Last data update: 24 January 2024 16:39 CET
Plasmid name: pGSCZSOD31 (LMBP 5019)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5019.gb
(View with Genome Compiler) p5019.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli β-glucuronidase gene (gus) |
| Promoter: | Nicotiana plumbaginifolia copper/zinc superoxide dismutase promoter (CuZnSOD) Agrobacterium tumefaciens Ti-plasmid nopaline synthase promoter (nos) Escherichia coli lac operon promoter; mutant (lacUV5) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Agrobacterium tumefaciens Ti-plasmid octopine synthase terminator (ocs) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) Streptomycin (Sm; spectinomycin (Sp)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Pseudomonas plasmid pVS1 origin |
| Host range: | Escherichia coli Plant cells |
| Parental clone: | pGSV4; pGUSSOD31 |
| Further information: | The construction strategy of the plasmid is described in Hδrouart et al. (1993). The plasmid contains an approx. 2500 bp genomic fragment of Nicotiana plumbaginifolia (tobacco), containing the upstream sequence of the cytosolic copper/zinc superoxide dismutase coding sequence (CuZnSOD), placed upstream of the Escherichia coli β-glucuronidase reporter gene (gus). A neomycin resistance cassette consisting of the Agrobacterium tumefaciens Ti-plasmid nopaline synthase (nos) promoter, the neomycin (neo, nptII) resistance gene, and the A. tumefaciens Ti-plasmid octopine synthase (ocs) terminator, can be used for selection of transformed plants. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of the CuZnSOD promoter corresponds with the Genbank accession number L08253.1. |
| EMBL Accession number: | L08253.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/06/2005 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, NcoI, SpeI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr D. Inzé(1) (2) and constructed by D. Herouart(1) (2). (1) Department of Plant Systems Biology, VIB, Ghent, Belgium (2) Department of Plant Systems Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Hérouart et al., Proc. Natl. Acad. Sci. U.S.A. 90 (1993), 3108-3112 [PMID: 8464930] |
| Related plasmid reference: | Peleman et al., Plant Cell 1 (1989), 81-93 [PMID: 2535470] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + spectinomycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGSCZSOD31 (LMBP 5019) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr D. Inzé and constructed by D. Herouart and was published in Hérouart et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.