Last data update:
19 February 2019 04:10 CET
Name: | pBR322 Add to cart |
Accession number: | LMBP 140 |
Sequence file: | LMBP sequence: p140.gb, p140.txt View with Genome Compiler |
Circular map: | p140.pdf |
Status: | GeneCorner core plasmid |
Cloned DNA: | - |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) Tetracycline (tet) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Further information: | HindIII does not always inactivate the tetracycline resistance gene. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number J01749 .1. |
EMBL Accession number: | J01749.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 23/01/1989 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, HindII, HindIII/PstI and RsaI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr F. Bolivar(1). (1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico |
Plasmid reference: | Bolivar et al., Gene 2 (1977), 95-113 [PMID: 344137] |
Related plasmid reference: | Sutcliffe, Cold Spring Harb. Symp. Quant. Biol. 43 (1979), 77-90 [PMID: 383387] Peden, Gene 22 (1983), 277-280 [PMID: 6307828] Lathe et al., DNA 3 (1984), 173-182 [PMID: 6327214] Nugent et al., J. Gen. Microbiol. 132 (1986), 1021-1026 [PMID: 2428924] Watson, Gene 70 (1988), 399-403 [PMID: 3063608] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 JMB9 |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | DSM 3879 NCCB 3019 PC-V 3019 |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.