Last data update: 24 January 2024 16:39 CET
Plasmid name: pACYC177 (LMBP 1346)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p1346.gb
(View with Genome Compiler) p1346.txt p1346.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) Kanamycin (kan) |
Replicon: | Escherichia coli plasmid p15A origin |
Copy number: | Low copy number |
Host range: | Escherichia coli |
Parental clone: | p15A; Tn903; Tn3 |
Further information: | This plasmid is compatible with ColE1- or pMB1-derived plasmids. In the presence of chloramphenicol or spectinomycin, the amplification of the plasmid is very limited (2x). The kanamycin resistance gene is excisable as a HaeII fragment. The nucleotide sequence of the vector corresponds with the EMBL Nucleotide Sequence Database accession number X06402.1, except for 2 nucleotides and a change in starting position. |
EMBL Accession number: | X06402.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 09/01/1990 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AlwNI/HindIII and BamHI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr S. Cohen(1). (1) Stanford University School of Medicine, USA |
Plasmid reference: | Chang et al., J. Bacteriol. 134 (1978), 1141-1156 [PMID: 149110] |
Related plasmid reference: | Mok et al., Nucleic Acids Res. 19 (1991), 2321-2323 [PMID: 2041772] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | DSM 5587 NCCB 3009 PC-V 3009 |
Refer in your Materials and Methods: |
pACYC177 (LMBP 1346) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Cohen and was published in Chang et al., 1978. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.