GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pCfB391 (LMBP 9505)

Add to cart

New search Print data sheet
Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p9505.gb (View with Genome Compiler)
p9505.txt
p9505.pdf
Sequence
analysis results
Genecorner:

NGS: e11-gc-dec2018.fasta

Cloned DNA: -
Promoter: Phage T7 gene 10 promoter (T7g10)
Eremothecium gossypii translation elongation factor 1α promoter (TEF1α)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Eremothecium gossypii translation elongation factor 1α terminator (TEF1α)
Selection marker: Ampicillin (amp)
Schizosaccharomyces pombe HIS5; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Saccharomyces cerevisiae; his5(-), integrative
Parental clone: pCfB387
Further information: The plasmid was constructed based on pCfB387 by replacing the direct repeats flanked K. lactis URA3 selection marker with a LoxP-flanked S. pombe HIS5 marker. The selection marker exchange was accomplished by Uracil-Specific Excision Reaction (USER).
The plasmid contains two S. cerevisiae 2μ FLP Recombinase Target sites (FRT sites), two S. cerevisiae chromosome XI integration targeting sequences and two bacteriophage P1 Cre recombinase target sites (loxP sites), next to the USER cloning cassette (AsiSI and Nb.BsmI).
The plasmid is part of the EasyClone integrative vector set and contains a loxP-flanked auxotrophic selection marker. This set allows simultaneous expression of multiple genes in S. cerevisiae via genomic integration, with an option of recycling selection markers.
This plasmid allows for integration of a selection marker on S. cerevisiae chromosome XI, in between two essential genetic elements. The integration site provides a good level of gene expression, has a minimum risk of spontaneous loop out, and does not impair growth.
Other name of the plasmid is pXI-5-LoxP-SpHIS5.
EMBL Accession number: -
Latest sequence update: 04/12/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII/XhoI, HaeII, NsiI and SpeI.

This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr I. Borodina(1). It was constructed by Dr. N.B. Jensen(1).
(1) The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark
Plasmid reference: Jensen et al., FEMS Yeast Res. 14 (2014), 238-248 [PMID: 24151867] [DOI: 10.1111/1567-1364.12118]
Related plasmid reference: Mikkelsen et al., Metab. Eng. 14 (2012), 104-111 [PMID: 22326477] [DOI: 10.1016/j.ymben.2012.01.006]
Restricted distribution: - Depositor's Material Transfer Agreement (DTU-BCCM/MTA)
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCfB391 (LMBP 9505) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Borodina and was published in Jensen et al., 2014.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search