Last data update: 24 January 2024 16:39 CET
Plasmid name: pCfB1976 (LMBP 9510)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9510.gb |
| Sequence analysis results Genecorner: |
NGS: h04-gc-dec2019.fasta |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP) |
| Promoter: | Escherichia coli lac operon promoter Escherichia coli DAHP synthase promoter |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli Rho-independant termination signal |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC19 |
| Further information: | The plasmid was constructed by PCR amplifying the GFP expression cassette and cloning it into the SmaI-opened pUC19 vector. The GFP CDS is controlled by a constitutive bacterial promoter and a strong bacterial termination sequence. Other name of the plasmid is pUC19-GFP-cassette. |
| EMBL Accession number: | - |
| Latest sequence update: | 04/12/2014 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/KpnI, HaeII, PvuII and ScaI. This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr I. Borodina(1). It was constructed by Prof. Dr I. Borodina(1) and Dr H. Luo(1). (1) The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark |
| Plasmid reference: | - |
| Related plasmid reference: | Mikkelsen et al., Metab. Eng. 14 (2012), 104-111 [PMID: 22326477] [DOI: 10.1016/j.ymben.2012.01.006] Nour-Eldin et al., Nucleic Acids Res. 34 (2006), e122 [PMID: 17000637] Jensen et al., FEMS Yeast Res. 14 (2014), 238-248 [PMID: 24151867] [DOI: 10.1111/1567-1364.12118] |
| Restricted distribution: | - Depositor's Material Transfer Agreement (DTU-BCCM/MTA) |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCfB1976 (LMBP 9510) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Borodina. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.