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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pCfB132 (LMBP 9506)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p9506.gb (View with Genome Compiler)
p9506.txt
p9506.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
Saccharomyces cerevisiae URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB)
Host range: Escherichia coli
Saccharomyces cerevisiae; ura3(-)
Parental clone: pESC-URA; pCfB49
Further information: The plasmid was constructed by PCR amplifying the uracil-specific excision reaction (USER) cassette, ccdB gene and chloramphenicol resistance gene from pCfB49 and ligating the fragment into pESC-URA. Finally, the ccdB gene and chloramphenicol resistance gene were removed to obtain pCfB132.
The plasmid contains a S. cerevisiae 2μ FLP Recombinase Target site (FRT site), next to the USER cloning cassette (AsiSI and Nb.BsmI).
The plasmid is an episomal replication vector intended for USER cloning and USER fusion.
The nucleotide sequence of the parental pESC-URA vector corresponds with the EMBL Nucleotide Sequence Database accession number AF063585.2.
Other name of the plasmid is pESC-URA-USER.
EMBL Accession number: AF063585.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 04/12/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HincII, PvuI, SspI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr I. Borodina(1). It was constructed by Dr. N.B. Jensen(1).
(1) The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark
Plasmid reference: Jensen et al., FEMS Yeast Res. 14 (2014), 238-248 [PMID: 24151867] [DOI: 10.1111/1567-1364.12118]
Related plasmid reference: Nour-Eldin et al., Nucleic Acids Res. 34 (2006), e122 [PMID: 17000637]
Geu-Flores et al., Nucleic Acids Res. 35 (2007), e55 [PMID: 17389646]
Restricted distribution: - Depositor's Material Transfer Agreement (DTU-BCCM/MTA)
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCfB132 (LMBP 9506) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Borodina and was published in Jensen et al., 2014.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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