Last data update: 24 January 2024 16:39 CET
Plasmid name: R57b (LMBP 7976)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) Fuschin acid (fa) Gentamicin (Gm) Methyl-β-D-galactopyranoside (Tra+) Neomycin (neo; kanamycin (kan)) Sulfonamide (Sul) |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | R57b is a natural plasmid. This is a reference strain for the IncC incompatibility family. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Natural plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture |
| Host for distribution: | Escherichia coli K12 J53 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin and kanamycin. |
| Other culture collection numbers: | SCK CM0117 |
Refer in your Materials and Methods: |
R57b (LMBP 7976) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.