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BCCM/GeneCorner DNA library details

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Library name: Candida albicans library  
Library type: gDNA
Library accession number: LMBP LIB21
Organism: Candida albicans
Vector name: YCp50
Vector sequence file: ycp50.txt
Vector circular map: ycp50.pdf
Vector accession number: ATCC 37419
Vector type: yeast expression vector
Cloning sites: BamHI
Library coverage: 1,5 x 10E5
Average insert size: 10-23 kb
Distributed as: pooled library DNA
DNA concentration: 800 ng/µl
Volume: 5 µl
Further information: YCp50 was BamHI cut and the genomic Candida albicans Sau3AI fragments were ligated.
The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The gDNA library was deposited by Janssen Pharmaceutica N.V.1.
1 Beerse, Belgium
Library reference: De Backer et al., Nat. Biotechnol. 19 (2001), 235-241 [PMID: 11231556]
Related reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.

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