Last data update: 24 January 2024 16:39 CET
Plasmid name: pETM54 (LMBP 13756)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli thiol:disulfide oxidoreductase DsbA cDNA (dsbA, dsf, iarA, ppfA, GeneID 948353); N-terminal Histidine tag (His-tag); N-terminal Human rhinovirus 3C protease cleavage site (PreScission site, PRS); N-terminal Histidine tag (His-tag); C-terminal Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Further information: | The plasmid is used for recombinant protein production in E. coli. |
| EMBL Accession number: | - |
| Latest sequence update: | 17/02/2023 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr Kim Remans(1). It was constructed by Dr A. Geerlof(1). (1) Protein Expression and Purification Core Facility, EMBL Heidelberg, Germany |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM/MTA adapted by EMBL - Restricted to academic users - Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pETM54 (LMBP 13756) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Kim Remans. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.