|Library name:||Human TRC1 library|
|Library accession number:||LMBP LIB28|
|Available clones:||See Sigma Aldrich for the available human TRC1 clones|
|Vector sequence file:||p7210.txt|
|Vector circular map:||p7210.pdf|
|Vector accession number:||LMBP 7210|
|Vector type:||lentiviral vector|
|Average insert size:||57 bp|
|Distributed as:||individual clones as active cultures or plasmid DNA|
|Further information:||The host strain of the individual clones is Escherichia coli K12 DH5αT1R.
The individual clones can be grown in selective medium (TB-8gly + 100 µg/ml ampicillin) and cultivated at 37°C.
pLKO.1-puro-shRNA (LMBP 7210) can be considered as a representative for all TRCN plasmids available at BCCM/GeneCorner.
Check https://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna/individual-genes.html for the available mouse TRC1.5 clones or contact BCCM/GeneCorner.
|History of deposit:||The library clones were purchased from Sigma-Aldrich in the frame of the Hercules project "RNAi collection and associated facilities".
|Related reference:||Stewart et al., RNA 9 (2003), 493-501 [PMID: 12649500]
Moffat et al., Cell 124 (2006), 1283-1298 [PMID: 16564017]
|Restricted distribution:||- Restricted to project-related users
- The buyer cannot sell or transfer this product, its components nor materials made using this product or its components to another research group.
- Visit www.sigma.com/shRNA for more, up-to-date info on the limited use of this product
- Acknowledge BCCM/GeneCorner (supplier) and the Hercules Foundation (funder) in any publication resulting from the use of a shRNA clone