Biotechnol. 19 (2001), 235-241 [PMID: 11231556]
Related reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.

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