GeneCorner plasmid details

Last data update: 17 June 2019 04:16 CEST  

Name: pGSCZSOD32   Add to cart
Accession number: LMBP 5020
Sequence file: Depositor's sequence:     View with Genome Compiler
Circular map: p5020.pdf
Status: GeneCorner core plasmid
Cloned DNA: Escherichia coli β-glucuronidase gene (gus)
Promoter: Nicotiana plumbaginifolia copper/zinc superoxide dismutase promoter (CuZnSOD); intronless
Agrobacterium tumefaciens Ti-plasmid nopaline synthase promoter (nos)
Escherichia coli lac operon promoter; mutant (lacUV5)
Escherichia coli lac operon promoter
binding site:
Terminator: Agrobacterium tumefaciens Ti-plasmid octopine synthase terminator (ocs)
Selection marker: Neomycin (neo; kanamycin (kan))
Streptomycin (Sm; spectinomycin (Sp))
Replicon: Escherichia coli plasmid pMB1 origin
Pseudomonas plasmid pVS1 origin
Host range: Escherichia coli
Plant cells
Parental clone: pGSV4; pGUSSOD32
Further information: The construction strategy of the plasmid is described in Hδrouart et al. (1993).
The plasmid contains an approx. 1000bp genomic fragment of Nicotiana plumbaginifolia (tobacco), containing the intronless upstream sequence of the cytosolic copper/zinc superoxide dismutase coding sequence (CuZnSOD), placed upstream of the Escherichia coli β-glucuronidase reporter gene (gus).
A neomycin resistance cassette consisting of the Agrobacterium tumefaciens Ti-plasmid nopaline synthase (nos) promoter, the neomycin (neo, nptII) resistance gene, and the A. tumefaciens Ti-plasmid octopine synthase (ocs) terminator, can be used for selection of transformed plants.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727071.1.
The nucleotide sequence of the CuZnSOD promoter corresponds with the Genbank accession number L08253.1
EMBL Accession number: L08253.1, view at EMBL, GenBank, DDBJ
LT727071.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 01/07/2005
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, NcoI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr D. Inzδ(1) (2) and constructed by D. Herouart(1) (2).
(1) Department of Plant Systems Biology, VIB, Ghent, Belgium
(2) Department of Plant Systems Biology, Ghent University, Ghent, Belgium
Plasmid reference: Hérouart et al., Proc. Natl. Acad. Sci. U.S.A. 90 (1993), 3108-3112 [PMID: 8464930]
Related plasmid reference: Peleman et al., Plant Cell 1 (1989), 81-93 [PMID: 2535470]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + spectinomycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.


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