Last data update: 24 January 2024 16:39 CET
Plasmid name: pLenti6-strep-hMLKL-S358D-FLAG (LMBP 8802)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p8802.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human mixed lineage kinase domain like pseudokinase cDNA (MLKL, GeneID 197259); mutated sequence Strep-tag II; N-terminal FLAG epitope tag; C-terminal |
Promoter: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 5' LTR) Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter Simian virus 40 early promoter (SV40 early) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 3' LTR); modified HIV-1 3' LTR with deleted U3 region (ΔU3/3' LTR) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) Puromycin (puro) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pLenti6-Flag puro; pENTR3C-strep-hMLKL-S358D |
Further information: | This Lentiviral Gateway Expression vector was constructed by cloning the strep-tagged human MLKL coding sequence, containing an S358D mutation, into the pLenti6-Flag puro vector via Gateway recombination with pENTR3C-strep-hMLKL-S358D. The U3 region of the HIV-1 3' LTR is deleted (ΔU3 or dU3) and facilitates self-inactivation of the HIV-1 5' LTR after transduction to enhance the biosafety of the vector. The element also contains a polyadenylation signal for transcription termination and polyadenylation of mRNA in transduced cells. The vector contains the psi packaging signal, allowing viral packaging, and the rev response element (RRE) from HIV-1, permitting Rev-dependent nuclear export of unspliced viral mRNA. Because of the high risk for recombination, plasmid integrity should be checked via PvuII restriction digest after each subcultivation. The nucleotide sequence of the wild type human MLKL coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AK091708.1. The nucleotide sequence of the pLenti6-Flag puro vector corresponds with the EMBL Nucleotide Sequence Database accession number LT009452.1. Other name of the plasmid is pLenti6(puro)-FLAG-dest-strep-hMLKL(FL)-S358D-stop. |
EMBL Accession number: | AK091708.1, view at EMBL, GenBank, DDBJ LT009452.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 05/10/2016 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr Y. Dondelinger(1) (2) and Prof. Dr M. Bertrand(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | De Groote et al., BioTechniques 60 (2016), 252-259 [PMID: 27177818] [DOI: 10.2144/000114417] Dondelinger et al., Cell Rep. 7 (2014), 971-981 [PMID: 24813885] [DOI: 10.1016/j.celrep.2014.04.026] |
Restricted distribution: | - BCCM MTA |
Distributed as: | plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 in E. coli; L2 in mammalian cells |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLenti6-strep-hMLKL-S358D-FLAG (LMBP 8802) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Y. Dondelinger and Prof. Dr M. Bertrand . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.