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BCCM/GeneCorner DNA library details

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Library name: Human TRC1.5 library  
Library type: shRNA
Library accession number: LMBP LIB30
Organism: Homo sapiens
Available clones: See Sigma Aldrich for the available mouse TRC1.5 clones
Vector name: pLKO.1-puro-shRNA
Vector sequence file: p7210.txt
Vector circular map: p7210.pdf
Vector accession number: LMBP 7210
Vector type: lentiviral vector
Cloning sites: AgeI-EcoRI
Library coverage: 17567
Average insert size: 57 bp
Distributed as: individual clones as active cultures or plasmid DNA
Further information: The host strain of the individual clones is Escherichia coli K12 DH5αT1R.
The individual clones can be grown in selective medium (TB-8gly + 100 µg/ml ampicillin) and cultivated at 37°C.
pLKO.1-puro-shRNA (LMBP 7210) can be considered as a representative for all TRCN plasmids available at BCCM/GeneCorner.
Check https://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna/individual-genes.html for the available mouse TRC1.5 clones or contact BCCM/GeneCorner.
History of deposit: The library clones were purchased from Sigma-Aldrich in the frame of the Hercules project "RNAi collection and associated facilities".
Schnelldorf, Germany
Library reference: - [PMID: -]
Related reference: Stewart et al., RNA 9 (2003), 493-501 [PMID: 12649500]
Moffat et al., Cell 124 (2006), 1283-1298 [PMID: 16564017]
Restricted distribution: - Restricted to project-related users
- The buyer cannot sell or transfer this product, its components nor materials made using this product or its components to another research group.
- Visit www.sigma.com/shRNA for more, up-to-date info on the limited use of this product
- Acknowledge BCCM/GeneCorner (supplier) and the Hercules Foundation (funder) in any publication resulting from the use of a shRNA clone

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