Last data update: 24 January 2024 16:39 CET
Plasmid name: pCfB220 (LMBP 9508)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9508.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae LEU2; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
Parental clone: | pESC-LEU; pCfB132 |
Further information: | The plasmid was constructed by excising the 26 bp fragment carrying the uracil-specific excision reaction (USER) cassette from pCfB132 and ligating it as a SacI/XhoI fragment into the SacI/XhoI opened pESC-LEU vector. The plasmid contains a S. cerevisiae 2μ FLP Recombinase Target site (FRT site), next to the USER cloning cassette (AsiSI and Nb.BsmI). The plasmid is an episomal replication vector intended for USER cloning and USER fusion. Other name of the plasmid is pESC-LEU-USER. |
EMBL Accession number: | - |
Latest sequence update: | 04/12/2014 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: KpnI, PvuI, ScaI and SspI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr I. Borodina(1). It was constructed by Dr. N.B. Jensen(1). (1) The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark |
Plasmid reference: | Jensen et al., FEMS Yeast Res. 14 (2014), 238-248 [PMID: 24151867] [DOI: 10.1111/1567-1364.12118] |
Related plasmid reference: | Nour-Eldin et al., Nucleic Acids Res. 34 (2006), e122 [PMID: 17000637] Geu-Flores et al., Nucleic Acids Res. 35 (2007), e55 [PMID: 17389646] |
Restricted distribution: | - Depositor's Material Transfer Agreement (DTU-BCCM/MTA) |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCfB220 (LMBP 9508) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Borodina and was published in Jensen et al., 2014. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.