|Library name:||Aspergillus niger library|
|Library accession number:||LMBP LIB8|
|Priming method:||NotI-(dT)15 (unidirectional)|
|Vector sequence file:||p2471.txt|
|Vector circular map:||p2471.pdf|
|Vector accession number:||LMBP 2471|
|Vector type:||yeast expression vector|
|Library coverage:||4,0 x 10E5|
|Average insert size:||1,25 kb|
|Distributed as:||pooled library DNA|
|DNA concentration:||200 ng/µl|
|Further information:||Aspergillus niger N402 was grown in rich YPD medium, consisting of 1% bacto-yeast extract, 2% bacto-peptone and 2% dextrose.
The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
|History of deposit:||The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
|Library reference:||Veldhuisen et al., Mol. Gen. Genet. 256 (1997), 446-455 [PMID: 9393442]|
|Restricted distribution:||- BCCM MTA|