Last data update: 24 January 2024 16:39 CET
Plasmid name: pUR250IVHA8s (LMBP 2178)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2178.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) haemagglutinin cDNA (HA, IVHA); N-terminal anchor free |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUR250; pUR250IVHA8 |
| Further information: | The plasmid was constructed as follows: a BamHI fragment from pUR250IVHA8, coding for secreted heamagglutinin (the last 36 amino acids are missing) was inserted into the unique BamHI site of pUR250. The orientation of the inserted fragment is such, that the HA8s gene can be cloned into expression vectors as BamHI or as SalI fragments. This haemagglutinin coding sequence is also called anchor-free haemagglutinin (HA(-)); it lacks the sequences coding for the intramembranous part and the cytoplasmic tail of the protein. This plasmid is a basic construction for all HA8s containing vectors. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Other names of the plasmid are pUR250H8A- and pUR250HA8s. |
| EMBL Accession number: | - |
| Latest sequence update: | 14/12/1989 |
| Sequence detail: | Sequence of the 3' end of the IVHA coding sequence:
IVHA ha2 chain -->|
180 186 |
5'...AAG.TCA.GGA.TAC.AAA.GAC.TGG.ATC.CGTCGACTCTAGAAAGCTT
BamHI SalI HindIII
XbaI |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by G. Maertens(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Huylebroeck et al., Gene 66 (1988), 163-181 [PMID: 2844629] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUR250IVHA8s (LMBP 2178) is available at BCCM/GeneCorner. This plasmid was deposited by G. Maertens and Prof. Dr W. Min Jou and was published in Huylebroeck et al., 1988. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.