Last data update: 24 January 2024 16:39 CET
Plasmid name: pC2HB-GC (LMBP 9242)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9242.gb |
| Sequence analysis results Genecorner: |
NGS: c02-gc-may2021.fasta |
| Cloned DNA: |
Candida albicans gDNA B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) SV40 large T-antigen nuclear localization signal (NLS); N-terminal Staphylococcus aureus LexA repressor cDNA (LexA, lexA DNA-binding domain, lexA DBD, lexAbd, GeneID 3920198); N-terminal Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
| Promoter: | Candida albicans sulfate adenylyltransferase promoter (MET3) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter Escherichia coli β-lactamase promoter (amp) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans actin terminator (ACT1) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) Candida maltosa LEU2 (CmLEU2); auxotrophic Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Candida albicans; leu2(-), integrative |
| Parental clone: | pC2HB |
| Further information: | The plasmid was created by cloning a Gateway cassette into the pC2HB vector. This Gateway destination vector is meant as a bait plasmid for yeast two-hybrid analysis, in which the gene of interest can be cloned via Gateway recombination. The plasmid can be linearised by NotI restriction to allow genomic integration. This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner. The nucleotide sequence of the S. aureus lexA cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AY033082.1. Other name of the plasmid is pC2HB-gc. |
| EMBL Accession number: | AY033082.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 18/06/2014 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Van Dijck(1) (2). It was constructed by Dr H. Tournu(1) (2) and Dr B. Stynen(1) (2). (1) Department of Molecular Biology, VIB, Leuven, Belgium (2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Schoeters et al., mSphere 3 (2018), e00391-18 [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pC2HB-GC (LMBP 9242) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Van Dijck . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.