Last data update: 24 January 2024 16:39 CET
Plasmid name: pUBQ10:lambdaN22-CFP-NLS-NPTII (LMBP 9019)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9019.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (ECFP) Phage λ N22 peptide ligand (lambdaN22, λN22); N-terminal SV40 large T-antigen nuclear localization signal (NLS); C-terminal |
Promoter: | Arabidopsis thaliana ubiquitin 10 promoter (UBQ10) Cauliflower mosaic virus (CaMV) 35S promoter |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin Pseudomonas plasmid pVS1 origin |
Host range: | Escherichia coli Plant cells Gram-negative bacterial strains |
Further information: | The λN22 protein recognises a 15 nt RNA stem-loop (box-B) with high specificity. Fused to the fluorescent ECFP tag, this system can be used to visualise the localisation of RNAs in plant cells. The plasmid contains the nptII gene which allows selection on neomycin in plant cells. Target RNAs can be introduced by Gateway recombination with p35S:16xBoxB-Gateway-BASTA (LMBP 9017). The λN22/box-B system is well suited for low-background and high-throughput studies. Other names of the plasmid are pSCJ387 and pUbiQ:IN22-CFP-NLS NPTII. |
EMBL Accession number: | - |
Latest sequence update: | 09/01/2015 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HincII, NotI and PstI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr U. Hammes(1). (1) Department of Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany |
Plasmid reference: | Schönberger et al., Plant J. 71 (2012), 173-181 [PMID: 22268772] |
Related plasmid reference: | Daigle et al., Nat. Methods 4 (2007), 633-636 [PMID: 17603490] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUBQ10:lambdaN22-CFP-NLS-NPTII (LMBP 9019) is available at BCCM/GeneCorner. This plasmid was deposited by Dr U. Hammes and was published in Schönberger et al., 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.