|Library name:||Human ORFeome|
|Library accession number:||LMBP LIB32|
|Available clones:||ORFeome entry clones|
|Vector sequence file:||p9210.txt|
|Vector circular map:||p9210.pdf|
|Vector accession number:||LMBP 9210|
|Vector type:||Gateway donor vector|
|Cloning sites:||Gateway BP reaction|
|Average insert size:||1126 bp|
|Distributed as:||individual clones as active cultures or plasmid DNA|
|Further information:||The host strain of the individual clones is Escherichia coli K12 DH5α
The individual clones can be grown in selective medium (LB-Lennox + 50 µg/ml spectinomycin) and cultivated at 37°C.
pENTR223-ORF (LMBP 9210) can be considered as a representative for all ORF plasmids available at BCCM/GeneCorner.
Related data can be found on: http://horfdb.dfci.harvard.edu/index.php?page=orfsearch
Sequences of the different ORFs can be found on: http://horfdb.dfci.harvard.edu/index.php?page=home
|History of deposit:||This plasmid was deposited by Dr M. Vidal1 2 3 and Dr D. Hill1 2 3. It was received from Dr I. Lemmens4.
1 Center for Cancer Systems Biology (CCSB), Boston, Massachusetts, USA
2 Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA
3 Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
4 Department of Medical Protein Research, VIB, Ghent, Belgium
|Library reference:||Yang et al., Nat. Methods 8 (2011), 659-661 [PMID: 21706014]|
|Related reference:||Rual et al., Genome Res. 14 (2004), 2128-2135 [PMID: 15489335]|
|Restricted distribution:||- BCCM MTA adapted for hORFeome clones
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
- RECIPIENT agrees to refer to 'Yang et al., Nat. Methods 8 (2011), 659-661 [PMID: 21706014]' in the first publication making use of the MATERIAL.