Last data update:
20 March 2019 04:17 CET
|Name:||pEX1SODOFF Add to cart|
|Accession number:||LMBP 5022|
|Sequence file:||Depositor's sequence: p5022.gb View with Genome Compiler|
|Status:||GeneCorner core plasmid|
|Cloned DNA:||Nicotiana plumbaginifolia manganese superoxide dismutase cDNA (MnSOD)|
|Promoter:||Agrobacterium tumefaciens Ti-plasmid nopaline synthase promoter (nos)
Cauliflower mosaic virus (CaMV) 35S promoter
Escherichia coli lac operon promoter; mutant (lacUV5)
|Terminator:||Agrobacterium tumefaciens Ti-plasmid octopine synthase terminator (ocs)|
|Selection marker:||Neomycin (neo; kanamycin (kan))
Streptomycin (Sm; spectinomycin (Sp))
|Replicon:||Escherichia coli plasmid pMB1 origin
Pseudomonas plasmid pVS1 origin
|Host range:||Escherichia coli
|Parental clone:||pSOD1; pGSJ780A|
|Further information:||The plasmid was constructed by inserting the 956 bp HpaI-SmaI fragment of pSOD1, containing the Nicotiana plumbaginifolia manganese superoxide dismutase (MnSOD) cDNA, into the ClaI (filled-in) opened pGSJ780A vector. The orientation of the inserted fragment is antisense relative to the CaMV promoter.
The plasmid contains a neomycin resistance cassette consisting of the Agrobacterium tumefaciens Ti-plasmid nopaline synthase (nos) promoter, the neomycin (neo, nptI) resistance gene, and the A. tumefaciens Ti-plasmid octopine synthase (ocs) terminator, that can be used for selection of transformed plants.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of the MnSOD cDNA corresponds with the Genbank accession number X14482.1.
|EMBL Accession number:||X14482.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||09/09/2005|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI/ClaI, BglII, EcoRV/NheI, HindII, HindIII/SacII, HpaI/SmaI, NdeI and PvuII.
The PvuII site at position 12718 could not be experimentally confirmed. The compiled nucleotide sequence has not been adapted accordingly.
|History of deposit:||This plasmid was deposited by Prof. Dr D. Inzδ(1) (2) and constructed by C. Bowler(1) (2).
(1) Department of Plant Systems Biology, VIB, Ghent, Belgium
(2) Department of Plant Systems Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + spectinomycin (150 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.