Last data update: 24 January 2024 16:39 CET
Plasmid name: pT7POL21 (LMBP 3260)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage T7 RNA polymerase gene (T7g1) Phage λ cI temperature-sensitive repressor gene (cIts, cI857) phage λ nutL/N protein antitermination system |
| Promoter: | Phage λ major leftward promoter (λ PL) Phage λ major rightward promoter (λ PR) Phage λ promoter for repressor maintenance (λ PRM) |
| Ribosome binding site: |
- |
| Terminator: | Phage λ early leftward terminator (λ L) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Plasmid pSC101 origin derived from R6-5 |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | pLNR2; pAR1151; pLG339 |
| Further information: | The plasmid is a low-copy auxiliary vector for T7 promoter-based expression. The expression of the T7 RNA polymerase gene is controlled by the thermo-inducible λ PL promoter and is attenuated by one transcription terminator (the λ early leftward terminator) placed between the PL promoter and the coding sequence of the T7g1 gene. Upon induction, this attenuation can be overcome because of the presence of the phage λ nutL/N protein antitermination system. This provides a tighter control on gene expression. pT7POL21 carries the pSC101 replication origin. As a result, it is compatible with either ColE1-/pMB1- or p15A-derived plasmids, and as such, with the majority of expression vectors currently in use. This is an intermediate construction which is now replaced by pT7POL23. To use the plasmid, proceed as follows: first transform this auxiliary plasmid to the expression strain, make the transformants competent again and then transform the expression plasmid. Other name of the plasmid is pSCM21. |
| EMBL Accession number: | - |
| Latest sequence update: | 09/02/1996 |
| Authenticity test: | By lack of sequence data, it was not possible to perform the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr N. Mertens(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Mertens et al., Biotechnology 13 (1995), 175-179 [PMID: 9634760] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pT7POL21 (LMBP 3260) is available at BCCM/GeneCorner. This plasmid was deposited by Dr N. Mertens and Prof. Dr E. Remaut and was published in Mertens et al., 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.