GREAT AT SMALL THINGS

GeneCorner plasmid details

Last data update: 25 May 2019 04:12 CEST  


Name: pUBQ10:lambdaN22-GFP-NLS-BASTA-RI   Add to cart
Accession number: LMBP 9020
Sequence file: Depositor's sequence: p9020.gb     View with Genome Compiler
Status: GeneCorner non-core plasmid
Cloned DNA: Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP)
Phage λ N22 peptide ligand (lambdaN22, λN22); N-terminal
SV40 large T-antigen nuclear localization signal (NLS); C-terminal
Promoter: Arabidopsis thaliana ubiquitin 10 promoter (UBQ10)
Cauliflower mosaic virus (CaMV) 35S promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Kanamycin (kan)
Phosphinotricin (basta)
Replicon: Escherichia coli plasmid pMB1 origin
Pseudomonas plasmid pVS1 origin
Host range: Escherichia coli
Plant cells
Gram-negative bacterial strains
Further information: The λN22 protein recognises a 15 nt RNA stem-loop (box-B) with high specificity. Fused to the fluorescent EGFP tag, this system can be used to visualise the localisation of RNAs in plant cells.
Target RNAs can be introduced by Gateway recombination with p35S:16xBoxB-Gateway-BASTA (LMBP 9017).
The λN22/box-B system is well suited for low-background and high-throughput studies.
Other names of the plasmid are pSCJ206 and LB-pUBI:lambdaN-GFP-NLS-BASTA RI.
EMBL Accession number: -
Latest sequence update: 09/01/2015
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, HincII, NotI and PstI.

As compared to the nucleotide sequence file, the HincII site at position 4139 appears to be absent, while an additional PstI site appears to be present.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr U. Hammes(1).
(1) Department of Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany
Plasmid reference: Schönberger et al., Plant J. 71 (2012), 173-181 [PMID: 22268772]
Related plasmid reference: Daigle et al., Nat. Methods 4 (2007), 633-636 [PMID: 17603490]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

 

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