Last data update: 24 January 2024 16:39 CET
Plasmid name: pOSCAR (LMBP 8055)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8055.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam) Streptomycin (Sm; spectinomycin (Sp)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Pseudomonas plasmid pVS1 origin |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Ascomycete fungi |
| Parental clone: | pPZP-RCS2; pTOPO-attP2R-ccdB-attP3 |
| Further information: | This Gateway donor vector was created as follows: 1) the 2.5 kb Gateway cassette attP2R-ccdB-cam-attP3 was PCR amplified from pDONR-P2R-P3 and cloned into pCR2.1-TOPO, generating plasmid pTOPO-attP2R-ccdB-attP3, 2) the P2R-ccdB-cam-P3 was excised from this plasmid and cloned as a HindIII/KpnI fragment into the HindIII/KpnI opened pPZP-RCS2 vector. The nucleotide sequence of the pOSCAR vector corresponds with the EMBL Nucleotide Sequence Database accession number HM623914.1. |
| EMBL Accession number: | HM623914.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/01/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII/KpnI, NotI and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. McCluskey(1). (1) FGSC, University of Missouri, Kansas City, USA |
| Plasmid reference: | Paz et al., Fungal Genet. Biol. 48 (2011), 677-684 [PMID: 21362493] [DOI: 10.1016/j.fgb.2011.02.003] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pOSCAR (LMBP 8055) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Paz et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.