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BCCM/LMBP DNA library details

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Library name: Human ORFeome  
Library type: cDNA
Library accession number: LMBP LIB32
Organism: Homo sapiens
Available clones: ORFeome entry clones
Vector name: pDONR223
Vector sequence file: pdonr223.txt
Vector circular map: pdonr223.pdf
Vector accession number: Invitrogen
Vector type: Gateway donor vector
Cloning sites: Gateway BP reaction
Library coverage: 12680
Average insert size: 1126 bp
Distributed as: individual clones
Further information: The host strain of the individual clones in the 96-well plates is Escherichia coli K12 DH5α.

Related data can be found on: http://horfdb.dfci.harvard.edu/index.php?page=orfsearch
Sequences of the different ORFs can be found on: http://horfdb.dfci.harvard.edu/index.php?page=home
History of deposit: This plasmid was deposited by Dr M. Vidal1 2 3 and Dr D. Hill1 2 3. It was received from Dr I. Lemmens4.
1 Center for Cancer Systems Biology (CCSB), Boston, Massachusetts, USA
2 Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA
3 Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
4 Department of Medical Protein Research, VIB, Ghent, Belgium
Library reference: Yang et al., Nature Methods 8 (2011), 659-661 [PMID: 21706014]
Related reference: Rual et al., Genome Res. 14 (2004), 2128-2135 [PMID: 15489335]
Restricted distribution: - BCCM MTA adapted by depositor
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
- RECIPIENT agrees to refer to 'Yang et al., Nat. Methods 8 (2011), 659-661

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